Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 700-292-6 | CAS number: 135043-68-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://echa.europa.eu/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_toxicological-information.png)
Skin irritation / corrosion
Administrative data
- Endpoint:
- skin irritation: in vitro / ex vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Performed to GLP standards
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 008
- Report date:
- 2008
Materials and methods
Test guideline
- Qualifier:
- no guideline available
- Principles of method if other than guideline:
- Evaluating the skin irritation potential of trisodium 6,6',6"-(1,3,5-triazine-2,4,6-triyltriimino)trihexanoate using the EPISKIN reconstituted human epidermis model after a treatment preiod of 15 minutes followed by a post-exposure incubation period of 42 hours. The principle of the assay is to measure cytotoxicity in reconstituted human epidermal cultures following topical exposure to trisodium 6,6',6"-(1,3,5-triazine-2,4,6-triyltriimino)trihexanoate by means of the colourmetric MTT reduction assay.
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- trisodium 6,6',6"-(1,3,5-triazine-2,4,6-triyltriimino)trihexanoate
- IUPAC Name:
- trisodium 6,6',6"-(1,3,5-triazine-2,4,6-triyltriimino)trihexanoate
Constituent 1
Test animals
- Species:
- other: in vitro test
- Strain:
- other: in vitro test
- Details on test animals or test system and environmental conditions:
- Test for direct MTT reduction: 37 C, 5% CO2 in air for 3 hours.
Pre-incubation: 37 C, 5% CO2 in air for at least 24 hours.
Main test: 37 C, 5% CO2 in air for 42 hours.
Test system
- Type of coverage:
- not specified
- Preparation of test site:
- other: moistened with sterile distilled water
- Vehicle:
- unchanged (no vehicle)
- Controls:
- other:
- Amount / concentration applied:
- 10 +/- 2 mg of trisodium 6,6',6"-(1,3,5-triazine-2,4,6-triyltriimino)trihexanoate was applied to the epidermis surface. No vehicle was used. The epidermis surface had previously been moistened with 5 μl of sterile distilled water to improve contact between the solid test material and the epidermis.
- Duration of treatment / exposure:
- 15 minute exposure period
- Number of animals:
- No animals involved as this was an in vitro test.
- Details on study design:
- Pre-test (assessment of direct test material): trisodium 6,6',6"-(1,3,5-triazine-2,4,6-triyltriimino)trihexanoate is checked for the ability to directly reduce MTT. 10 mg of trisodium 6,6',6"-(1,3,5-triazine-2,4,6-triyltriimino)trihexanoate is added to 2 ml of a 0.3 mg/ml MTT solution (v/v) prepared in assay medium. The solution was incubated in the dark at 37 C, 5% CO2 in air for 3 hours. Untreated MTT solution was used as a control. If the MTT solution containing the test substance turns blue/purple, is it considered reduced.
The epidermis tissues were pre-incubated at 37 C, 5% CO2 in air for at least 24 hours before the main test.
Main test: Triplicate tissues were treated with the test material for an exposure period of 15 minutes. 10 +/- 2 mg of trisodium 6,6',6"-(1,3,5-triazine-2,4,6-triyltriimino)trihexanoate was applied to the epidermis surface. No vehicle was used. The epidermis surface had previously been moistened with 5 μl of sterile distilled water to improve contact between the solid test material and the epidermis. Triplicate tissues, treated with PBS are used as negative controls. To ensure satisfactory contact with the positive control material, the SDS solution was spread over the entire surface of the epidermis using a pipette tip. After 7 minutes contact time, the SDS solution was re-spread with a pipette tip to maintain the distribution of the SDS for the remainder fo the contact period. The plates were kept in a biological safety cabinet at room temperature for 15 +/- 0.5 minutes. At the end of the exposure period, each tissue was rinsed with PBS and placed into maintenance medium and incubated at 37 C, 5% CO2 in air for 42 hours. A MTT assay was carried out with the each tissue and the optical density value was measured for each tissue to determine cell viability.
in vitro controls: sodium dodecyl sulphate used as the positive control material; Dulbecco's phosphate buffered saline used as the negative control material.
Results and discussion
In vitro
Results
- Irritation / corrosion parameter:
- other: other: Relative mean % viability
- Remarks on result:
- other:
- Remarks:
- Remarks: in vitro test: see remarks on results section below for scores. (migrated information)
In vivo
- Irritant / corrosive response data:
- For each test material, the relative mean tissue viabilities obtained after the 15 minute treatment followed by the 42 hour post-exposure period were compared to the mean of the negative control treated tissue (See table 1 for results).
Any other information on results incl. tables
Pre-test results: The MTT solution containing trisodium 6,6',6"-(1,3,5-triazine-2,4,6-triyltriimino)trihexanoate did not turn blue/purple indicating that the test material did not directly reduce MTT.
Table 1. Mean Optical density values and % viabilities for the negative control material, positive control material and trisodium 6,6',6"-(1,3,5-triazine-2,4,6-triyltriimino)trihexanoate.
Material | OD540 of tissues | Mean OD540 of triplicate tissues | +/- SD of OD540 | Relative individual tissue viability | Relative mean % viability | +/- SD of % viability |
Negative control material | 0.745 | 0.824 | 0.071 | 90.4 | 100 | 8.5 |
0.847 | 102.8 | |||||
0.881 | 106.9 | |||||
Positive control material | 0.061 | 0.06 | 0.002 | 7.4 | 7.3 | 0.23 |
0.058 | 7 | |||||
0.061 | 7.4 | |||||
trisodium 6,6',6"-(1,3,5-triazine-2,4,6-triyltriimino)trihexanoate | 0.834 | 0.881 | 0.048 | 101.2 | 106.9 | 5.85 |
0.879 | 106.7 | |||||
0.93 | 112.9 |
Applicant's summary and conclusion
- Interpretation of results:
- not irritating
- Remarks:
- Migrated information Criteria used for interpretation of results: expert judgment
- Conclusions:
- The relative mean viability of Trisodium 6,6',6"-(1,3,5-triazine-2,4,6-triyltriimino)trihexanoate was 106.9% after a 15-minute exposure period and therefore is considered to be non-irritant.
- Executive summary:
In a primary dermal irritation study reconstituted human epidermis model (EPISKIN) was dermally exposed to 10 +/- 2mg of trisodium 6,6',6"-(1,3,5-triazine-2,4,6-triyltriimino)trihexanoate without a vehicle for 15 minutes. The EPISKIN was incubated for 42 hours, followed by an MTT test. Irritation was scored by the method of % cell viability.
In this study, trisodium 6,6',6"-(1,3,5-triazine-2,4,6-triyltriimino)trihexanoate is not a dermal irritant.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
![ECHA](/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/echa_logo.png)