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EC number: 226-603-0 | CAS number: 5435-64-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Eye irritation
Administrative data
- Endpoint:
- eye irritation: in vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- October 1996 until April 1997
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 997
- Report date:
- 1997
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 405 (Acute Eye Irritation / Corrosion)
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- 3,5,5-trimethylhexanal
- EC Number:
- 226-603-0
- EC Name:
- 3,5,5-trimethylhexanal
- Cas Number:
- 5435-64-3
- Molecular formula:
- C9H18O
- IUPAC Name:
- 3,5,5-trimethylhexanal
- Details on test material:
- klare, farblose Flüssigkeit
Flüssigkeit erscheint optisch homogen
ph-Wert nicht messbar
Haltbarkeit > 1 Jahr
Reinheit 91,2 mass-% (Gas-Chromatographie)
Constituent 1
Test animals / tissue source
- Species:
- rabbit
- Strain:
- Himalayan
- Remarks:
- Mol: Russian/Chbb: HM
- Details on test animals or tissues and environmental conditions:
- TEST ANIMALS
- Source: Mollegard Breeding & Research Centre Ltd., Skensved, Denmark
- Age at study initiation: adult
- Weight at study initiation:2,3 - 2,6 kg
- Housing: conventional, single-housed
- Diet: ad libitum
- Water : ad libitum
- Acclimation period: at least 14 days
-sex: male
ENVIRONMENTAL CONDITIONS
- Temperature (°C):20 +/- 3 ° C
- Humidity (%):30 - 70 %
- Air changes (per hr): 15-fold/hr
- Photoperiod (hrs dark / hrs light): 12/12
Test system
- Vehicle:
- unchanged (no vehicle)
- Controls:
- no
- Amount / concentration applied:
- single administration of 0.1 cm³ of the undiluted liquid
the liquid test substance was placed in the conjunctival sac of an eye - Duration of treatment / exposure:
- 24 hr
- Observation period (in vivo):
- 24 h, 48 h, 72 h,
6, 8, 10 and 13 days after application of the test item - Number of animals or in vitro replicates:
- 3
- Details on study design:
- One day before the application of the test substance, the animals were examined for their general state of health. In addition, the eyes of the animals were exposed to binding eye damage and after instillation of a drop of fluorescein sodium solution (0.1%) by means of an eye lamp
possible damage to the cornea.
The liquid test substance (application volume: 0.1 cm.sup.3) was first dripped into the conjunctival sac of an eye only once in one animal because of the strong skin irritation effect of the test substance. The untreated eye served as a control. Because only light eye and eye mucosa stimuli were observed in this rabbit, a week later 2 animals were under the same test conditions aftertreated.
24 hours post-application, the treated eyes were examined in all animals after the fluorescein test with warm physiological saline-
solution. In animals 1, 2, and 3, the treated eye was treated 24, 48, and 72 hours, respectively, p.a. as well as 6 days p.a. (Animals 2 and 3) according to the assessment with warm physical NaCl. In the reacted animals, the fluorescein test 24, 48, and 72 h p.a. as well as after 6 days
carried out.
