Registration Dossier
Registration Dossier
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EC number: 213-107-4 | CAS number: 924-50-5
Short-term toxicity to fish
Administrative data
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 987
- Report date:
- 1987
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Qualifier:
- according to guideline
- Guideline:
- other: EG Guideline C.1. Akute Toxizität für Fische, Richtlinie 84/449/EWG
- GLP compliance:
- yes
Test material
- Reference substance name:
- 3-Methylcrotonsäuremethylester
- IUPAC Name:
- 3-Methylcrotonsäuremethylester
- Reference substance name:
- Methyl 3-methyl-2-butenoate
- EC Number:
- 213-107-4
- EC Name:
- Methyl 3-methyl-2-butenoate
- Cas Number:
- 924-50-5
- Molecular formula:
- C6H10O2
- IUPAC Name:
- methyl 3-methylbut-2-enoate
- Test material form:
- other: clear liquid, colourless
- Details on test material:
- Purity: 97.3%, Storage: darkness at approximately 22 °C
Constituent 1
Constituent 2
Test organisms
- Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
Study design
- Test type:
- static
- Limit test:
- no
- Total exposure duration:
- 96 h
Test conditions
- Test temperature:
- 21.5-22.6 °C
- pH:
- 7.4-8.2
- Dissolved oxygen:
- 7.2-9.2 (mg/l)
- Details on test conditions:
- The acclimatisation time was 12 days before test start. Temperatur: 22 +/-1° C . The water was composited according ISO/DIN 7346/l, the animals were not fed during the whole test procedure.
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 96 h
- Dose descriptor:
- LC0
- Effect conc.:
- 250 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 350 - 500 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Duration:
- 96 h
- Dose descriptor:
- LC100
- Effect conc.:
- 500 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Reported statistics and error estimates:
- Probit analysis
Any other information on results incl. tables
- Sublethal observations / clinical signs:
At 500 mg/l the fish died prior the first examination, consequently no further investigations could be done. At the concentration 100 -350 mg/l the testing animals showed a lower activity and swimming under the water surface. Dead animals had a brighter colour.
Applicant's summary and conclusion
- Conclusions:
- Based on the results of this study the 96-hour LC50 of Brachydanio rerio was determined to be in the range of 350 - 500 mg/L.
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