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EC number: 902-591-9 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
- Qualifier:
- according to guideline
- Guideline:
- other: Standard Methods for the Examination of Water and Wastewater. 1971. 13th Ed. American Public Health Association, New York, NY
- Principles of method if other than guideline:
- closed bottle test (BOD of THOD)
- GLP compliance:
- no
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- sewage, domestic, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): domestic wastewater
- Preparation of inoculum for exposure: Settled domestic wastewater was filtered through glass wool and then added (3 mL/bottle) as seed material to clean 300 mL BOD bottles. The bottles were half filled with aerated dilution water containing specified minerals along with buffer. - Duration of test (contact time):
- 20 d
- Initial conc.:
- 3 mg/L
- Based on:
- test mat.
- Initial conc.:
- 7 mg/L
- Based on:
- test mat.
- Initial conc.:
- 10 mg/L
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- TEST CONDITIONS
- Aeration of dilution water: aerated dilution water used at beginning of test; reaeration when DO of test flasks dropped below 4.0 mg/L
TEST SYSTEM
- Method used to create aerobic conditions: A clean, empty BOD bottle and the test bottle were connected with an adapter; by allowing half of the liquid to pass into the new bottle, followed by moderate shaking, the DO level could be returned to about 7 mg/L. Once this reaeration was accomplished, all of the liquid was returned to the original bottle, and a new DO value was determined.
- Measuring equipment: commercial DO meter
- Test performed in closed vessels due to significant volatility of test substance: yes; flask were only opened in order to reaerate samples
SAMPLING
- Sampling frequency: five times during the 20 day test period - Reference substance:
- not specified
- Parameter:
- % degradation (O2 consumption)
- Value:
- 76
- Sampling time:
- 5 d
- Parameter:
- % degradation (O2 consumption)
- Value:
- 88
- Sampling time:
- 10 d
- Parameter:
- % degradation (O2 consumption)
- Value:
- 91
- Sampling time:
- 15 d
- Key result
- Parameter:
- % degradation (O2 consumption)
- Value:
- 95
- Sampling time:
- 20 d
- Validity criteria fulfilled:
- not specified
- Interpretation of results:
- readily biodegradable
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
- Qualifier:
- according to guideline
- Guideline:
- other: Standard Methods for the Examination of Water and Wastewater. 1971. 13th Ed. American Public Health Association, New York, NY
- Principles of method if other than guideline:
- closed bottle test (BOD of THOD)
- GLP compliance:
- no
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- other: marine water, raw wastewater, bacteria, growth factors
- Details on inoculum:
- - Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure):seawater taken from Lavaca Bay, Texas, USA
- Preparation of inoculum for exposure: Seawater seed source was maintained by adding small amounts of settled raw wastewater about every 3 to 4 days as a source of substrate, seed bacteria, and growth factors. - Duration of test (contact time):
- 20 d
- Initial conc.:
- 3 mg/L
- Based on:
- test mat.
- Initial conc.:
- 7 mg/L
- Based on:
- test mat.
- Initial conc.:
- 10 mg/L
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- TEST CONDITIONS
- Aeration of dilution water: aerated dilution water used at beginning of test; reaeration when DO of test flasks dropped below 4.0 mg/L
TEST SYSTEM
- Method used to create aerobic conditions: A clean, empty BOD bottle and the test bottle were connected with an adapter; by allowing half of the liquid to pass into the new bottle, followed by moderate shaking, the DO level could be returned to about 7 mg/L. Once this reaeration was accomplished, all of the liquid was returned to the original bottle, and a new DO value was determined.
- Measuring equipment: commercial DO meter
- Test performed in closed vessels due to significant volatility of test substance: yes; flask were only opened in order to reaerate samples
SAMPLING
- Sampling frequency: five times during the 20 day test period - Reference substance:
- not specified
- Parameter:
- % degradation (O2 consumption)
- Value:
- 69
- Sampling time:
- 5 d
- Parameter:
- % degradation (O2 consumption)
- Value:
- 84
- Sampling time:
- 10 d
- Parameter:
- % degradation (O2 consumption)
- Value:
- 85
- Sampling time:
- 15 d
- Key result
- Parameter:
- % degradation (O2 consumption)
- Value:
- 97
- Sampling time:
- 20 d
- Interpretation of results:
- readily biodegradable
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- Respirometric test (BOD of THOD). Determination of the reaction kinetic by means of the respirometric dilution method. This method involves the use of a nutritious matrix, usually but not necessarily domestic sewage, which contains a lot of organic substances competing as nutrient with the substrate under test.
