Registration Dossier
Registration Dossier
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EC number: 435-990-7 | CAS number: 371921-34-5
Toxicity to microorganisms
Administrative data
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- From March 26 to March 29, 2001
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 001
- Report date:
- 2001
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Version / remarks:
- 1984
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.11 (Biodegradation: Activated Sludge Respiration Inhibition Test)
- Version / remarks:
- 1988
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Yellow 981
- IUPAC Name:
- Yellow 981
- Test material form:
- solid: particulate/powder
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Vehicle:
- no
Test organisms
- Test organisms (species):
- activated sludge, domestic
Study design
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
Test conditions
- Test temperature:
- 20 °C
- pH:
- 8.0 - 8.6
- Dissolved oxygen:
- 6.7 - 8.4 mg O2/l
- Nominal and measured concentrations:
- - Nominal concentrations: 10, 32, 100, 320 and 1000 mg/l
- Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorephenol (5, 16 and 50 mg/l)
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 3 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Details on results:
- No significant (> 15 %) inhibitory effect on the respiration rate of activated sludge microorganisms at any test concentration.
Up to 1000 mg/l, test item had no significant inhibitory effect (< 15 %) on the respiration rate of activated sludge after the incubation period of 3 hours. Based on the physical appearance of test suspensions. the saturation concentration (equilibrium) under test conditions was between 10 and 32 mg/l and resulted in less than 15 % inhibition after 3 hours contact time. Thus, EC50 is higher than the water solubility limit of test item, or higher then nominal 1000 mg/l; NOECof test item was at least at the limit of water solubility or at least at nominal 1000 mg/l. - Results with reference substance (positive control):
- The 3-hour EC50 of the reference item 3,5-dichlorophenol was calculated to be 29.3 mg/l. The 3-hour EC50 is within the guideline-recommended range of 5-30 mg/l, confirming suitability of the activated sludge used.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- EC(3h) > 1000 mg/l (nominal)
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