Registration Dossier

Administrative data

Endpoint:
basic toxicokinetics in vivo
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference
Reference Type:
publication
Title:
Unnamed
Year:
1992

Materials and methods

Objective of study:
other: mechanistic
Test guideline
Qualifier:
no guideline followed
Principles of method if other than guideline:
The effect of Guanidine hydrochloride on energy metabolism was studied in rat liver, muscle and kidney.
GLP compliance:
not specified

Test material

Reference
Name:
Unnamed
Type:
Constituent
Radiolabelling:
no

Test animals

Species:
rat
Strain:
Wistar
Sex:
male

Administration / exposure

Route of administration:
intraperitoneal
Vehicle:
physiological saline
Duration and frequency of treatment / exposure:
daily for 7 d
Doses / concentrations
Remarks:
Doses / Concentrations:
0 (control), 2.2 mmol/kg bw/d
No. of animals per sex per dose:
8
Control animals:
yes, concurrent vehicle
Details on dosing and sampling:
After sacrifice liver, muscle and kidney were weighed homogenised and analysed for glycogen, lactic acid, pyruvate, lactate dehydrogenase, succinate dehydrogenase, glycogen phosphorylase

Results and discussion

Any other information on results incl. tables

- the glycogen content in liver, muscle and kidney decreased in treated rats (maximum effect in liver)

- elevated levels of lactic acid, decreased pyruvate levels in liver, muscle and kidney

- succinate dehydrogenase and glycogen phosphorylase activity was low in liver, muscle and kidney

- lactate dehydrogenase activity was decreased in liver and muscle and increased in kidney

- treatment with the test substance altered the carbohydrate metabolism by stimulating the glycolytic pathway.

Applicant's summary and conclusion

Conclusions:
Focus of the study was the investigation of carbohydrate metabolism.
Intraperitoneal injection of 2.2 mmol/kg bw/d Guanidinium hydrochloride for 7 d altered the carbohydrate metabolism in rat liver, muscle and kidney by stimulating the glycolytic pathway.