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EC number: 402-920-1 | CAS number: 103300-89-6 N-6-TRIFLUORACETYL-L-LYSYL-L-PROLIN; T.F.A. LYS PRO; TFA-LYS-PRO
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1992-12-17 to 1993-03-31
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 E (Ready biodegradability: Modified OECD Screening Test)
- Version / remarks:
- 1990
- GLP compliance:
- yes
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic (adaptation not specified)
- Details on inoculum:
- - Preparation of inoculum for exposure: The original sludge was homogenized and contained about 2.8 g of solid substance per
litre. 193 mL of the mixed sludge was used to inoculate 18 litres of medium (yielding about 30 mg of solid substance L-1).
- Concentration of sludge: 2.8 g/L - Duration of test (contact time):
- 28 d
- Initial conc.:
- 10 mg/L
- Based on:
- test mat.
- Initial conc.:
- 20 mg/L
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- DOC removal
- Details on study design:
- TEST CONDITIONS
- Composition of medium:
Add to one litre water 10 ml of solution A and 1 mL of solutions B to F.
a) Solution A contains per litre:
KH2PO4 8.5 g
K2HPO4 21.8 g
Na2HPO4 x 2 H2O 33 .4 g
NH4Cl 1.5 g
b) Solution B contains 22.5 g of MgSO4 x 7 H2O per litre.
c) Solution C contains 27.5 g of CaCl2 x 2 H2O per litre.
d) Solution D contains 0.15 g of FeCl3 per litre.
e) Trace element solution.
This solution contains per litre water:
MnSO4 x 4 H2O 39.9 mg
H3BO3 57.2 mg
ZnSO4 x 7 H2O 42.8 mg
(NH4)6Mo7O24 34.7 mg
EDTA 100.0 mg
f) 15 mg yeast extract dissolved in 100 mL water.
Sterilize solutions A, B, C and E. Solutions D and F are prepared freshly before use.
- Test temperature: 20 ± 2 °C
- pH: 7.6 ± 0.4
- pH adjusted: no
- Aeration of dilution water: Yes, 24h before the start of the test
- Continuous darkness: yes
TEST SYSTEM
- Number of culture flasks/concentration: duplicate
- Measuring equipment: O C International 700 TOC analyzer
- Test performed in open system: Two litre erlenmeyer flasks (Scott Duran), closed with a foam cap
SAMPLING
- Sampling frequency: 0, 7, 14, 21, 28 days
- Sterility check if applicable: sterility control flask contained uninoculated medium, 10 mg test substance and 5 mL 1% mercury(II)chloride solution.
- Sample storage before analysis: The samples were placed in scintillation vials (20 mL) and frozen (-20°C) pending analyses.
CONTROL AND BLANK SYSTEM
- Inoculum blank: Control flasks without test substance to allow correction for the background DOC in the medium were included in duplicate.
- Abiotic sterile control: Yes, a sterile control was included in the test; this flask contained uninoculated medium, 10 mg test substance and
5 mL 1% mercury(II)chloride solution.
- Toxicity control: In order to detect possible toxic effects of the test substance, sodium acetate (100 mg/L) was also added to further duplicate flasks containing 10 and 20 mg/L of ε-trifluoracetyl-L-lysyl-L-proline (TFA-Lys-Pro).
- Reference substance:
- acetic acid, sodium salt
- Parameter:
- % degradation (DOC removal)
- Value:
- 52
- Sampling time:
- 7 d
- Parameter:
- % degradation (DOC removal)
- Value:
- 93
- Sampling time:
- 14 d
- Key result
- Parameter:
- % degradation (DOC removal)
- Value:
- 100
- Sampling time:
- 28 d
- Details on results:
- The results of this test show that, as expected, acetate was completely degraded (99%) within one week.There were no effects of the test substance of the acetate degradation. The test substance degraded up to 99% within 28 days in this test. The second criterium for ready biodegradability (the 10 day window) was also met
- Results with reference substance:
- 99 % degradation after 7 d (DOC removal)
100 % degradation after 14 d (DOC removal)
100 % degradation after 21 d (DOC removal) - Validity criteria fulfilled:
- yes
- Interpretation of results:
- readily biodegradable
- Conclusions:
- In a study conducted according to OECD guideline 301E (1990) the biodegradability was determined by DOC removal. The test substance degraded up to 99% within 28 days in this test. The second criterium for ready biodegradability (the 10 day window) was also met.
It was therefore concluded that ε-trifluoracetyl-L-lysyl-L-proline (TFA-Lys-Pro) can be regarded as readily biodegradable in this test.
Reference
Results ofthe biodegradation tests with ε-trifluoracetyl-L-lysyl-L-proline (TFA-Lys-Pro).
|
ε-trifluoracetyl-L-lysyl-L-proline (mg/L)
|
|||
|
10 |
20 |
||
|
mg DOC |
%DOC removal |
mg DOC |
%DOC removal |
0 |
3.4 |
- |
7.2 |
- |
7 |
1.6 |
52 |
1.0 |
86 |
14 |
0.3 |
93 |
1.0 |
86 |
21 |
0.4 |
90 |
0.6 |
92 |
28 |
-0.2 |
100 |
0.2 |
98 |
Description of key information
In a study conducted according to OECD guideline 301E (1990) the biodegradability was determined by DOC removal. ε-trifluoracetyl-L-lysyl-L-proline (TFA-Lys-Pro) degraded up to 99% within 28 days in this test. The second criterium for ready biodegradability (the 10 day window) was also met.
Therefore, ε-trifluoracetyl-L-lysyl-L-proline (TFA-Lys-Pro) can be regarded as readily biodegradable.
Key value for chemical safety assessment
- Biodegradation in water:
- readily biodegradable
- Type of water:
- freshwater
Additional information
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