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EC number: 256-881-9 | CAS number: 50977-10-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2012-02-29 to 2012-03-15
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Guideline study, GLP, complete report and good identification of test substance
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 012
- Report date:
- 2012
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- 3-(isodecyloxy)propylamine
- EC Number:
- 250-056-7
- EC Name:
- 3-(isodecyloxy)propylamine
- Molecular formula:
- C13H29NO
- IUPAC Name:
- 3-(isodecyloxy)propylamine
- Test material form:
- other: Liquid
- Details on test material:
- - Name of test material (as cited in study report): 3-(Isodecyloxy)propylamine
- CAS no 30113-45-2
- Physical state: Liquid, light yellow
- Analytical purity: 95.3 %
- Purity test date: 2012-07-03
- Lot/batch No.: 890000022898
- Expiration date of the lot/batch: 2019-09-22
- Stability under test conditions: Not applicable
- Storage condition of test material: Room temperature, protected from light, in the original container, stored under N2
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The test item was weighed out directly in an Erlenmeyer flask, filled up with 200 mL demieralised water and stirred overnight.
- Eluate: Test medium:
250 mL inoculum
16 mL synthetic waste water
234 mL autoclaved tap water
- Differential loading: 6.56 - 13.1 - 26.2 - 52.5 - 104.9 mg/L (test item)
- Controls: Test medium without test or reference item.
Test organisms
- Test organisms (species):
- activated sludge
- Details on inoculum:
- - Preparation of inoculum for exposure: Non adapted activated sludge from the sewage plant at Hildesheim (Municipal sewage treatment plant of 31137 Hildesheim, Germany)
- Pretreatment: Washed twice with autoclaved tap water and adjusted to a dry weight concentration of 3 g/L +/- 10 %. The sludge was used within 24 h after stirring.
- Dry sludge concentration: 2.73 g/L corresponding to 1.40 g/L suspended solids in the test vessels
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
Test conditions
- Hardness:
- Not determined
- Test temperature:
- 20.6 °C
- pH:
- 7.35 (activated sludge)
7.87 (synthetic waste water) - Salinity:
- None, freshwater
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 1000 mL Erlenmeyer flask, DIN 12380
- Aeration: Permanent
- No. of vessels per concentration (replicates): 5
- No. of vessels per control (replicates): 3
- Composition of test medium:
250 mL inoculum
16 mL synthetic waste water
234 mL autoclaved tap water
Test or reference item concentration (directly weighed into each test vessel)
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
After 3 h incubation, the oxygen concentration of each control. reference item and test item replicate was determined by means of oximeter immediately after transferring the solution to the measuring cell and the oxygen depletion was recorded for at least 3 minutes on a flat bed recorder.
TEST CONCENTRATIONS
- Range finding study
Inhibition of Respiration (Preliminary Test)
Nominal Test Item Concentration Pretreatment pH Inhibition [%]
[mg/L]
10 Stirring overnight 8.22 0
100 8.74 89
1000 10.79 95
1000 10.26 97
1000 7.85* 93 - Reference substance (positive control):
- yes
- Remarks:
- Copper (II) sulphate pentahydrate p.a.
Results and discussion
Effect concentrations
- Duration:
- 3 h
- Dose descriptor:
- EC10
- Effect conc.:
- 13 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Remarks on result:
- other: (11.6-14.6)
- Details on results:
- - Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: None
- Results with reference substance (positive control):
- - Results with reference substance valid? Yes
- Relevant effect levels: EC50: 100 mg/L - Reported statistics and error estimates:
- The NOEC was determined by calculation of statistical significance of the inhibition of respiration in comparison to the control. One Way Analysis of Variance (ANOVA) and DUNNETT’s test was used for NOEC calculations. When running a One Way Analysis of Variance a Normality test and an Equal Variance test was done first. P-values for both Normality and Equal Variance test are 0.05. The a-value (acceptable probability of incorrectly concluding that there is a difference) is a=0.05.
The EC-values of the test item were calculated by sigmoidal dose-response regression and the EC50-value of the reference item by linear regression using software GraphPad Prism, GRAPHPAD SOFTWARE, INC. Calculation of the confidence intervals for the EC50-values were carried out using standard procedures.
