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EC number: 200-712-3 | CAS number: 69-72-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to soil macroorganisms except arthropods
Administrative data
- Endpoint:
- toxicity to soil macroorganisms except arthropods: long-term
- Type of information:
- experimental study
- Adequacy of study:
- other information
- Reliability:
- 3 (not reliable)
- Rationale for reliability incl. deficiencies:
- other: Although conducted with pure test substance, the method used is far from the guidelines (mortality after 6 weeks exposure, more than one worm per vial, substrate not characterised...)
Data source
Reference
- Reference Type:
- publication
- Title:
- Effect of aromatic compounds, humic acids and lignins on growth of the earthworm Eisenia foetida.
- Author:
- Hartenstein R.
- Year:
- 1 982
- Bibliographic source:
- Soil Biol. Biochem. 14 : 595-599
Materials and methods
Test guideline
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- E. foetida hatchlings were exposed to a sludge substrate containing different concentrations in salicylic acid.
The impact on growth and on mortality of the test substance was recorded every two weeks during 6 weeks and at the end of the test (6weeks) respectively . - GLP compliance:
- not specified
Test material
- Reference substance name:
- Salicylic acid
- EC Number:
- 200-712-3
- EC Name:
- Salicylic acid
- Cas Number:
- 69-72-7
- Molecular formula:
- C7H6O3
- IUPAC Name:
- 2-hydroxybenzoic acid
- Details on test material:
- Test compound was purchased from Aldrich Chemical Co, Milwaukee, WI (USA).
Constituent 1
Sampling and analysis
- Analytical monitoring:
- not specified
Test substrate
- Vehicle:
- not specified
- Details on preparation and application of test substrate:
- The test substances were processed for assay by mixing 0.01, 0.1, 0.5 or 1.0 g or mL into 100 g sludge
Assuming exactly 13% solids in the sludge, the substances were thus tested at concentrations of about 0, 0.1, 1.0, 4 and 8 % (w/w) dry weight.
About 30g spiked sludge (average 13% solids) was placed over a 4 mm depth of silt loam in a 20*100 mm petri dish.
Test organisms
- Test organisms (species):
- Eisenia fetida
- Details on test organisms:
- Stock culture of Eisenia foetida were maintained on horse manure or activated sludge.
Two hatchlings of 10 mg live weight were used for the experiment
Study design
- Study type:
- laboratory study
- Substrate type:
- other: sludge
- Total exposure duration:
- 6 wk
- Remarks:
- measurements after 2, 4 and 6 wks
- Post exposure observation period:
- No post exposure period
Test conditions
- Test temperature:
- 24+-1°C.
- pH:
- no data
- Moisture:
- No exact data. Sludge contained 11 to 15% of solids, placed on silt loam layer.
- Details on test conditions:
- TEST SYSTEM
- Test container (material, size): petri dish of size 20 x 100 mm
- Amount of soil or substrate: About 30 g sludge (ca 13% solids) with test substance were placed over a ca 4 mm depth of silt loam in a 20*100 mm Petri dish.
- No. of organisms per container (treatment): Two hatchlings, each under 10 mg live weight, were added.
- No. of replicates per treatment group: 5 replicates
- No. of replicates per control: 5
SOURCE AND PROPERTIES OF SUBSTRATE
- Composition (if artificial substrate): sludge which contained 11-15% solids.
- Maximum water holding capacity (in % dry weigth): no data
- Stability and homogeneity of test material in the medium: solids are not homogeneously distributed in sludge, and solid concentration varies from sludge sample to sample and withn a single sample with time due to microbial activity
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Growth was checked every 2 weeks and worms were transferred to freshly prepared substance at 4 weeks.
Growth achieved at 2 or 4 and 6 weeks was determined by rinsing the worms in distilled water, blotting and weighing.
Mortality was determined by enumeration and said to be significant if 5 or more earthworms died.
TEST CONCENTRATIONS
- 5 concentrations tested - Nominal and measured concentrations:
- Assuming exactly 13% solids in the sludge, the substances were thus tested at concentrations of about 0, 0.1, 1.0, 4 and 8 % (w/w) dry weight.
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 6 wk
- Dose descriptor:
- LC100
- Effect conc.:
- 1 other: % dw of salicylic acid / sludge
- Nominal / measured:
- not specified
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Duration:
- 6 wk
- Dose descriptor:
- LOEC
- Effect conc.:
- < 1 other: % dw of salicylic acid /sludge
- Nominal / measured:
- not specified
- Conc. based on:
- test mat.
- Basis for effect:
- growth
- Details on results:
- Salicylic acid inhibited the growth at concentrations < 1% and gave 100% mortality at 1%.
Hatchlings in control dishes would gain more than 500 mg in 6 weeks. An average weight of 582 mg +/- 85 SD, with a cv of 14.6% was attained by controls in 6 wks in 45 similar assays during 16 months. This reflects both variation in earthworm growth and sludge quality. Mortality in controls was less than 2%. - Results with reference substance (positive control):
- no data
- Reported statistics and error estimates:
- Weight at 6 weeks was evaluated by analysis of variance.
Significant differences were determined by the Neuman-Keul test
Mortality was said to be significant if 5 or more earthworms died.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- not applicable
- Remarks:
- no guideline followed
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