- GLP compliance:
- no
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- sewage, domestic, non-adapted
- Details on inoculum:
- Sewage water from a domestic sewage treatment plant in Stuttgart, Germany was sieved and if necessary diluted with tap water to reach a consumption of potassium permanganate of 250 - 300 mg/L
- Duration of test (contact time):
- 8 - 10 d
- Initial conc.:
- 4 - 200 g/L
- Based on:
- other: test material along with Urea and Di-sodiumhydrogenphosphat-12-hydrate
- Parameter followed for biodegradation estimation:
- O2 consumption
- Reference substance:
- not specified
- Key result
- Parameter:
- % degradation (O2 consumption)
- Remarks:
- BOD/ThOD
- Value:
- 82.7
- Sampling time:
- 5 d
- Details on results:
- ThOD: 1498 mg/g
rate constant K: 1.882 1/d
L/ThOD at day 5: 82.5% - Key result
- Parameter:
- BOD5
- Value:
- 1 236 mg O2/g test mat.
- Validity criteria fulfilled:
- not applicable
- Interpretation of results:
- readily biodegradable
- Conclusions:
- The test item was found to be readily biodgradabale after (82.7% after 5 days based on BOD/ThOD).
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- Respirometric test (BOD of THOD). Determination of the reaction kinetic by means of the respirometric dilution method. This method involves the use of a nutritious matrix, usually but not necessarily domestic sewage, which contains a lot of organic substances competing as nutrient with the substrate under test.
- GLP compliance:
- no
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- other: other bacteria: synthetic sewage
- Details on inoculum:
- - Preparation of inoculum for exposure:
synthetic waste water: the following components were diluted in 1L tap water: 156.3 mg Peptone from Casein, 104.2 meat extract, 27.1 mg Urea, 7.8 mg Penta-sodiumtriphosphate, 4.2 mg Calciumchloride, 2.1 mg Magnesium sulphate, 6.2 mg Sodiumchloride.
1L of sieved and adapted water from a domestic sewage treatment plant in Stuttgart, Germany was centrifuged for 30 min. After decantation the residuum was diluted again in 100 mL tap water, shaken and centrifuged. The resulting sediment was then diluted in 1L of the synthetic wastewater. After stirring for 3 - 4 hours the mixture was used for the preparation of the dilution series. - Duration of test (contact time):
- 8 - 10 d
- Initial conc.:
- 4 - 200 g/L
- Based on:
- other: test material along with Urea and Di-sodiumhydrogenphosphat-12-hydrate
- Parameter followed for biodegradation estimation:
- O2 consumption
- Reference substance:
- not specified
- Preliminary study:
- no data
- Key result
- Parameter:
- other: BOD/ThOD
- Value:
- 71.5
- Sampling time:
- 5 d
- Details on results:
- ThOD: 1498 mg/g
rate constant K: 1.397 1/d
L/ThOD at day 5: 71.2% - Key result
- Parameter:
- BOD5
- Value:
- 1 067 mg O2/g test mat.
- Validity criteria fulfilled:
- not applicable
- Interpretation of results:
- readily biodegradable
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- weight of evidence
- Justification for type of information:
- Please refer to IUCLID section 13 for Read Across Justification.
- Reason / purpose for cross-reference:
- read-across source
- Key result
- Parameter:
- % degradation (O2 consumption)
- Value:
- 97
- Sampling time:
- 20 d
- Interpretation of results:
- readily biodegradable
- Endpoint:
- biodegradation in water: inherent biodegradability
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- weight of evidence
- Justification for type of information:
- Please refer to IUCLID section 13 for Read Across Justification.
- Reason / purpose for cross-reference:
- read-across source
- Key result
- Parameter:
- % degradation (DOC removal)
- Value:
- 30
- Sampling time:
- 3 h
- Conclusions:
- In a Zahln-Wellens test the test item was degradated ca. 30 % after 3 h (36 % or 44 %, resp.) refered to DOC and >95 % after 6 d.