TOOL USED TO ASSESS SCORE: fluorescein
Results and discussion
In vivo
Resultsopen allclose all
- Irritation parameter:
- cornea opacity score
- Basis:
- mean
- Time point:
- 24/48/72 h
- Score:
- 0.33
- Max. score:
- 1
- Reversibility:
- fully reversible within: 6 days
- Irritation parameter:
- cornea opacity score
- Basis:
- animal #3
- Time point:
- 24/48/72 h
- Score:
- 1
- Max. score:
- 1
- Reversibility:
- fully reversible within: 6d
- Irritation parameter:
- cornea opacity score
- Basis:
- animal #2
- Time point:
- 24/48/72 h
- Score:
- 0
- Reversibility:
- other: no effect
- Irritation parameter:
- cornea opacity score
- Basis:
- animal #1
- Time point:
- 24/48/72 h
- Score:
- 0
- Reversibility:
- other: no effect
- Irritation parameter:
- iris score
- Basis:
- mean
- Time point:
- 24/48/72 h
- Score:
- 0.11
- Max. score:
- 0.33
- Reversibility:
- fully reversible within: 48 hours
- Irritation parameter:
- iris score
- Basis:
- animal #3
- Time point:
- 24/48/72 h
- Score:
- 0.33
- Max. score:
- 1
- Reversibility:
- fully reversible within: 48h
- Irritation parameter:
- iris score
- Basis:
- animal #2
- Time point:
- 24/48/72 h
- Score:
- 0
- Reversibility:
- other: no effect
- Irritation parameter:
- iris score
- Basis:
- animal #1
- Time point:
- 24/48/72 h
- Score:
- 0
- Reversibility:
- other: no effect
- Irritation parameter:
- chemosis score
- Basis:
- mean
- Time point:
- 24/48/72 h
- Score:
- 1.11
- Max. score:
- 1
- Reversibility:
- fully reversible within: 8 days
- Irritation parameter:
- chemosis score
- Basis:
- animal #3
- Time point:
- 24/48/72 h
- Score:
- 1
- Max. score:
- 1
- Reversibility:
- fully reversible within: 8d
- Irritation parameter:
- chemosis score
- Basis:
- animal #2
- Time point:
- 24/48/72 h
- Score:
- 1
- Max. score:
- 1
- Reversibility:
- fully reversible within: 6d
- Irritation parameter:
- chemosis score
- Basis:
- animal #1
- Time point:
- 24/48/72 h
- Score:
- 1
- Max. score:
- 1
- Reversibility:
- fully reversible within: 6d
- Irritation parameter:
- conjunctivae score
- Basis:
- animal #3
- Time point:
- 24/48/72 h
- Score:
- 2
- Max. score:
- 2
- Reversibility:
- fully reversible within: 13d
- Irritation parameter:
- conjunctivae score
- Basis:
- animal #2
- Time point:
- 24/48/72 h
- Score:
- 1.33
- Max. score:
- 2
- Reversibility:
- fully reversible within: 8d
- Irritation parameter:
- conjunctivae score
- Basis:
- mean
- Time point:
- 24/48/72 h
- Score:
- 1.55
- Max. score:
- 2
- Reversibility:
- fully reversible within: 13 days
- Irritation parameter:
- conjunctivae score
- Basis:
- animal #1
- Time point:
- 24/48/72 h
- Score:
- 1.33
- Max. score:
- 2
- Reversibility:
- fully reversible within: 6d
- Irritant / corrosive response data:
- see excecutive summary
Applicant's summary and conclusion
- Interpretation of results:
- GHS criteria not met
- Remarks:
- Criteria used for interpretation of results: GHS
- Conclusions:
- Not to be classifed as eye irritating accordingt to CLP Regulation (EC) No. 1272/2008
- Executive summary:
For the test for acute eye and mucous membrane stimulation of TRIMETHYLHEXANAL, three rabbits were administered each 0.1 cm.sup.3 of the liquid test substance into the conjunctival sac of an eye. After an exposure time of 24 hours, the eyes were rinsed with warm physiological saline solution.
24 hours postapplication (p.a.) of the test substance, reactions were found in all three animals in the form of diffuse, crimson discoloration, so that individual vessels are difficult to recognize. Associated with this was a swelling over the normal, with partial removal of the lids (1 animal). The cornea was slightly opaque in an animal between 1/2 and 3/4 of the surface. The iris showed a circumcorneal hyperemia. At this observation time, 2 animals were without corneal and iris changes. 24 to 48 hours p.a. was observed in an animal eye secretion. 48 and 72 hours p.a. these eye and mucous membrane stimuli were observed in all three animals in a weakened form. The circumcorneal hyperemia on the iris occurred in an animal for 48 h. already no longer observed. The slight corneal opacity in an animal was no longer present on day 6 of the experiment. On this day was already an animal, on the 8th day were two animals and on the 13th day all three animals without eyesight.
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