Registration Dossier
Registration Dossier
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EC number: 435-990-7 | CAS number: 371921-34-5
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- From October 30 to December 6, 2000
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 001
- Report date:
- 2001
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Version / remarks:
- 1984
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Version / remarks:
- 1992
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Yellow 981
- IUPAC Name:
- Yellow 981
- Test material form:
- solid: particulate/powder
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
Test solutions
- Vehicle:
- no
- Details on test solutions:
- Mixed into the test water as homogeneously as possible by ultrasonic treatment for 15 minutes and by intense stirring for 96 h at room temperature in the dark to dissolve a maximum concentration of the test item in the dispersion.
Test organisms
- Test organisms (species):
- Daphnia magna
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
Test conditions
- Test temperature:
- 20 - 21 °C
- pH:
- 7.8 - 7.9
- Dissolved oxygen:
- 8.0 - 8.7 mg O2/l
- Nominal and measured concentrations:
- - Nominal concentrations: 100 mg/l
- Measured concentrations: day 0 (beginning of test): 0.32 mg/l; day 2 (end of test): 0.41 mg/l; mean: 0.36 mg/l - Reference substance (positive control):
- no
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 48 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: 0.36 mg/l mean measured concentration
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: 0.36 mg/l mean measured concentration
- Details on results:
- Light yellow colouration caused by the test item was apparent throughout the study period. No mortality or symptoms of toxicity observed throughout the study period.
Applicant's summary and conclusion
- Conclusions:
- LC50 (48h) > 100 mg/l (nominal)
NOEC (48h) = 100 mg/l (nominal)
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