N-butylbenzenesulphonamide

Bioaccumulation: aquatic / sediment

Link to relevant study record(s)

Reference

Endpoint:

bioaccumulation in aquatic species: fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

November 1998

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study

Justification for type of information:

OECD 305 is the standard test method in fish of Bioaccumulation.

Qualifier:

according to guideline

Guideline:

OECD Guideline 305 E (Bioaccumulation: Flow-through Fish Test)

GLP compliance:

yes (incl. QA statement)

Specific details on test material used for the study:

Purity of N-butylbenzenesiulfonamide 100.1 %
Verification of the Test Substances
The structure was confirmed by the infrared absorption spectrum , mass spectrum and nuclear magnetic resonance spectrum.

Radiolabelling:

no

Vehicle:

no

Test organisms (species):

Cyprinus carpio

Details on test organisms:

Specimen Fish
Fish Type :Carp Cyrinus carpio
Supply source :Sugijima Nurseries
(Address 866-0024 123-2, Gunchiku Ichibancho, Yatsushiro District, Kumamoto Prefecture)
Reception Date of the Specimen Fish March 20, 1998


Nursery condition
When the fish were obtained, the visual observation was carried out to remove abnormal substances, and
after receiving the medicinal bath in the receiving tank, they were raised for 61 days under the running water condition.

Acclimatization condition
After the farming, and after carrying out the chemical bath for parasites extermination, they were brought to the acclimatization tank, and after carrying it out the medicinal bath again, the acclimatization was performed. Meanwhile, those with abnormalities were removed and they were raised for 60 days at a water temperature of 25±2°C. Furthermore, they were moved to a test water tank, and after the medicinal bathing, they were kept for 29 days under the flowing water condition at the same temperature.

Body weight and body length before the exposure
Body weight Average 23.3g
Body length Average 9.5cm
Lipid content rate Average 3. 8%

Lot Measurement Value of TFC-980 320-II
Measurement Date July 15, 1998
Feed
Type :Pellet formulated feed for carp
Manufacturer : Nippon Formula Feed Mfg. Co., Ltd.
Feeding Method
Body weight of the specimen fish of approximately 2% of the equivalent weight was fed twice a day. However, the feeding was stopped on the day before the sampling of the specimen fish.

Route of exposure:

aqueous

Test type:

flow-through

Total exposure / uptake duration:

ca. ca. 42 d

Hardness:

Test Water
Type
Groundwater pumped up in the Kurume laboratory premise
Water quality check
Water sampling was performed on August 3, 1998, at our laboratory and the results of the measurement or analysis were shown in Reference 1 (measurement frequency is 1 time / 6 months). Measured or analyzed values of each items have been verified to be under the concentration described in the “Water quality standards based on the Waterworks Act” (Ministry of Health and Welfare, Ordinance No. 69 revised on December 21, 1992), “Fishery Water Standards” (Japan Aquatic Resource Protection Society, March 1988) or “OECD Guidelines for Testing of Chemicals 210, Fish, Early-life Stage Toxicity Test” (July 17, 1992).

Test temperature:

25°C+/- 2°C

Dissolved oxygen:

first concentration zone 7.4-8.0 mg/l
second concentration zone 7.6- 8.1 mg/L
control zone 7.9-8.1 mg/l

Details on test conditions:

Observation, Measurement, etc.

Observation of specimen fish :
Health condition of the specimen fish was visually observed twice a day.
Test water volume :
Measurement was recorded once a day by using the measuring cylinder.
Test water temperature :
Measurement was recorded once a day by using the alcohol thermometer.
Dissolved oxygen concentration:
Measurement was recorded twice a week by using the dissolved oxygen meter.
Others
During the test period, the carp drainage, stain on the aquarium wall etc. were removed about once a day.

