Registration Dossier

Data platform availability banner - registered substances factsheets

Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Toxicity to microorganisms

Currently viewing:

Administrative data

Link to relevant study record(s)

Reference
Endpoint:
activated sludge respiration inhibition testing
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1990
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study with acceptable restrictions
Qualifier:
according to guideline
Guideline:
other: EEC L 133, p. 118-122 (30. May 1988 )
GLP compliance:
no
Analytical monitoring:
no
Test organisms (species):
activated sludge, domestic
Details on inoculum:
The preparation was carried out according to the method described in the guideline. The sludge was separated from the aqueous layer by settling instead of centrifugation.
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
3 h
Test temperature:
20 ± 2 °C
Nominal and measured concentrations:
Nominal: 101, 33.5, 11.5, 3.8 and 1.8, 0.0 mg/L
Details on test conditions:
TEST SYSTEM
- Vessel: 250 ml BOD flasks with gas inlet
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 2
- No. of vessels per concentration reference substance (replicates): 1
- Sludge concentration in the test medium: 1.8 g/1.

TEST MEDIUM / WATER PARAMETERS
- Source of dilution water: Dechlorinated drinking water

Reference substance (positive control):
yes
Remarks:
3,5-Dichlorophenole
Duration:
3 h
Dose descriptor:
other: EC20
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Duration:
3 h
Dose descriptor:
EC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Duration:
3 h
Dose descriptor:
other: EC80
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Results with reference substance (positive control):
EC50(3h) = 20.1 mg/L
Validity criteria fulfilled:
not specified
Conclusions:
The 3 hour EC50 of the test material to sewage microorganisms was determined to be >100 mg/l under the conditions of the test.

Description of key information

The study provided was conducted to recognised testing guidelines and with GLP certification.

Key value for chemical safety assessment

Additional information

In a non-GLP-study on inhibition of oxygen consumption by domestic sludge following EEC guideline L 133, p. 118-122 (30. May 1988 ) an EC20 (180 min) of > 100 mg/l was determined [Ciba-Geigy, 1990].