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EC number: 700-906-2 | CAS number: 22450-96-0
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
Description of key information
Assessment of Ready Biodegradability by Respirometry, OECD Guideline 301F
Key value for chemical safety assessment
- Biodegradation in water:
- readily biodegradable but failing 10-day window
Additional information
The ready biodegradability of the test material was determined in accordance with the OECD Guideline 301F.
Mean oxygen consumption in mixtures containing SABoTBA was equivalent to 24% of the theoretical value (25 mgO2/500 mL) after approximately 2 days, 63% after approximately 26 days and 67% at the end of the test (Day 28). This pattern of degradation indicates that the microbial population had adapted to the degradation products following the primary (hydrolytic) degradation of the test material.
The results obtained for the rate of degradation of sodium benzoate (60% of its ThOD after 2 days) and for the cumulative amount of oxygen consumed by the control mixtures (36.06 and 42.76 mgO2/L) fulfilled the validity criteria for this test. Comparing the ATU control with the blank controls, suggests that nitrification had occurred, which is implicated in causing the conversion of nitrogen naturally present in the cultures during the decline phase of the degradation curve, as cells begin to lyse and nutrient turnover processes occur when the sodium benzoate is exhausted from the culture. This may be why the % degradation at the end of the study in the reference culture was higher than expected.
In the presence of SABoTBA the degradation of sodium benzoate achieved 64% after 3 days indicating that the test substance was not inhibitory to the microbial inoculum.Substances are considered to be readily biodegradable in this type of test if oxygen consumption is equal to or greater than 60% of the ThOD of the test mixtures within ten days of the consumption achieving 10%. Therefore, SABoTBA was not considered to be readily biodegradable under the conditions of this test, although 60% pass level was achieved by the Day 28.
SABoTBA can therefore be described as readily biodegradable, but failing the 10 day window.
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