Ethyl chloroformate

Eye irritation

Administrative data

Endpoint:

eye irritation: in vivo

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Comparable to guideline study with acceptable restrictions. The study is sufficient for the evaluation of ethylchloroformate

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1970

Report date:

1970

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 405 (Acute Eye Irritation / Corrosion)

Deviations:

yes

Remarks:

: low volume of test article; does, however, not invalidate the result.

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

Ethyl chloroformate

EC Number:

208-778-5

EC Name:

Ethyl chloroformate

Cas Number:

541-41-3

Molecular formula:

C3H5ClO2

IUPAC Name:

ethyl chlorocarbonate

Details on test material:

- Name of test material (as cited in study report): Aethylchlorkohlensäureester (ethy carbonochloric acid ester)
- Substance type:
- Physical state: liquid
- Analytical purity: ca. 95 %
- internalTest substance No. XIX/351.

Test animals / tissue source

Species:

rabbit

Strain:

Vienna White

Details on test animals or tissues and environmental conditions:

TEST ANIMALS
- Source: Gaukler, Offenbach, Germany
- Weight at study initiation: 2.0 kg (f), 2.0 kg (m), 3.0 kg (m)
- Housing: single
- Diet (e.g. ad libitum): ad libitum
- Water (e.g. ad libitum): ad libitum

ENVIRONMENTAL CONDITIONS
no data

Test system

Vehicle:

unchanged (no vehicle)

Controls:

not required

Amount / concentration applied:

0.05 ml

Duration of treatment / exposure:

application ito the conjunctival sack, no washing afterwards

Observation period (in vivo):

8 days

Number of animals or in vitro replicates:

4

Details on study design:

REMOVAL OF TEST SUBSTANCE
- Washing (if done): no washing done

SCORING SYSTEM:
Comparable to the draize scoring system

TOOL USED TO ASSESS SCORE: luorescein

Results and discussion

In vivo

Results

Results 1

Irritation parameter:

cornea opacity score

Basis:

mean

Time point:

other: 24 - 72 h

Score:

2

Max. score:

3

Reversibility:

not reversible

Results 2

Irritation parameter:

iris score

Basis:

mean

Time point:

other: 24 - 72 h

Score:

1.4

Max. score:

2

Reversibility:

not reversible

Results 3

Irritation parameter:

conjunctivae score

Basis:

mean

Time point:

other: 24 - 72 h

Score:

1.7

Max. score:

2

Reversibility:

not reversible

Results 4

Irritation parameter:

chemosis score

Basis:

mean

Time point:

other: 24 - 72 h

Score:

2.8

Max. score:

4

Reversibility:

not reversible

Irritant / corrosive response data:

Severe eye irritation and corneal opacity was seen at 1 h after application and persisted until the end of the observation period on day 8 after treatment.

Any other information on results incl. tables

Descriptive scores of the raw data have been converted to Draize numerical scores.

Animal	Reading	Opacity	Iritis	Redness	Chemosis	Comment
1	1 h	3	0	2	2
2	1 h	1	0	2	3
3	1 h	3	0	1	2
4	1h	3	0	2	4
1	24 h	3	0	2	2	hemorrhage
2	24 h	2	0	2	3
3	24 h	3	0	1	3
4	24 h	3	2	2	3	hemorrhage
1	48 h	2	2	2	2	hemorrhage
2	48 h	-	-	-	-	no data
3	48 h	-	-	-	-	no data
4	48 h	2	2	2	3	hemorrhage
1	72 h	2	2	2	3	hemorrhage
2	72 h	2	2	2	3
3	72 h	3	2	1	3
4	72 h	2	2	2	3	hemorrhage
1	8 d	3	2	2	1	staphyloma/suppuration
2	8 d	3	2	2	1	staphyloma/suppuration
3	8 d	3	2	2	1	staphyloma/suppuration
4	8 d	3	2	2	1	staphyloma/suppuration
mean	24 - 72 h	2,4	1.4	1.7	2.80

 

Applicant's summary and conclusion

Interpretation of results:

corrosive

Remarks:

Migrated information

Conclusions:

The results indicate the risk of serious damage to eyes.