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EC number: 429-290-0 | CAS number: 3380-30-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1999-12-20 to 1999-01-04
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP, OECD guideline study.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 999
- Report date:
- 1999
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Version / remarks:
- adopted 1984
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Version / remarks:
- 1992
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- -
- EC Number:
- 429-290-0
- EC Name:
- -
- Cas Number:
- 3380-30-1
- Molecular formula:
- C12 H8 Cl2 O2
- IUPAC Name:
- 5-chloro-2-(4-chlorophenoxy)phenol
- Details on test material:
- - Name of test material (as cited in study report): FAT 80'220/A
- Physical state: solid powder
- Batch no.: GRU 98
- Expiration date: October 31, 2008
- Purity: >99%
- Solubility in water: 19.5 mg/L (determined in RCC Project 712012)
- Stability in water: >1 year (estimated half-life at 25 °C; determined in RCC Project 712260)
- pH in aqueous solution: 5-7 at a concentration of 0.01 g/L
- Storage conditions: at room temperature at about 20 °C, away from direct sunlight
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- For analytical measurements of the test item concentrations, one sample from the freshly prepared stock solution and duplicate samples from the freshly prepared test media of all test concentrations and the control were taken just before the start of the test (without daphnids) for analysis.
For determination of the stability of the test item under the test conditions, respectively the maintenance of the test item concentrations during the test period, sufficient volumes of the freshly prepared test media of all test concentrations and the control were incubated during the test period under the same conditions as in the actual test (but without daphnids). Duplicate samples were taken at the end of the test period. The collecting of samples after 48 hours from the actual test itself was not possible, since the test media volumes in the test were too small for the analytical requirements. All samples were deep-frozen (at about -20 °C) immediately after sampling.
The concentrations of the test item DCPP were analyzed in the stock solution sample and in the duplicate test media samples from the test concentrations of nominal 0.22 to 0.46 mg/L and both sampling times (0 and 48 hours). The lowest test item concentration of nominal 0.1 mg/L was not analysed, since it was below the 48-hour NOEC. The highest test item concentrations of nominal 1.0 and 2.2 mg/L were not analysed, since after 48-hours the same toxic effect was determined at the next lower test item concentration of nominal 0.46 mg/L. The test item concentration of nominal 1.0 and 2.2 mg/L therefore were considered as being of no biological relevance for the concentration-effect relationship. From the control samples only one of the duplicate samples was analysed from each of both sampling times.
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
A stock solution of nominal 10 mg/L was prepared by suspending 10 mg of the test item in about 100 mL test water by ultrasonic treatment for 5 minutes first. The suspension was made up to a volume of 1000 mL with test water and dissolved by ultrasonic treatment for 15 minutes and intense stirring for 3 hours. Adequate volumes of the intensively mixed stock solution were added to test water to prepare the following nominal test concentrations: 0.1, 0.22, 0.46, 1.0, and 2.2 mg/L.
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia magna Straus
- Original source: University of Sheffield/UK in 1992. Since this date the clone is bred in the laboratories of RCC.
- Age at study initiation (mean and range, SD): 6 to 24 hours
- Method of breeding: bred in the laboratories of RCC in reconstituted water of identical quality (regarding pH, main ions and total hardness) and under identical temperature and light conditions as in the tests
- Feeding during test: none
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
Test conditions
- Hardness:
- 2.5 mmol/l (= 250.0 mg/L) as CaCO3
- Test temperature:
- 20-21 °C during the test period
- pH:
- 7.8 to 7.9
- Dissolved oxygen:
- 8.5 mg/L
- Nominal and measured concentrations:
- - nominal concetration: 0.1, 0.22, 0.46, 1.0 and 2.2 mg/L
- measured concentration: 81 to 87% of the nominal values, therefore all reported values refer to the nominal concentrations - Details on test conditions:
- TEST SYSTEM
- Test vessel: 100-mL glass-beaker
- Type (delete if not applicable): closed
- Aeration: no aeration during the test
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Reconstituted test water
- Alkalinity: 0.8 mmol/L
- Ca/mg ratio: 4:1 (based on molarity)
OTHER TEST CONDITIONS
- Photoperiod: a 16-hour light to 8-hour darkness photoperiod
- Light intensity: light intensity at light period between 200 and 1200 Lux
TEST CONCENTRATIONS
- The test concentrations were based on the results of a 5 day range-finding test and the results of a pre-experiment to the solubility of the test item (without GLP). - Reference substance (positive control):
- no
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 0.32 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: 0.22-0.46
- Duration:
- 48 h
- Dose descriptor:
- EC0
- Effect conc.:
- 0.22 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.22 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC100
- Effect conc.:
- 0.46 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- MEASURED CONCENTRATIONS
The analytical mean test item concentrations in the analysed test media varied in the range from 81 to 87% of the nominal values. In the test media the test item was sufficiently stable during the test period of 48 hours. Therefore, all reported biological results were related to the nominal test item concentrations
IMMOBILITY
In the control and up to and including the test item concentration of nominal 0.46 mg/L no immobility or mortality of the test animals or other signs of intoxication were determined during the test period of 24 hours. After 48 hours of exposure the toxicity of the test item to Daphnia magna had increased.
CONTROL ANIMALS
No abnormalities occurred in the control groups.
Any other information on results incl. tables
Influence of DCPP on the mobility of Daphnia magna:
Nominal Concentrations [mg/L] |
Number of Daphnia tested/concentration |
Number of Daphnia immobilized after 24 and 48 hours (as %) |
|
24 h |
48 h |
||
Control |
20 |
0 (0%) |
0 (0%) |
0.1 |
20 |
0 (0%) |
0 (0%) |
0.22 |
20 |
0 (0%) |
0 (0%) |
0.46 |
20 |
0 (0%) |
20 (100%) |
1.0 |
20 |
20 (100%) |
20 (100%) |
2.2 |
20 |
20 (100%) |
20 (100%) |
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.