Alcohols, C8-14, γ-ω-perfluoro

Endpoint summary

Additional information

Short-term toxicity to fish

A static limit test (FAZ84111) was conducted with a saturated solution. The test vessel was coated with the test item for 14 days before the test started. Duration of the test was 96 hours. Thirty test organisms were exposed to the saturated solution and the control. For the preparation of the saturated solution a suspension with a nominal loading of 100 mg/L was shaken for 96 hours and centrifuged. The clear supernatant was used for the test.

All effect levels are based on the initially measured concentration of the test item (0.18 mg/L). At the end of the test the concentration of the test item was 0.0063 mg/L. Sorption to the glass of the test vessels is unlikely as the walls were already pre-coated for 14 days before the test started.

The 96-hour NOEC and LC0 was 0.18 mg/L. The 96-hour LC50 and LC100 was > 0.18 mg/L.

A static limit test (FAZ84112) was conducted with the water accomodated fraction. The test vessel was coated with the test item for 14 days before the test started. Duration of the test was 96 hours. Thirty test organisms were exposed to the water accomodated fraction and the control. For the preparation of the water accomodated fraction, a suspension with a nominal loading of 100 mg/L was shaken for 96 hours. After phase separation, the clear liquid phase was used for the test.

All effect levels are based on the initially measured concentration of the test item (0.14 mg/L). At the end of the test the concentration of the test item was below the detection limit of 0.009 mg/L. Sorption to the glass of the test vessels is unlikely as the walls were already pre-coated for 14 days before the test started.

The 96-hour NOEC and LC0 was 0.14 mg/L. The 96-hour LC50 and LC100 was > 0.14 mg/L.

Short-term toxicity to aquatic invertebrates

A static limit test (DAI84111) was conducted with the water accomodated fraction. The test vessel was coated with the test item for 14 days before the test started. Duration of the test was 96 hours. Thirty test organisms were exposed to the water accomodated fraction and the control. For the preparation of the water accomodated fraction, a suspension with a nominal loading of 100 mg/L was shaken for 96 hours. After phase separation, the clear liquid phase was used for the test.

All effect levels are based on the initially measured concentration of the test item (0.14 mg/L). At the end of the test the concentration of the test item was below the detection limit of 0.009 mg/L. Sorption to the glass of the test vessels is unlikely as the walls were already pre-coated for 14 days before the test started.

The 96-hour NOEC and LC0 was 0.14 mg/L. The 96-hour LC50 and LC100 was > 0.14 mg/L.

A limit test (DAi84113) with the water accomodated fraction was conducted on test item. A coating period of 14 days was carried out prior to study start. The study was conducted under static conditions for a period of 48 hours. Twenty test organisms were exposed to the water accomodated fraction and control. The water accomodated fraction was analytically verified by GC analysis. The mean

measured concentration of the water accomodated fraction was 0.70 mg/L.

At the water accomodated fraction concentration of 0.70 mg/L, no biologically significant effect was determined. No immobilization was observed in either the control or the saturated solution at 24 or 48-hours. The 48-hour NOEC and EC0 values were 0.70 mg/L. The 48-hour EC50 value was > 0.70 mg/L.

Water quality parameters of pH and dissolved oxygen concentration measured at 0 and 48 hours were determined to be within acceptable limits. The validity criteria of the test guideline were fulfilled.

Long-term toxicity to aquatic invertebrates

One study evaluating the long-term toxicity of 8:2 FTOH (CAS No. 678-39-7) to Daphnia magna is available (Noack, 2003). The limit-test was conducted according to OECD Guideline 211, under GLP conditions. After 21 days of exposure in a semi-static water regime, which was chosen based on preliminary investigations due to the high volatility of the test substance, the test resulted in a NOEC (geometric mean measured concentration) of 45.3 µg/L.

Toxicity to aquatic algae

The study (SSO84111) was conducted under static conditions over a duration of 72 hours with an initial cell density of 10⁴cells/mL and a saturated solution as limit concentration, containing 0.20 mg/L. For the preparation of the saturated solution a suspension with a nominal loading of 100 mg/L was shaken for 96 hours and centrifuged. The clear supernatant was used for the test.

The test vessels were coated with the test item for 14 days before the test started. Three replicates were tested for the limit concentration and six replicates for the control. The test item was clearly dissolved throughout the test. Microscopic evaluation of the cells at study start and end of the incubation period revealed no morphological abnormalities. Water quality parameters of pH-value, measured at 0 and 72 hours, and room temperature, measured continuously, were deemed to be within acceptable limits.

The initial measured concentration of the saturated solution was 0.20 mg/L. At the end of the test the concentration of the test item was below the detection limit of 0.009 mg/L. Sorption to the glass walls of the test vessels was unlikely as the walls were already pre-coated for 14 days prior to study start.

The 72-h EbC50 (inhibition of biomass growth) > 0.20 mg/L.

The 72-h ErC50 (rate related inhibition) > 0.20 mg/L.

The 72-h NOEC for both endpoints = 0.20 mg/L.

The 72-h LOEC for both endpoints was > 0.20 mg/L.

The study (SSO84112) was conducted under static conditions over a duration of 72 hours with an initial cell density of 10⁴cells/mL and a water accomodated fraction as limit concentration, containing 0.47 mg/L. For the preparation of the water accomodated fraction a suspension with a nominal loading of 100 mg/L was shaken for 96 hours.

The test vessels were coated with the test item for 14 days before the test started. Three replicates were tested for the limit concentration and six replicates for the control. The test item was clearly dissolved throughout the test. Microscopic evaluation of the cells at study start and end of the incubation period revealed no morphological abnormalities. Water quality parameters of pH-value, measured at 0 and 72 hours, and room temperature, measured continuously, were deemed to be within acceptable limits.

The initial measured concentration of the saturated solution was 0.47 mg/L. At the end of the test the concentration of the test item was below the detection limit of 0.009 mg/L. Sorption to the glass walls of the test vessels was unlikely as the walls were already pre-coated for 14 days prior to study start.

The 72-h EbC50 (inhibition of biomass growth) > 0.47 mg/L.

The 72-h ErC50 (rate related inhibition) > 0.47 mg/L.

The 72-h LOEC for both endpoints was > 0.47 mg/L.

The 72-h NOEC for both endpoints = 0.47 mg/L.

Toxixity to microorganisms

A Respiration lnhibition Test with activated sludge according to OECD Guideline No. 209 and GLP principles was performed for 3 hrs with the test substance. The test system was activated sludge of the municipal treatment plant. The test method was static. Nominal concentrations of the test item were as follows:100, 180, 320, 580, and 1000 mg/L. The respiration rates of control, reference and test item were measured after a contact time of three hours, and the inhibitory effects of the test and reference concentrations were determined in comparison to the control respiration rates. The inhibition ranged from 2 to 9 %. The EC20, EC50 and EC80 values could not be calculated in the tested concentration range. In order to check the activity of the test system and the test conditions a reference test is carried out once every 7 days with copper-(II)-sulphate-pentahydrate as reference item and the reference toxicity is determined. The EC50 value for the reference item was 104 mg/L.

Validity criteria of the test guideline were fulfilled.

The test substance is not toxic in concentrations < 1000 mg/L to activated sludge of a municipal sewage treatment plant.