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EC number: 430-320-1 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 27th - 31st July 1998
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 998
- Report date:
- 1998
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- Pentaerythritol mixed esters with 2-methyl butyric acid, n-pentanoic acid, n-heptanoic acid, 3,5,5-trimethylhexanoic acid, n-octanoic acid, n-decanoic acid
- Cas Number:
- 141686-49-9
- IUPAC Name:
- Pentaerythritol mixed esters with 2-methyl butyric acid, n-pentanoic acid, n-heptanoic acid, 3,5,5-trimethylhexanoic acid, n-octanoic acid, n-decanoic acid
- Reference substance name:
- Trimethylol propane mixed esters with 2-methyl butyric acid, n-pentanoic acid, n-heptanoic acid, 3,5,5-trimethylhexanoic acid, n-octanoic acid, n-decanoic acid
- Cas Number:
- 141686-50-2
- IUPAC Name:
- Trimethylol propane mixed esters with 2-methyl butyric acid, n-pentanoic acid, n-heptanoic acid, 3,5,5-trimethylhexanoic acid, n-octanoic acid, n-decanoic acid
- Test material form:
- liquid
- Details on test material:
- Identification: Hatcol ® 1772
Appearance/physical state: Clear colorless liquid
Purity: 100% (no solvent present)
Composition: 70% pentaerythritol esters described by CAS# 141686-49-9; 30% trimethylolpropane esters described by CAS# 141686-50-2
Constituent 1
Constituent 2
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- PREPARATION OF STANDARDS
Standard solutions of test material were prepared in hexane at a nominal concentration of 1000 mg/I.
PREPARATION OF TEST SAMPLES
A volume of sample was extracted with three portions (3 x 50 ml) of dichloromethane and the extracts filtered through anhydrous sodium sulphate. The combined extracts were evaporated to dryness and the residue re-dissolved in hexane.
STABILITY TESTING
A range of pre-study test samples was prepared in bottles and analysed initially and then after storage at ambient temperature in light and dark conditions for approximately 96 hours (equivalent to the study exposure period). The samples were prepared by "spiking" known volumes of medium with test material. Pre-study WAFs were prepared and analysed initially and then after storage at ambient temperature in light and dark conditions for approximately 96 hours (equivalent to the study exposure period).
Test organisms
- Test organisms (species):
- Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- The test was carried out using Pseudokirchneriella subcapitata strain CCAP 278/4. Liquid cultures of Pseudokirchneriella subcapitata were obtained from the Culture Collection of Algae and Protozoa (CCAP), Institute of Freshwater Ecology, Ferry House, Ambleside, Cumbria. Cultures were maintained in the laboratory by the periodic replenishment of culture medium. The culture was maintained in the laboratory at a temperature of 21 ± 1°C under continuous illumination (intensity approximately 7000 lux) and constant aeration.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 96 h
Test conditions
- Test temperature:
- 24 ± 1°C
- pH:
- The pH values of the control cultures were observed to increase from pH 7.5 at 0 hours to pH 9.1 - 10.0 at 96 hours. The increase in pH observed after 96 hour is considered not to affect the integrity of the study, given that the validation criterion for control cell multiplication was exceeded.
Results and discussion
Effect concentrationsopen allclose all
- Key result
- Duration:
- 96 h
- Dose descriptor:
- EL50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Key result
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Key result
- Duration:
- 96 h
- Dose descriptor:
- EL50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
Any other information on results incl. tables
Range-finding study
The results showed no effect on growth at the test concentrations of 10 and 100 mg/l loading rate WAF. Preliminary work conducted for the validation of mixing period indicated that there was no significant increase in the amount of Total Organic Carbon (TOC) in the WAF by extending the mixing period for longer than 24 hours.Therefore a 24-hour mixing period with an hour settlement period was considered appropriate for the test. Based on this information a single test concentration of six replicates, of 100 mg/l loading rate WAF was selected for the definitive study. This experimental design conforms to a "limit test" to confirm that at the maximum test concentration given in the test guidelines no effect on growth was observed.
Definitive study
Growth data: From the data it is clear that neither the growth rate (r) or the biomass (b) of Pseudokirchneriella subcapitata (CCAP 276/20) were affected by the presence of the test material over the 96 -hour exposure period. The ELR50 value with respect to biomass, EbLR50 (72 and 96 h), was determined by inspection of the area under the growth curve data after 72 and 96 hours. The EC50 value with respect to the growth rate, EbLR50 (0 - 96 h), was determined by inspection of the growth rates for the period 0 - 96 hours.
Accordingly the following results were determined from the data:
EbLR50 (96 h) - >100 mg/l loading rate WAF
ErC50 (0 - 96 h) - 100 mg/l loading rate WAF
where EbLR50 is the test concentration that reduced biomass by 50% and ErC50 is the test concentration that reduced specific growth by 50%.
Statistical analysis of the area under the growth curve data was carried out for the control and 100 mg/l loading rate WAF test group using the Students t-test. There were no statistically significant differences (P ≥ 0.05), between the control and 100 mg/l loading rate WAF test group and therefore the 'No Observed Effect Concentration' (NOEC) is given as equal to 100 mg/l loading rate WAF. It was considered unnecessary and unrealistic to test at concentrations in excess of 100 mg/l loading rate WAF. The following data show that the cell concentration of the control cultures increased by a factor of 65 at 72 hours and by a factor of 231 at 96 hours during the test in line with the OECD Guideline that states the enhancement must be at least by a factor of 16 after 72 hours, and the EPA Guidelines that states the enhancement must be at least by a factor of 100 after 96 hours:
Mean cell density of control at 0 hours: 1.22 x 104 cells per ml
Mean cell density of control at 72 hours: 7.96 x 105 cells per ml
Mean cell density of control at 96 hours: 2.82 x 106 cells per ml
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- Exposure of Pseudokirchneriella subcapitata to the test material gave EL50 values of greater than 100 mg/L loading rate WAF. Correspondingly, the No Observed Effect Concentration (NOEC) was equal to 100 mg/L. It was considered unnecessary and unrealistic to test at concentrations in excess of 100 mg/L loading rate WAF.
- Executive summary:
Introduction
A study was performed to assess the effect of the test material on the growth of the green alga Pseudokirchneriella subcapitata. The method followed that described in the OECD Guidelines for Testing of Chemicals (1984) No 201; US CFR Title 40, Part 797, Section 1050; US EPA Pesticide Assessment Guideline, Sub-division J,Section 122 -2 and US EPA Draft Ecological Effects Test Guidelines OPPTS 850.5400.
Procedure
Following a preliminary range-finding study, Pseudokirchneriella subcapitata was exposed to a Water Accommodated Fraction (WAF) at a single loading rate of 100 mg/l (six replicate flasks) for 96 hours, under constant illumination and shaking at a temperature of 24 ± 1°C. Samples of the algal populations were removed daily and cell concentrations determined for each control and treatment group using a Coulter® Multisizer II Particle Counter.
Results
Exposure of Pseudokirchneriella subcapitata to the test material gave EL50 values of greater than 100 mg/l loading rate WAF. Correspondingly, the No Observed Effect Concentration (NOEC) was equal to 100 mg/l. It was considered unnecessary and unrealistic to test at concentrations in excess of 100 mg/l loading rate WAF.
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