Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 402-920-1 | CAS number: 103300-89-6 N-6-TRIFLUORACETYL-L-LYSYL-L-PROLIN; T.F.A. LYS PRO; TFA-LYS-PRO
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1996-01-10 to 1996-04-19
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Version / remarks:
- 1984
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: During the test duplicate samples were taken from 0 and 100 mg/L for analysis.
- Sampling method: Sampling: Frequency at t=0h (extra sample at 100 mg/L) and t=48h. Volume 10 mL from the approximate centre of the test vessel.
- Sample storage conditions before analysis: Samples not analyzed on the day of sampling were stored in a deep freeze. Specification of the samples analysed and the method of analysis are described in the appended Analytical Report. - Vehicle:
- yes
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The test solutions were prepared using a stock solution of 100 mg/L by adding quantitatively 25.1 mg of test substance to 250 mL ISO-medium. The final test solution was all clear without precipitation. - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Water flea
- Strain/clone: Daphnia magna (Crustacea, Cladocera) (Straus, 1820)
- Age at study initiation (mean and range, SD): < 24h
- Method of breeding: Start of each batch with newborn animals, i.e. less than 3 days old, by placing about 250 of them into 10 litres of medium in an all-glass culture vessel. Maximum age of the cultures 4 weeks. Renewal of the cultures. After 7 days of cultivation half of the medium twice a week.
- Feeding during test None
ACCLIMATION
- Acclimation period: n/a inhouse culture
- Acclimation conditions (same as test or not): same as test
- Type and amount of food: a suspension of fresh water algae.
- Feeding frequency: daily
- Health during acclimation (any mortality observed): not reported
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Hardness:
- 250 mg/L CaCO3
- Test temperature:
- between 20.0 and 20.5 °C
- pH:
- between 7.8 and 8.4
- Dissolved oxygen:
- between 8.8 and 9.3 mg/L
- Nominal and measured concentrations:
- Nominal concentrations: 0, 0.1, 1.0, 10, 100, 1000 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: glass vessels
- Material, size, headspace, fill volume: glass, volume: 100 mL, fill volume: 80 mL
- Aeration: No
- No. of organisms per vessel: 20 in the controls and at 100 mg/L; 10 at 0.1, 1, and 10 mg/L
- No. of vessels per concentration (replicates): duplicate
- No. of vessels per control (replicates): duplicate
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: M4, as prescribed by Dr. Elendt-Schneider (Elendt, B.-P., 1990: Selenium deficiency in Crustacea. An ultrastructural approach to antennal damage in Daphnia magna Straus. Protoplasma 154, 25-33).
- Culture medium different from test medium: No
OTHER TEST CONDITIONS
- Adjustment of pH: No
- Photoperiod: 16/8
- Light intensity: not reported
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
VEHICLE CONTROL PERFORMED: yes/no
RANGE-FINDING STUDY
- Test concentrations:
- Results used to determine the conditions for the definitive study: - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Validity criteria fulfilled:
- yes
- Conclusions:
- In a test conducted according to OECD guideline 202 (1984), daphnids were treated with the test substance up to a concentration of 1000 mg/L for 48h. The 48h EC50 is considered to be > 1000 mg/L (nominal concentration).
Reference
Description of key information
In a test conducted according to OECD guideline 202 (1984), daphnids were treated with ε-trifluoracetyl-L-lysyl-L-proline (TFA-Lys-Pro) up to a concentration of 1000 mg/L for 48h. The 48h EC50 is considered to be > 1000 mg/L (nominal concentration).
Key value for chemical safety assessment
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.