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EC number: 247-987-6 | CAS number: 26762-92-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: standard protocol, non GLP, tables available on results of the test substance
Data source
Reference
- Reference Type:
- publication
- Title:
- Unnamed
- Year:
- 1 988
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Deviations:
- no
- Principles of method if other than guideline:
- A test for the ability of the test substance to induce mutations in strains of Salmonella typhimurium was made. Concurrent solvent and positive controls were run with each trial. Histidine-independent (his+) colonies arising on these plates were counted following two days incubation.
- GLP compliance:
- no
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- p-menthane hydroperoxide
- IUPAC Name:
- p-menthane hydroperoxide
- Reference substance name:
- 1-methyl-1-(4-methylcyclohexyl)ethyl hydroperoxide
- EC Number:
- 201-281-4
- EC Name:
- 1-methyl-1-(4-methylcyclohexyl)ethyl hydroperoxide
- Cas Number:
- 80-47-7
- IUPAC Name:
- 1-methyl-1-(4-methylcyclohexyl)ethyl hydroperoxide
- Reference substance name:
- Menthane, monohydroperoxy derivative
- EC Number:
- 247-987-6
- EC Name:
- Menthane, monohydroperoxy derivative
- Cas Number:
- 26762-92-5
- Molecular formula:
- C10H20O2
- IUPAC Name:
- menthane, monohydroperoxy derivative
- Details on test material:
- Purity: label purity: 55%
Batch No.: not specified
Constituent 1
Constituent 2
Constituent 3
Method
- Target gene:
- Histidine
Species / strainopen allclose all
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Details on mammalian cell type (if applicable):
- - Type and identity of culture media: Oxoid No.2 broth
- Properly maintained: yes
No additional data
- Species / strain / cell type:
- S. typhimurium TA 97
- Details on mammalian cell type (if applicable):
- - Type and identity of culture media: Oxoid No.2 broth
- Properly maintained: yes
No additional data
- Metabolic activation:
- with and without
- Metabolic activation system:
- rat and hamster S-9 mix
- Test concentrations with justification for top dose:
- TA100 without S9: 0, 1, 3, 10, 33, 100 μg/plate
TA100 with S9: 0, 3, 10, 33, 100, 333 μg/plate (10% HLI); 0, 10 33, 100, 333, 666 μg/plate (10% RLI)
TA 1535 without S9: 0, 0.3, 1, 3, 10, 16 μg/plate
TA 1535 with S9: 0, 3, 10, 33, 100, 333 μg/plate (10% HLI); 0, 10, 33, 100, 333, 666 μg/plate (10% RLI)
TA 1537 without S9: 0, 1, 3, 10, 33, 66 μg/plate
TA 1537 with S9: 0, 10, 33, 100, 166, 333 μg/plate (5, 10 and 30% RLI)
TA 97 without S9: 0, 1, 3, 10, 33, 66 μg/plate
TA 97 with S9: 0, 3, 10, 33, 100, 333 μg/plate (10% HLI); 0, 10, 33, 100, 166, 333 μg/plate (5, 10 and 30% RLI)
TA 98 without S9: 0, 1, 3, 10, 33, 100 μg/plate
TA 98 with S9: 0, 3, 10 33, 100, 333 μg/plate (10% HLI); 0, 10, 33, 100, 333, 666 μg/plate (10% RLI) - Vehicle / solvent:
- - Vehicle(s)/solvent(s) used: DMSO
No additional data.
Controlsopen allclose all
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- sodium azide
- Remarks:
- In the absence of S9 mix for strains TA1535 and TA100
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- 9-aminoacridine
- Remarks:
- In the absence of S9 mix for strains TA97 and TA 1537
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- other: 4-nitro-o-phenylenediamine
- Remarks:
- In the absence of S9 mix for strains TA98
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- other: 2-aminoanthracene
- Remarks:
- In the presence of S9 mix for all strains
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: preincubation
DURATION
- Preincubation period: The test substance (0.05 mL), Salmonella culture (0.10 mL) and S-9 mix or buffer (0.50 mL) were incubated at 37 ℃, without shaking, for 20 min.
- Exposure duration: 2 days
NUMBER OF REPLICATIONS: 1 without S9; 1-3 with different concentration of S9
No additional data. - Evaluation criteria:
- A chemical was judged mutagenic (+) or weakly mutagenic (+W) if it produced a reproducible dose-related response over the solvent control in replicate trails. A chemical was judged questionable (?) if the result of individual trials were not reproducible, if increases in his+ revertants did not meet the criteria for a “+W” response, or if only single dose produced increases in his+ revertants in repeat trials. Chemicals were judged nonmutagenic (-) if they did not meet the criteria for a mutagenic of questionably response.
- Statistics:
- None stated.
Results and discussion
Test resultsopen allclose all
- Key result
- Species / strain:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- not specified
- Vehicle controls validity:
- valid
- Positive controls validity:
- valid
- Key result
- Species / strain:
- S. typhimurium TA 97
- Metabolic activation:
- with and without
- Genotoxicity:
- ambiguous
- Remarks:
- positive with metabolic activation, negative without metabolic activation
- Cytotoxicity / choice of top concentrations:
- not specified
- Vehicle controls validity:
- valid
- Positive controls validity:
- valid
- Additional information on results:
- RANGE-FINDING/SCREENING STUDIES:
The toxicity assay was performed using TA100 indicating a slight reduction of bacterial background lawn at 100 and 666 μg/plate (without and with S9 resp.)
Any other information on results incl. tables
No dose dependent increase in the number of His+ revertants was observed in TA100, TA1535, TA1537 and TA98 with or without metabolic activation
No dose dependent increase in the number of His+ revertants was observed in TA97 without metabolic activation
A dose dependent increase in the number of His+ revertants was observed in TA97 with metabolic activation (rat S9 only), not reaching a two-fold increase compared to DMSO controls at the highest tested concentration
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results
negative without metabolic activation
ambiguous with metabolic activation
The test substance did not induce mutations in Salmonella typhimurium strains without metabolic activation
With metabolic activation the results of the Salmonella typhimurium assay with test substance were ambigeous. - Executive summary:
The test substance was tested for its ability to induce mutations in strains of Salmonella typhimurium. Concentrations ranged from 0 -333 μg/plate (cytotoxicity at 666 μg/plate)
Concurrent solvent and positive controls were run with each trial. The histidine-independent (his+) colonies arising on these plates were counted following two days incubation.The test substance did not induce mutations in Salmonella typhimurium strains without metabolic activation
With metabolic activation the results of the Salmonella typhimurium assay with test substance were ambigeous.
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