- Endpoint:
- biodegradation in water: inherent biodegradability
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- weight of evidence
- Justification for type of information:
- Please refer to IUCLID section 13 for Read Across Justification.
- Reason / purpose for cross-reference:
- read-across source
- Key result
- Parameter:
- % degradation (O2 consumption)
- Remarks:
- BOD
- Value:
- 92
- Sampling time:
- 14 d
- Interpretation of results:
- readily biodegradable
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- weight of evidence
- Justification for type of information:
- Please refer to IUCLID section 13 for Read Across Justification.
- Reason / purpose for cross-reference:
- read-across source
- Key result
- Parameter:
- % degradation (O2 consumption)
- Value:
- 70
- Sampling time:
- 28 d
- Interpretation of results:
- readily biodegradable
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- weight of evidence
- Justification for type of information:
- Please refer to IUCLID section 13 for Read Across Justification.
- Reason / purpose for cross-reference:
- read-across source
- Key result
- Parameter:
- % degradation (O2 consumption)
- Value:
- 95
- Sampling time:
- 20 d
- Interpretation of results:
- readily biodegradable
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- weight of evidence
- Justification for type of information:
- Please refer to IUCLID section 13 for Read Across Justification.
- Reason / purpose for cross-reference:
- read-across source
- Key result
- Parameter:
- % degradation (O2 consumption)
- Remarks:
- BOD/ThOD
- Value:
- 82.7
- Sampling time:
- 5 d
- Interpretation of results:
- readily biodegradable
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- weight of evidence
- Justification for type of information:
- Please refer to IUCLID section 13 for Read Across Justification.
- Reason / purpose for cross-reference:
- read-across source
- Key result
- Parameter:
- % degradation (O2 consumption)
- Remarks:
- BOD/DOC
- Value:
- 71.5
- Sampling time:
- 5 d
- Interpretation of results:
- readily biodegradable
- Endpoint:
- biodegradation in water: inherent biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Reliability:
- 4 (not assignable)
- Rationale for reliability incl. deficiencies:
- documentation insufficient for assessment
- Remarks:
- study by reputated testing insitute according to common laboratory guidelines
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 302 B (Inherent biodegradability: Zahn-Wellens/EMPA Test)
- Principles of method if other than guideline:
- not specified
- GLP compliance:
- no
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, industrial (adaptation not specified)
- Key result
- Parameter:
- % degradation (DOC removal)
- Value:
- 30
- Sampling time:
- 3 h
- Conclusions:
- In a Zahln-Wellens test the test item was degradated ca. 30 % after 3 h (36 % or 44 %, resp.) refered to DOC and >95 % after 6 d.
- Executive summary:
To assess the biodegradability of the test item a Zahn-Wellens test was performed without controls. The elimination was presumably by stripping to some extent. As a result, the test item was eliminated 30 % after 3 h (36 % or 44 %, resp.) based on DOC. An eliminiation of >95 % was detected after after 6 d. The quota of abiotic elimination is not quantifiable.
- Endpoint:
- biodegradation in water: inherent biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Remarks:
- study published by a recognized Japanese authority
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 C (Ready Biodegradability: Modified MITI Test (I))
- GLP compliance:
- not specified
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- other: see "Details on inoculum"
- Details on inoculum:
- - Source of inoculum/activated sludge: Ten sampling sites for domestic and industrial sludge as well as rivers are stated in the report, because the document deal with more then one test on biodegradability. It is assumed that non-adapted domestic sludge was used, because test on ready (and not inherent) biodegradability was conducted.
- Laboratory culture: The filtrate of the supernatant of an activated sludge in present use was mixed with the filtrate of the supernatant of a newly collected sludge. The mixture was cultured at pH 7.0 +/- 1.0 under sufficient aeration. About 30 minutes after stopping the aeration, about 1/3 of the volume of the supenatant was removed. An equal volume of dechlorinated water was added and aerated again and synthetic sewage was added at an amount of 0.1 % (w/v). This procedure was repeated every day. The culturing was out at 25° +/- 2°C. - Duration of test (contact time):
- 14 d
- Initial conc.:
- 100 mg/L
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- TEST CONDITIONS
- Composition of medium: Basal medium according to JIS 102-1986-14.1
- Test temperature: 25 +/- 1°C
- pH: pH of the basal culture medium was adjusted to 7.0. During the test, pH of each test solution was measured, but detailes are not specified.
