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The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Toxicological information

Acute Toxicity: oral

Currently viewing:

Administrative data

Endpoint:
acute toxicity: oral
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1980
Report date:
1980

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 401 (Acute Oral Toxicity)
Principles of method if other than guideline:
Six groups of 5 male and 10 female young adult Wister rats (average weight 175 g) each received per gavage a single dose of 100, 500, 1000, 1500, 2000 or 2500 µl/kg bw (= ca. 109.5, 547.5, 1095, 1642.5, 2190, 2737.5 mg/kg) of undiluted phenyl isocyanate. The animals were observed for mortality, body weight and clinical signs through day 14.
GLP compliance:
no
Test type:
standard acute method
Limit test:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
Phenyl isocyanate
EC Number:
203-137-6
EC Name:
Phenyl isocyanate
Cas Number:
103-71-9
Molecular formula:
C7H5NO
IUPAC Name:
isocyanatobenzene

Test animals

Species:
rat
Strain:
Wistar
Sex:
male/female

Administration / exposure

Route of administration:
oral: gavage
Vehicle:
unchanged (no vehicle)
Doses:
100, 500, 1000, 1500, 2000 or 2500 µl/kg (= ca. 109.5, 547.5, 1095, 1642.5, 2190, 2737.5 mg/kg)
density = 1,095 kg/L (20 °C)
No. of animals per sex per dose:
5 male and 5 female animals/sex/dose
Control animals:
no

Results and discussion

Effect levels
Key result
Sex:
male/female
Dose descriptor:
LD50
Effect level:
0.81 mL/kg bw
Based on:
test mat.
Remarks on result:
other: 0.81 ml/kg bw = 887 mg/kg bw
Mortality:
Males: 0 (0.1 ml/kg bw), 2 (0.5 ml/kg bw), 3 (1.0 ml/kg bw), 4 (1.5 ml/kg bw), 4 (2.0 ml/kg bw), 5 (2.5 ml/kg bw)
Females: 0 (0.1 ml/kg bw), 2 (0.5 ml/kg bw), 2 (1.0 ml/kg bw), 3 (1.5 ml/kg bw), 4 (2.0 ml/kg bw), 5 (2.5 ml/kg bw)
Clinical signs:
other: A single dose of 0.5 to 2.5 ml/kg bw caused in all animals weight reduction, anesthesia and decrease of the general condition. At a dose of 1.0 to 2.5 ml/kg bw cyanosis and disordered breathing was additionally observed. The symptoms occurred after 10 min
Gross pathology:
No data
Other findings:
No data.

Any other information on results incl. tables

Signs of intoxication: Cyanosis, weight reduction, disordered breathing, reduction of general condition. No significant differences in male and female rats were observed.

Applicant's summary and conclusion

Interpretation of results:
Category 4 based on GHS criteria
Conclusions:
For male and female rats a LD50 = 0.81 ml/kg bw = 887mg/kg bw was found. According to CLP classification criteria (Regulation (EC) No 1272/2008) a classification as Acute Tox. 4 is justified.
Executive summary:

Six groups of 5 male and 10 female young adult Wister rats (average weight 175 g) each received per gavage a single dose of 100, 500, 1000, 1500, 2000 or 2500 µl/kg bw of undiluted phenyl isocyanate. The animals were observed for mortality, body weight and clinical signs through day 14.

Signs of intoxication were cyanosis, weight reduction, disordered breathing and a reduction of the general condition.

For male and female rats a LD50 = 0.81 ml/kg bw = 887mg/kg bw was found.