Any other information on results incl. tables
Oxygen Uptake Rates, Specific Respiration Rates and Inhibition of the Respiration of the Control and Reference Item Concentrations
Reference Item Concentration |
|
Oxygen Uptake Rate |
Specific Respiration Rate |
|
Mean Inhibition |
Control |
1 |
35.2 |
25.1 |
– |
– |
2 |
36.4 |
26.0 |
|||
3 |
37.2 |
26.6 |
|||
4 |
32.0 |
22.9 |
|||
5 |
32.0 |
22.9 |
|||
6 |
31.2 |
22.3 |
|||
58 |
1 |
24.8 |
27 |
27 |
|
2 |
24.4 |
28 |
|||
3 |
25.6 |
25 |
|||
100 |
1 |
17.2 |
49 |
49 |
|
2 |
17.2 |
49 |
|||
3 |
17.2 |
49 |
|||
180 |
1 |
7.6 |
78 |
76 |
|
2 |
8.0 |
76 |
|||
3 |
8.4 |
75 |
Oxygen Uptake Rates and Inhibition of the Respiration of the Test Item Concentrations
|
|
Oxygen Uptake Rate |
|
Mean Inhibition |
6.25 |
1 |
32.0 |
6 |
0 |
2 |
33.6 |
1 |
||
3 |
33.6 |
1 |
||
4 |
36.8 |
-8 |
||
5 |
33.2 |
2 |
||
12.5 |
1 |
30.0 |
12 |
8 |
2 |
33.6 |
1 |
||
3 |
28.8 |
15 |
||
4 |
32.4 |
5 |
||
5 |
32.0 |
6 |
||
25 |
1 |
18.0 |
47 |
56 |
2 |
13.6 |
60 |
||
3 |
13.2 |
61 |
||
4 |
14.4 |
58 |
||
5 |
16.4 |
52 |
||
50 |
1 |
5.6 |
84 |
84 |
2 |
5.2 |
85 |
||
3 |
6.0 |
82 |
||
4 |
6.0 |
82 |
||
5 |
4.8 |
86 |
||
100 |
1 |
3.2 |
91 |
91 |
2 |
2.8 |
92 |
||
3 |
3.2 |
91 |
||
4 |
2.8 |
92 |
||
5 |
3.2 |
91 |
EC-Values with Confidence Interval of 3-(Isodecyloxy)propylamine
EC-values |
Confidence interval |
|
[mg a.i./L] |
P = 95 [%] [mg a.i./L] |
|
NOEC |
12.5 |
– |
EC10 |
13.0 |
11.6 – 14.6 |
EC20 |
16.0 |
14.6 – 17.4 |
EC50 |
23.6 |
22.4 – 24.8 |
EC80 |
38.7 |
34.7 – 43.7 |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The NOEC of the test item 3-(Isodecyloxy)propylamine is 12.5 mg/L. The EC10 and EC50 are resp. 13 and 23.6 mg/L
The test item is not toxic at concentrations = 12.5 mg/L (a.i.) to activated sludge of a municipal sewage treatment plant. - Executive summary:
A Respiration Inhibition Test with activated sludge according to OECD Guideline No. 209 was carried out with the test item 3-(Isodecyloxy)propylamine (batch number: 890000022898) from 2012-02-29 to 2012-03-15, with the experimental phase on 2012-03-15 at Dr.U.Noack-Laboratorien, D-31157 Sarstedt. Test system was activated sludge of the municipal treatment plant of D-31137 Hildesheim. The test was carried out under static conditions with the nominal active ingredient concentrations 6.25 – 12.5 – 25.0 – 50.0 – 100 mg/L. The respiration rates of the control, reference and test item replicates were measured after a contact time of three hours, and the inhibitory effects of the test and reference item were determined in comparison to the control respiration rates. The mean inhibition of respiration for the test item replicates ranged from 0 % to 91 % .
In order to check the activity of the test system and the test conditions a reference test was carried out with copper (II) sulphate pentahydrate as reference item and the reference toxicity was determined. The EC50-value for the reference item was 100 mg/L.
EC-Values with Confidence Interval of 3-(Isodecyloxy)propylamine
EC-values
Confidence interval
[mg a.i./L]
P = 95 [%]
[mg a.i./L]
NOEC
12.5
–
EC10
13.0
11.6 – 14.6
EC20
16.0
14.6 – 17.4
EC50
23.6
22.4 – 24.8
EC80
38.7
34.7 – 43.7
The EC10 of the test item 3 -(Isodecyloxy)propylamine is 13 mg a.i./L.
The test item is not toxic at concentrations </= 12.5 mg a.i./L
to activated sludge of a municipal sewage treatment plant.
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