- pH adjusted: yes (basal culture medium)
- Suspended solids concentration: 30 mg/L
TEST SYSTEM
- Culturing apparatus: 300 ml test vessels
- Measuring equipment: Closed system oxygen consumption measuring apparatus (Ohkura Electric Co., Ltd)
- Test performed in closed vessels due to significant volatility of test substance: Yes. It is stated in the report that an "improved type for a volatile substance" was used
- Details of trap for CO2 and volatile organics if used: Soda lime was used for the absorption of carbon dioxide
- Other: Test solution was stirred by a magnetic stirrer.
CONTROL AND BLANK SYSTEM
- Inoculum blank: Yes (basal culture medium only)
- Abiotic sterile control: Yes (purified water and test substance)
- Toxicity control: No - Reference substance:
- aniline
- Key result
- Parameter:
- % degradation (O2 consumption)
- Remarks:
- BOD
- Value:
- 92
- Sampling time:
- 14 d
- Remarks on result:
- other: Indirect analysis
- Parameter:
- % degradation (TOC removal)
- Value:
- 96
- Sampling time:
- 14 d
- Remarks on result:
- other: Direct analysis
- Parameter:
- % degradation (test mat. analysis)
- Value:
- 100
- Sampling time:
- 14 d
- Remarks on result:
- other: Direct analysis (GC determination)
- Results with reference substance:
- not stated
- Validity criteria fulfilled:
- not specified
- Interpretation of results:
- readily biodegradable
- Executive summary:
The ready biodegradability of methyl acetate was evaluated in a modified MITI test according to OECD 301 C over a period of 14 days. A solution of 100 ppm test substance was inoculated with activated sludge. Aniline was used as the reference substance. BOD, TOC as well as a gaschromatographical determination of the test substance at the end of the test period served as parameters for degradation. At the end of the test period, all endpoint were beyond at least 92%, indicating a ready biodegradability of the test substance.
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Study period:
- 24.11.1994 - 22.12.1994
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.4-E (Determination of the "Ready" Biodegradability - Closed Bottle Test)
- Qualifier:
- according to guideline
- Guideline:
- other: ISO DIS 10707 (1994)
- GLP compliance:
- yes
- Remarks:
- Data owner has QAU statement
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge: Effluent of the STP of the city of Frankfurt/Main, Frankfurt-Sindlingen
- Preparation of inoculum for exposure: filtration
- Pretreatment: aeration for three days for the depletion of BOD
- Concentration of sludge: 0.5 ml/l
- Type and size of filter used, if any: folded filter. No more details given - Duration of test (contact time):
- 28 d
- Initial conc.:
- 3.6 mg/L
- Based on:
- test mat.
- Initial conc.:
- 5.44 mg/L
- Based on:
- COD
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- TEST CONDITIONS
- Test temperature: 20°C +/- 1°C
- pH: 7.37 (stock solution)
- pH adjusted: yes, the pH of the stock solution was adjusted with NaOH from 6.98 to 7.37
- Test performed in closed vessels due to significant volatility of test substance: yes (closed bottle test according to guideline)
- Test performed in open system: no
TEST SYSTEM
- Culturing apparatus: 250 ml BOD flasks
- Number of culture flasks/concentration: 3
SAMPLING
- Sampling frequency: on day 0, 7, 14, 19 and 28
CONTROL AND BLANK SYSTEM
- Inoculum blank: yes
- Abiotic sterile control: no
- Toxicity control: no - Reference substance:
- other: sodium benzoate
- Parameter:
- % degradation (O2 consumption)
- Value:
- 1
- Sampling time:
- 0 d
- Key result
- Parameter:
- % degradation (O2 consumption)
- Value:
- 70
- Sampling time:
- 28 d
- Parameter:
- % degradation (O2 consumption)
- Value:
- 52
- Sampling time:
- 7 d
- Parameter:
- % degradation (O2 consumption)
- Value:
- 74
- Sampling time:
- 14 d
- Parameter:
- % degradation (O2 consumption)
- Value:
- 75
- Sampling time:
- 19 d
- Details on results:
- After a lag phase of two days, the degradation of methyl acetate based on oxygen consumption reached > 68% within 10 days. Within the test period of 28 days, the degradation of methyl acetate reached 70%. The oxygen consuption based on endogenic respiration of the bacteria was determined in the inoculum blank and remained below 1 mg/L O2
- Results with reference substance:
- The degradation rate of the reference substance sodium benzoate was > 60% of its theoretical maximum within 14 days.