Nominal and measured concentrations:

Concentration in µg/L Week 2 in µg/L Week 3 in µg/L Week 4 in µg/L Week 6 in µg/L
Nominal : 20 19.6 19.5 19.20 18.6
Nominal : 2 1.97 1.97 1.96 1.94

Reference substance (positive control):

no

Details on estimation of bioconcentration:

the bioconcentration factor is calculated as the ratio of concentrtion in fish and in the water

Lipid content:

3.8 %

Time point:

other: not specified

Key result

Conc. / dose:

2 µg/L

Type:

BCF

Value:

3.6 L/kg

Basis:

not specified

Time of plateau:

6 wk

Key result

Conc. / dose:

20 µg/L

Type:

BCF

Value:

3.3 L/kg

Basis:

not specified

Time of plateau:

6 wk

Key result

Elimination:

yes

Parameter:

other: 98.8%

Remarks:

on average 98.8% ( 2 points) of the test substance was measured in the water

Remarks on result:

other: 90.%

Remarks:

on average ( 2 points) 90 % of the test substance was lossed measured in the specimen

Details on results:

Concentration ratio ( BCF values)

Concentratione Week 2 Week 3 Week 4 Week 6
20 µg/L 2.7 3.3 3.2 1.4
2.8 2.2 2.2 1.9

2 µg/L Less than 3.6 Less than 3.6 Less than 3.6 Less than 3.6
Less than 3.6 Less than 3.6 Less tha 3.6 Less than 3.6


The water- to-fish ratio ( test substance into the carp) was between 1.4 and 3.3 times in the 20 µ/L test, and less than 3.6 times in the 2 µg/L .
No abnormalities were found in the specimen Fish.

Validity criteria fulfilled:

yes

Conclusions:

The bioaccumulation potential of the substance N-butylbenzenesulfonamide (BBSA) was determined in carp cyrinus carpio according to the OECD 305. Taking into consideration the 48 hour LC50 (53.2 mg/L ) and the analytical sensitivity of the test substance the test substance concentration was set on : 20 µg/L and 2 µg/L. In a 100 L glass water tank ( flow through) the water-to-fish up load was measured at 25 °C at an exposure time of 6 weeks. Preliminary investigation conducted before the exposure, confirmed that the substance was stable under the test conditions. The concentration of (BBSA) was measured in the water( CF) and in the fish (CW) over a period of six weeks. The concentration ratios were calculated ( BCF= CF/CW) for the different time points. The degree of concentration towards the test specimen (Carp) was between 1.4 and 3.3 times for 20 µg/L, and less than 3.6 times for the 2µg/L test. No abnormalities were observed in the carp over a time period of 6 weeks of exposure.
In addition the recovery (depuration) during which the net loss of the substance (BBSA) from the fish into the water (with no additional test substance) was studied. The recovery was measured on two points. The recovery rate was 98.8 % ( average) on the water analysis, and 90.0 % on the fish.

Executive summary:

The BCF factor of N-buylbenzenesulfonamide was measured according to OECD 305 quidelines . Two dose levels ( 2 and 20 µg/L) were studied. A group of fish (Cyprinus carpio fish), with a lipid content of 3.8 % were exposed to the test substance. A steady-state has been reached after 6 weeks.The BCF factor was calculated as 1.4 -3.3 L/kg (20 µg/L) and less than 3.6 L/kg (2 µg/L). In addition the carp were transferred to a medium free of the test substance for the depuration phase. This depuration was studied over two endpoints. It was concluded that 98.8 % of the test substance was found in water. And a loss of 90.0 % in the fish.

Description of key information

The bio-accumulation potential of N-buylbenzenesulfonamide was measured according to OECD 305 E quidelines. Two dose levels (2 and 20 µg/L) were studied. A steady-state has been reached after 6 weeks. The BCF factor was calculated as 1.4 -3.3 L/kg (20 µg/L) and less than 3.6 L/kg (2 µg/L). In addition the carp were transferred to a medium free of the test substance for the depuration phase. This depuration was studied over two endpoints. It was concluded that 98.8 % of the test substance was found in water and a loss of 90.0 % in the fish was observed.

Key value for chemical safety assessment

BCF (aquatic species):

3.6 L/kg ww