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Diss Factsheets
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EC number: 221-897-7 | CAS number: 3271-76-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Endpoint summary
Administrative data
Description of key information
No adverse effects were observed in acute toxicity studies up to the highest dose level tested
Key value for chemical safety assessment
Acute toxicity: via oral route
Link to relevant study records
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1977/78
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- test procedure in accordance with national standard methods with acceptable restrictions
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 401 (Acute Oral Toxicity)
- Principles of method if other than guideline:
- BASF-Test
- GLP compliance:
- no
- Test type:
- standard acute method
- Limit test:
- yes
- Species:
- rat
- Strain:
- not specified
- Sex:
- not specified
- Details on test animals or test system and environmental conditions:
- no data
- Route of administration:
- oral: gavage
- Vehicle:
- water
- Details on oral exposure:
- 100% dispersion
- Doses:
- 10000 mg/kg bw
- No. of animals per sex per dose:
- 6
- Control animals:
- no
- Details on study design:
- - Duration of observation period following administration: 14 days
- Frequency of observations and weighing: no data
- Necropsy of survivors performed: yes - Statistics:
- NA
- Preliminary study:
- NA
- Key result
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- > 10 000 mg/kg bw
- Based on:
- test mat.
- Remarks on result:
- not determinable due to absence of adverse toxic effects
- Key result
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- > 2 000 mg/kg bw
- Based on:
- act. ingr.
- Remarks on result:
- not determinable due to absence of adverse toxic effects
- Mortality:
- no effects
- Clinical signs:
- no effects
- Body weight:
- no data
- Gross pathology:
- no effects
- Interpretation of results:
- GHS criteria not met
- Conclusions:
- The LD50 for rats was determined to be > 10000mg/kg bw test material (> 2000 mg/kg bw active ingredient) under the conditions of the test. The substance is not classifiable according to CLP criteria.
Reference
Endpoint conclusion
- Endpoint conclusion:
- no adverse effect observed
Acute toxicity: via inhalation route
Link to relevant study records
- Endpoint:
- acute toxicity: inhalation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1977/78
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- test procedure in accordance with national standard methods with acceptable restrictions
- Qualifier:
- according to guideline
- Guideline:
- other: Smyth H.F. et al.: Am. Ind. Hyg. Ass. J. 23, 95-107 (1960)
- GLP compliance:
- no
- Test type:
- other: BASF Method
- Limit test:
- yes
- Species:
- rat
- Strain:
- not specified
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- no data
- Route of administration:
- inhalation: aerosol
- Type of inhalation exposure:
- not specified
- Vehicle:
- other: unchanged (no vehicle)
- Details on inhalation exposure:
- Inhalation of a saturated atmosphere at ambient temperature (20°C). To achieve saturation, 200L air per hour was fed through an approx. 5 cm deep layer of the product.
- Analytical verification of test atmosphere concentrations:
- not specified
- Duration of exposure:
- 7 h
- Concentrations:
- saturation concentration
- No. of animals per sex per dose:
- 6
- Control animals:
- no
- Details on study design:
- - Duration of observation period following administration: 14 days
- Frequency of observations and weighing: daily observation, weekly weighing
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight, organ weights, histopathology - Statistics:
- NA
- Preliminary study:
- NA
- Sex:
- not specified
- Dose descriptor:
- LC0
- Based on:
- test mat.
- Exp. duration:
- 7 h
- Remarks on result:
- other: no effect at saturation concentration
- Mortality:
- no deaths
- Clinical signs:
- other: no effects
- Body weight:
- no data
- Gross pathology:
- no effects
- Interpretation of results:
- GHS criteria not met
- Conclusions:
- 7 hours inhalation of a with test item saturated atmosphere did not lead to any deaths in rats
Reference
Endpoint conclusion
- Endpoint conclusion:
- no adverse effect observed
Acute toxicity: via dermal route
Link to relevant study records
- Endpoint:
- acute toxicity: dermal
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1974
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study with acceptable restrictions
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 402 (Acute Dermal Toxicity)
- GLP compliance:
- no
- Test type:
- standard acute method
- Limit test:
- no
- Species:
- rat
- Strain:
- not specified
- Sex:
- not specified
- Type of coverage:
- occlusive
- Vehicle:
- CMC (carboxymethyl cellulose)
- Details on dermal exposure:
- 50% concentration
- Duration of exposure:
- 20 h
- Doses:
- 2500 mg/kg bw
- No. of animals per sex per dose:
- 6
- Control animals:
- not required
- Details on study design:
- - Duration of observation period following administration: 14 days
- Necropsy of survivors performed: yes - Key result
- Sex:
- not specified
- Dose descriptor:
- LD50
- Effect level:
- > 2 500 mg/kg bw
- Based on:
- test mat.
- Remarks on result:
- not determinable due to absence of adverse toxic effects
- Mortality:
- none
- Clinical signs:
- none
- Body weight:
- no data
- Gross pathology:
- no effects
- Interpretation of results:
- GHS criteria not met
- Conclusions:
- No adverse effects were noted at a limit dose of 2500 mg/kg bw
Reference
Endpoint conclusion
- Endpoint conclusion:
- no adverse effect observed
Additional information
The acute oral toxicity of Vat Green 3 was tested in male and female rats at a limit dose level of 10000 mg/kg bw. The animals were observed for 14 days after test substance administration. No deaths or clinical signs of toxcity were observed throughout the study.
The LD50 was determined to be higher than 10000 mg/kg bw.
The acute inhalation toxicity of Vat Green 3 was tested in rats in a saturated atmosphere for 7 hours. The animals were observed for 14 days after test substance administration. No deaths or clinical signs of toxcity were observed throughout the study.
The acute dermal toxicity of Vat Green 3 was tested in male and female rats at a limit dose level of 2500 mg/kg bw. The animals were observed for 14 days after test substance administration. No deaths or clinical signs of toxcity were observed throughout the study.
The dermal LD50 was determined to be higher than 2500 mg/kg bw.
Justification for classification or non-classification
No adverse effects were observed in acute toxicity studies.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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