Methyl 3-methyl-2-butenoate

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1987

Report date:

1987

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 401 (Acute Oral Toxicity)

GLP compliance:

yes

Test type:

standard acute method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

3-Methylcrotonsäuremethylester

IUPAC Name:

3-Methylcrotonsäuremethylester

Constituent 2

Reference substance name:

Methyl 3-methyl-2-butenoate

EC Number:

213-107-4

EC Name:

Methyl 3-methyl-2-butenoate

Cas Number:

924-50-5

Molecular formula:

C6H10O2

IUPAC Name:

methyl 3-methylbut-2-enoate

Test material form:

other: clear liquid

Details on test material:

Purity: 97.3 %

Test animals

Species:

rat

Strain:

Wistar

Sex:

male/female

Details on test animals or test system and environmental conditions:

Age: males: 7 weeks, fermales: 8 weeks,Humidity: 50+/- 20 %, Light/dark: 12h/12h,Room temperatur: 20+/- 3 °C, Acclimatation: at least 5 days before experiment started, Stay without food: 16 h before the experiment started and 3-4 hours after administration, Feeding: Rat diet Altromin 1324 (Altromin GmbH, Lage/Lippe), ad libitum, Identification: KMnO4 (animals) and numbering of cages, 5 animal per cage (Makrolon cage type 4).

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

other: sesame oil

Details on oral exposure:

The test substance was disolved in sesame oil and administrated via tube into the gut. Occuring symptomes were investigated and documented. Once per week the body weight was measured. Animals which died during the experiment were dissected. The rats were euthanised with carbon dioxid at the end of the experiment, dissected and macroscopically examined.

Doses:

3150 mg/kg
5000 mg/kg
(10 ml/kg BW)

No. of animals per sex per dose:

5 animals

Control animals:

no

Details on study design:

The observation was 14 days after the administration of the substance.

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LD50

Effect level:

> 5 000 mg/kg bw

Based on:

test mat.

Mortality:

In the high dose group (male and female) one animal died after administration.

Gross pathology:

Animals who died during the experiment showed a brightened liver, blood in the intestines.

Any other information on results incl. tables

See: (attached background material)

Applicant's summary and conclusion

Interpretation of results:

not classified

Remarks:

Migrated information Criteria used for interpretation of results: EU

Conclusions:

The LD50 of the test item was determined to be > 5000 mg/kg bw (male and female).

Executive summary:

The LD50 of the test item was determined to be > 5000 mg/kg bw (male and female).