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- readily biodegradable
- Conclusions:
- After a lag-phase of two days, degradation of methyl acetate reached > 68 % within the ten-day window and 70 % within the complete test duration of 28 days. The reference substance sodium benzoate reached a degradation of 88 % within 14 days, indicating the suitability of the test system. Based on the results, mehtyl acetate can be classified as "ready biodegradabel, reaching the ten-day window".
- Executive summary:
The ready biodegradability of methyl acetate was tested in a closed bottle test accoding to OECD 301 D over a period of 28 days. Sodium benzoate served as the reference substance. The mean degradation rate of methyl acetate based on oxygen was > 68% within the 10-day window and reached 70% at the end of the test period. Methyl acetate is therefore considered to be ready biodegradable reaching the 10-days window.
Referenceopen allclose all
Table 1: Mean degradation rates of methyl acetate and the reference substance sodium benzoate based on oxygen consumption
|
Degradation rate [%] |
|
Day |
Methyl acetate |
Sodium benzoate |
0 |
1 |
0 |
7 |
52 |
66 |
14 |
74 |
88 |
19 |
75 |
94 |
28 |
70 |
71 |
Description of key information
Based on a read across to the source substances Methanol and Methyl acetate the registration substance is considered to be readily biodegradable.
Key value for chemical safety assessment
- Biodegradation in water:
- readily biodegradable
- Type of water:
- freshwater
Additional information
No data on the biodegradability of the target substance are available. Therefore, a read across with structural analogues Methanol and Methyl acetate was applied. The available information was assessed in a weight of evidence approach.
Methanol
Methanol is readily biodegradable in freshwater based on the results of standard ready biodegradability tests that show 71.5 – 95 percent removal after 5 and 20 days, respectively (Price et al. 1974; Wagner 1976). In marine water degradation rates were found between 69 - 97 % (Price et al. 1974).
Methyl acetate
The ready biodegradability of methyl acetate was tested in a closed bottle test according to OECD 301 D over a period of 28 days. Sodium benzoate served as the reference substance. The mean degradation rate of methyl acetate based on oxygen was > 68% within the 10-day window and reached 70% at the end of the test period. Methyl acetate is therefore considered to be ready biodegradable reaching the 10-days window.
The ready biodegradability of methyl acetate was evaluated in a modified MITI test according to OECD 301 C over a period of 14 days by the National Institute of Technology and Evaluation, Japan (1993). A solution of 100 ppm test substance was inoculated with activated sludge. Aniline was used as the reference substance. BOD, TOC as well as a gaschromatographical determination of the test substance at the end of the test period served as parameters for degradation. At the end of the test period, all endpoints were beyond at least 92%, indicating a ready biodegradability of the test substance.
In a Zahn-Wellens test methyl acetate was assessed for biodegradability without control system. The elimination was presumably by stripping to some extent. As a result, the test item was eliminated 30 % after 3 h (36 % or 44 %, resp.) based on DOC. An eliminiation of >95 % was detected after after 6 d. The quota of abiotic elimination is not quantifiable (Hoechst, 1985). The study is considered not assignable (Klimisch 4) because of insufficient documentation and is not further considered for assessment.
Conclusion
Methanol was found to be readily degradable under both aerobic and anaerobic conditions in a wide variety of environmental media including fresh and salt water, sediments and soils, ground water, aquifer material and industrial wastewater. These findings are in line with results on the degradability of Methyl acetate: the ready biodegradability of methyl acetate has been proven to be readily biodegradable in a closed bottle test according to OECD 301 D and a modified MITI test according to OECD 301 C. In conclusion, based on reliable experimental data for both source substances and by applying a read across approach in compliance with section 1 of REACH Annex XI the target substance is considered to be readily biodegradable.
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