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EC number: 256-283-8 | CAS number: 46830-22-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 11/03/1992
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Well documented report of a guideline study conducted to GLP.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 992
- Report date:
- 1992
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Deviations:
- no
- GLP compliance:
- yes
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Benzyldimethyl[2-[(1-oxoallyl)oxy]ethyl]ammonium chloride
- EC Number:
- 256-283-8
- EC Name:
- Benzyldimethyl[2-[(1-oxoallyl)oxy]ethyl]ammonium chloride
- Cas Number:
- 46830-22-2
- Molecular formula:
- C14H20NO2.Cl
- IUPAC Name:
- benzyldimethyl[2-(prop-2-enoyloxy)ethyl]azanium chloride
- Details on test material:
- - Name of test material (as cited in study report): ADAMQUAT BZ 80
- Physical state: liquid
- Analytical purity: 78.5%
- Impurities (identity and concentrations): Acrylic acid (0.54%), dimethylaminoethyacrylate (0.55%); EMHQ (0.0378%)
- Composition of test material, percentage of components: 100%
- Purity test date: 06/12/1991
- Lot/batch No.: op 611/613/615/616
- Expiration date of the lot/batch: 12/1992
- Stability under test conditions: stable
- Storage condition of test material: inthe dark at room temperature
Constituent 1
Method
- Target gene:
- Histidine operon
Species / strain
- Species / strain / cell type:
- other: S. typhimurium TA1535, TA1537, TA1538, TA98 and TA100
- Metabolic activation:
- with and without
- Metabolic activation system:
- S9-mix (from livers of male Sprague-Dawley rats treated by Aroclor 1254)
- Test concentrations with justification for top dose:
- 0, 125, 250, 500, 1000 and 2000 µg/plate
- Vehicle / solvent:
- distilled water
Controls
- Untreated negative controls:
- yes
- Remarks:
- vehicle
- Negative solvent / vehicle controls:
- yes
- Remarks:
- distilled water
- True negative controls:
- no
- Positive controls:
- yes
- Remarks:
- - without S9-mix: for TA1535 and TA100: sodium azide (1 µg/plate); for TA1537: 9-aminoacridine (50 µg/plate); for TA1538 and TA98: 2-nitrofluorene (0.5 µg/plate) - with S9-mix: for all strains: 2-aminoanthramin (2 µg/plate); for TA1535 and TA1537 (1 µg/
- Positive control substance:
- no
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: in agar (plate incorporation)
- incubation temperature : 37°C
DURATION
- Preincubation period: 1h
- Exposure duration: 48 to 72 h
- Expression time (cells in growth medium):
- Selection time (if incubation with a selection agent):
- Fixation time (start of exposure up to fixation or harvest of cells):
NUMBER OF REPLICATIONS: 3
NUMBER OF CELLS EVALUATED:
DETERMINATION OF CYTOTOXICITY
Bacterial toxicity: determined by examination of background lawn growth on TA100
- Number of revertants / plate
OTHER EXAMINATIONS:
- Determination of polyploidy:
- Determination of endoreplication:
- Other:
OTHER: - Evaluation criteria:
- Result is considered as positive when the following criteria are observed:
- dose-related increase in revertant colony count
- the number of revertant colonies per plate with the test substance is more than twice that of negative control.
- reproducibility of results - Statistics:
- No data
Results and discussion
Test results
- Species / strain:
- other: S. typhimurium TA1535, TA1537, TA1538, TA98 and TA100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- at 2500 µg/plate with S9 in TA 100
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Remarks on result:
- other: strain/cell type: TA 1538 and TA 100
- Remarks:
- Migrated from field 'Test system'.
Any other information on results incl. tables
CYTOTOXICITY: The test substance was toxic at the concentrations ≥ 2500 µg/plate for the TA 100 strain with and without S9 mix: the number of revertants decreased. At lower concentrations, no toxicity was observed.
GENOTOXICITY: No genotoxicity was observed in both experiments. The selected concentrations were 125, 250, 500, 1000 and 2000 µg/plate with and without the metabolic activation system S9 mix. The negative and solvent control results were equivalent to those usually obtained in our Laboratory. The number of revertants induced by the positive controls were higher than the controls, which demonstrated the sensitivity of the test and the effective activation of 2-anthramine by the S9 mix under the experimental conditions. The number of revertants obtained in the presence of ADAMQUAT BZ 80 with and without S9 mix for the 5 strains was equivalent to that of the negative and/or solvent controls.
Applicant's summary and conclusion
- Conclusions:
- ADAMQUAT BZ 80 did not show mutagenic activity in the Ames test.
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