Registration Dossier
Registration Dossier
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EC number: 617-590-6 | CAS number: 846023-24-3
Eye irritation
Administrative data
- Endpoint:
- eye irritation: in vitro / ex vivo
- Type of information:
- experimental study
- Adequacy of study:
- other information
- Study period:
- 18 August 2009
- Reliability:
- 1 (reliable without restriction)
Data source
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- other: The Ocular Toxicity Working Group (OTWG) of the Interagency Coordinating Committee on the Validation of Alternative Methods (ICCVAM) and the National Interagency Centre for the Evaluation of Alternative Toxicological Methods (NICEATM), Background Review D
- Qualifier:
- according to guideline
- Guideline:
- other: In Vitro Techniques in Toxicology Database (INVITTOX) protocol 124. Bovine Opacity and Permeability Assay - SOP of Microbiological Associates Ltd., 1999.
- Qualifier:
- according to guideline
- Guideline:
- other: Gautheron P., Dukic M., Alix D. and Sina J.F., Bovine corneal opacity and permeability test: An in vitro assay of acular irritancy. Fundam Appl Toxicol 18:442-449, 1992.
- Qualifier:
- according to guideline
- Guideline:
- other: Organisation for Economic Co-operation and Development (OECD), OECD Guidelines for Testing of Chemicals; Draft proposal for a new guideline: "Bovine Corneal Opacity and Permeability (BCOP) Test Method for Identifying Ocular Corrosives and Severe Irritants
- GLP compliance:
- yes
Test material
- Reference substance name:
- 2-cyano-N-(2,4-dichloro-5-methoxyphenyl)acetamide
- EC Number:
- 617-590-6
- Cas Number:
- 846023-24-3
- Molecular formula:
- C10H8Cl2N2O2
- IUPAC Name:
- 2-cyano-N-(2,4-dichloro-5-methoxyphenyl)acetamide
- Details on test material:
- Molecular formula C10H8Cl2N2O2
Molecular weight 259.09
CAS Number 846023-24-3
Description: Ivory flakes (determined at NOTOX)
Batch 18238505
Purity 97.3%
Test substance storage: At room temperature in the dark
Stability under storage conditions: Stable
Constituent 1
Test animals / tissue source
- Species:
- other: Bovine eye
Test system
- Controls:
- not required
- Duration of treatment / exposure:
- 240 minutes
Results and discussion
In vivo
Results
- Irritation parameter:
- cornea opacity score
- Basis:
- other: eye bovine
- Time point:
- other: 240
- Score:
- ca. 0
- Max. score:
- 0
Any other information on results incl. tables
The corneas were all clear after the 240 minutes of treatment with WAY-186478. Hence, the in vitro irritancy scores ranged from 0 to 1.2 after 240 minutes of treatment with WAY-186478.
Applicant's summary and conclusion
- Interpretation of results:
- not irritating
- Remarks:
- Migrated information
- Conclusions:
- Finally, it is concluded that this test is valid and that WAY-186478 is a non irritant in the Bovine Corneal Opacity and Permeability test under the experimental conditions described in this
report.
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