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EC number: 220-912-4 | CAS number: 2935-90-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1999
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP study conducted according to OECD Guideline 202.
Cross-referenceopen allclose all
- Reason / purpose for cross-reference:
- reference to same study
- Reason / purpose for cross-reference:
- reference to other study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 999
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- not specified
- Principles of method if other than guideline:
- N/A
- GLP compliance:
- yes
Test material
- Reference substance name:
- Methyl 3-mercaptopropionate
- EC Number:
- 220-912-4
- EC Name:
- Methyl 3-mercaptopropionate
- Cas Number:
- 2935-90-2
- Molecular formula:
- C4H8O2S
- IUPAC Name:
- methyl 3-sulfanylpropanoate
- Details on test material:
- - Name of test material (as cited in study report): methyl 3-mercaptopropionate
- Molecular formula (if other than submission substance): Not applicable
- Molecular weight (if other than submission substance): Not applicable
- Smiles notation (if other than submission substance): Not applicable
- Substance type: active
- Physical state: liquid
- Analytical purity: 99.75%
Constituent 1
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
N/A
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- N/A
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
-Method: A stock solution was prepared before the beginning of the test, by mixing 100 mg of the test substance with 1 liter of dilution water. Stock was then diluted to the desired concentrations.
- Eluate: N/A
- Differential loading: N/A
- Controls: Dilution water only
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): N/A
- Concentration of vehicle in test medium (stock solution and final test solution): N/A
- Evidence of undissolved material (e.g. precipitate, surface film, etc): N/A
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Water flea
- Strain: Straus Clone A
- Source: In house culture
- Age at study initiation (mean and range, SD): <24 h
- Weight at study initiation (mean and range, SD): N/A
- Length at study initiation (length definition, mean, range and SD): N/A
- Valve height at study initiation, for shell deposition study (mean and range, SD): N/A
- Peripheral shell growth removed prior to test initiation: N/A
- Method of breeding: parthanogenic
- Feeding during test
- Food type: N/A
- Amount: N/A
- Frequency: not fed during test
ACCLIMATION
- Acclimation period: N/A
- Acclimation conditions (same as test or not): N/A
- Type and amount of food: N/A
- Feeding frequency: N/A
- Health during acclimation (any mortality observed): N/A
QUARANTINE (wild caught)
- Duration: N/A
- Health/mortality: N/A
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Post exposure observation period:
- N/A
Test conditions
- Hardness:
- No data
- Test temperature:
- 20-21 deg. C
- pH:
- 7.75-7.93
- Dissolved oxygen:
- 7.9-8.4 mg/L. Media aerated to saturation prior to test.
- Salinity:
- N/A
- Nominal and measured concentrations:
- Nominal- Control, 0.06, 0.13, 0.28, 0.62, 1.36, and 3.00 mg/L.
Measured concentrations were provided in Table 1. - Details on test conditions:
- TEST SYSTEM
- Test vessel: 120 mL Erlenmeyer
- Type (delete if not applicable): closed
- Material, size, headspace, fill volume: PTFE and aluminum caps
- Aeration: no
- Type of flow-through (e.g. peristaltic or proportional diluter): N/A
- Renewal rate of test solution (frequency/flow rate): N/A
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- No. of vessels per vehicle control (replicates): N/A
- Biomass loading rate: N/A
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: -Dilution water is prepared in the laboratory using pure water and salts according to ISO 6341.
25 mL/L of the solutions below, aerated up to oxygen saturation:
11.76 g CaCl2, 2 H2O /L ultrapure water
4.93 g MgSO4, 7 H2O /L ultrapure water
2.59 g NaHCO3 /L ultrapure water
0.23 g KCl /L ultrapure water
- Total organic carbon:
- Particulate matter: N/A
- Metals: N/A
- Pesticides: N/A
- Chlorine: N/A
- Alkalinity: N/A
- Ca/mg ratio: 4
- Conductivity: N/A
- Culture medium different from test medium: N/A
- Intervals of water quality measurement: N/A
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: test conducted in the dark
- Light intensity: N/A
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Immobilization, observed at 24 h intervals.
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2
- Justification for using less concentrations than requested by guideline: N/A
- Range finding study
- Test concentrations: N/A
- Results used to determine the conditions for the definitive study:N/A - Reference substance (positive control):
- no
Results and discussion
Effect concentrations
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 0.55 mg/L
- Nominal / measured:
- not specified
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- N/A
- Results with reference substance (positive control):
- N/A
- Reported statistics and error estimates:
- N/A
Any other information on results incl. tables
Table 1. Nominal vs. Measured Concentrations
Nominal Conc. |
Measured concentrations (mg/L) |
||
Initial |
Final |
Final/Initial (%) |
|
0.06 |
NA |
NA |
- |
0.13 |
NA |
NA |
- |
0.28 |
0.279 |
0.258 |
92.47 |
0.62 |
0.604 |
0.590 |
97.68 |
1.36 |
1.275 |
1.236 |
96.94 |
3.00 |
2.763 |
2.719 |
98.41 |
NA-not analyzed
Table 2. Percent Immobilization over Time
Nominal conc. (mg/L) |
% Immobilization |
|
24h |
48h |
|
Control |
5 |
10 |
0.06 |
0 |
5 |
0.13 |
0 |
0 |
0.28 |
20 |
45 |
0.62 |
55 |
85 |
1.36 |
45 |
85 |
3.00 |
35 |
90 |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The 48h EC50 of the test substance to D.magna is 0.55 mg/L.
- Executive summary:
N/A
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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