2-aminoethyldiethylamine

Eye irritation

Administrative data

Endpoint:

eye irritation: in vivo

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1969

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Scientifically acceptable study report.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1969

Report date:

1969

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 405 (Acute Eye Irritation / Corrosion)

Deviations:

not specified

Principles of method if other than guideline:

According to BASF-internal standard, see details in remarks on material and methods.

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

2-aminoethyldiethylamine

EC Number:

202-844-7

EC Name:

2-aminoethyldiethylamine

Cas Number:

100-36-7

Molecular formula:

C6H16N2

IUPAC Name:

N1,N1-Diethyl-1,2-ethanediamine

Details on test material:

Name of the test substance used in the study report: N,N-Diaethyl-aethylendiamin

Test animals / tissue source

Species:

rabbit

Strain:

Vienna White

Test system

Vehicle:

unchanged (no vehicle)

Controls:

other: he adjacent eye served as saline control.

Amount / concentration applied:

TEST MATERIAL
- Amount applied: 50 µL

Duration of treatment / exposure:

Single application

Observation period (in vivo):

8 days

Number of animals or in vitro replicates:

2

Details on study design:

50 µL of the test substance were applied to the conjunctival sac of one eye in 2 animals. The animals were observed after 10 min, 1 and 3 hours on the day of treatment and up to 8 days afterwards. The eyes were not washed out after 24 hours as specified in OECD Guideline 405. The report describes findings after 1 and 24 hours and at the end of the observation period.

Results and discussion

In vivo

Results

Irritation parameter:

other: corrosion

Basis:

mean

Time point:

other: within few minutes

Reversibility:

not fully reversible within: 8 days

Remarks on result:

other: Reaction observed shortly after instillation.

Any other information on results incl. tables

Already after a few minutes of the test substance instillation, corrosion of the eyes was observed.

Applicant's summary and conclusion

Interpretation of results:

Category 1 (irreversible effects on the eye) based on GHS criteria

Executive summary:

In this acute eye irritation/corrosion study (BASF, 1969), the eyes of 2 Vienna White rabbits were exposed by application of 50 µL undiluted test substance into the conjunctival sac. The animals were observed after 10 min, 1 and 3 hours on the day of treatment and up to 8 days afterwards. The eyes were not washed out after 24 hours. Overall, the treatment caused corrosion of the eye mucous membranes within 1 hour.Thus, the test substance was graded as corrosive under the conditions of this study. This study is classified as acceptable. It satisfies the requirements of OECD test guideline 405.