Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 202-617-2 | CAS number: 97-90-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Remarks:
- OECD 201, GLP, analytical monitoring.
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Version / remarks:
- (1984)
- Deviations:
- no
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- EPA OPPTS 850.5400 (Algal Toxicity, Tiers I and II) (January 2012)
- Version / remarks:
- (1996)
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- other: JMAFF No. 700, 1039 and 1014 (1986)
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- - Name of test material (as cited in study report): Ethylene Glycol Dimethacrylate
- Analytical monitoring:
- yes
- Test organisms (species):
- Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- TEST ORGANISM
- Common name: Pseudokirchneriella subcapitata
- Strain: CCAP 278/4
- Source: cultured at CIT, origin: Culture Collection of Algae and Protozoa, Institute of Freshwater Ecology, Far Sawrey, Ambleside, Cumbria,
LA22 OLP, UK
- Age of inoculum (at test initiation):
- Method of cultivation: the algae were cultured under sterile conditions and maintained at exponential growth rate by adding weekly aliquots of
algal culture to fresh reconstituted water samples.
ACCLIMATION
- Acclimation period: no data, alga were cultured at CIT
- Any deformed or abnormal cells observed: no data - Test type:
- static
- Limit test:
- no
- Total exposure duration:
- 96 h
- Remarks on exposure duration:
- closed bottle
- Hardness:
- 34 mg/L as CaCO3
- Test temperature:
- 20.5 to 22.6 °C
- pH:
- 7.79 to 10.87 (7.79 to 10.28 for the control)
- Nominal and measured concentrations:
- Nominal concentrations: 0, 0.001, 0.01, 0.1, 1.00, 10.00 and 100 mg/L corresponding to
Measured concentrations: The limit of detection (1 mg/L) was not sufficient to determine the concentrations of Ethylene Glycol Dimethacrylate in the test solutions at 0.001, 0.01 and 0.1 mg/L . The other measured concentrations in the test solutions were 0.909, 8.62 and 98.0 mg/L (the concentrations were within ± 20% of the corresponding nominal value) - Details on test conditions:
- TEST SYSTEM
- Test vessel: glass test vessels (250 mL Erlenmeyer flasks)
- Type: closed system
- Aeration: was not used during the test but the cultures were constantly agitated
- Initial cells density: The number of cells in solutions was calculated for each replicate at time 0 as 1 x 10E+4 cells/mL.
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 6
GROWTH MEDIUM
- Standard medium used: yes
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: LC reconstituted water
- Hardness: 34 mg/L ± 17 mg/L as CaCO3
- pH: 7.5 ± 0.3
OTHER TEST CONDITIONS
- Sterile test conditions: yes
- Adjustment of pH: no adjustment of pH before addition of the algae
- Photoperiod: illumination was continuous
- Light intensity and quality: 6950 lux to 7660 lux
EFFECT PARAMETERS MEASURED:
- Determination of cell concentrations: with a Malassez cell counter
TEST CONCENTRATIONS
- Limit test
- Test concentrations: 0, 100 mg/L
- Range finding study
- Test concentrations: 0.01, 0.1, 1.0 and 10 mg/L - Reference substance (positive control):
- no
- Duration:
- 96 h
- Dose descriptor:
- EC50
- Effect conc.:
- 19 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: static, closed bottle test
- Duration:
- 96 h
- Dose descriptor:
- EC50
- Effect conc.:
- 10.1 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Remarks on result:
- other: static, closed bottle test
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.804 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks:
- (< LOQ) based on both the growth rate and biomass
- Remarks on result:
- other: static, closed bottle test
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 17.3 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: static, closed bottle test
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 9.1
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Remarks on result:
- other: static, closed bottle test.
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 6.93 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: static, close bottle test
- Reported statistics and error estimates:
- The EC50 values were calculated, using geometric mean measured concentrations, according to Probit analysis The confidence interval limits were not presented because the ratio between the tested concentrations was too high to determine these limits with enough accuracy.
- Validity criteria fulfilled:
- yes
- Conclusions:
- Under the experimental conditions of this study, the 72-hour ErC50 and the 72-hour EbC50 of Ethylene Glycol Dimethacrylate (EGDMA; CAS: 97-90-5 were 17.3 and 9.10 mg/L, respectively, and the 96-hour ErC50 and the 96-hour EbC50 were 19.0 and 10.1 mg/L, respectively, in a static test
system for Pseudokirchneriella subcapitata according to guideline OECD 201.
The NOEC's were 0.906 mg/L (< LOQ) at 72 hours and 0.804 mg/L (< LOQ) at 96 hours based on both the specific growth rate data and biomass
data. - Executive summary:
In a 96 hour acute toxicity study, the cultures of Pseudokirchneriella subcapitata strain no. CCAP 278/4 were exposed to Ethylene Glycol Dimethacrylate at nominal concentrations of 0, 0.001, 0.01, 0.1, 1.0, 10.0, and 100 mg/L under static conditions in accordance with the guideline OECD 201. The validated limit of quantification of the analytical method (1 mg/L) was not sufficient to determine the concentrations of Ethylene Glycol Dimethacrylate in the nominal test solutions at 0.001, 0.01 and 0.1 mg/L. Therefore, analysis was only performed in the test solutions at 1, 10 and 100 mg/L. The control sample of time 0 confirmed that the test item concentration was below the limit of detection.
The ErC50value based on growth rate was 19.0 mg/L and
EbC50value based on biomass was 10.1 mg/L after 96 hours, respectively.
This toxicity study is classified as acceptable and satisfies the guideline requirements for this acute algal toxicity study with Pseudokirchneriella subcapitata.
Results Synopsis
Test Organism: Pseudokirchneriella subcapitata
Test Type: Static, closed bottle test
96-hr ErC50: 19.0 mg a.i./L
96-hr EbC50: 10.1 mg a.i./L
96-hr NOEC: 0.804 mg a.i./L (<LOQ)
Endpoint(s) Effected: based on growth rate and biomass
Reference
pH
values:
Concentration (mg/l) pH
Start pH
End
0 7.79 10.28
0.001 8.05 10.70
0.01 8.04 10.87
0.1 8.05 10.84
1.0 8.04 10.78
10 8.02 10.13
100 8.01 8.68
Definitive (EC50) test 96 -hour % inhibition
Nominal concentration [mg/L] |
Measured concentration [mg/L] |
Percentage inhibition growth rate | Percentage inhibition bio mass |
0 | 0 | ||
0.001 | below the limit of detection (1.0 mg/L) or below the LOQ | ||
0.01 | below the limit of detection (1.0 mg/L) or below the LOQ | ||
0.1 | below the limit of detection (1.0 mg/L) or below the LOQ | ||
1 | 0.804 (< LOQ) | 1 | 5 |
10 | 7.34 | 3 | 28 |
100 | 76.2 | 100 | 100 |
Variation coefficient of the control cell density at 96 -hours: 10.19%
At
72 hours:
ErC 10 = 6.93
mg/l
EbC 10 = 2.65 mg/l
ErC 50 = 17.3 mg/l (95%
Confidence Limits)
EbC 50 = 9.10 mg/l (95% Confidence Limits)
NOEC = 0.906 mg/l (< LOQ) based on both the growth rate and biomass
At 96-hours:
ErC 10 = 8.10 mg/l
EbC 10 = 2.30 mg/l
ErC 50 = 19.0 mg/l (95% Confidence Limits)
EbC 50 = 10.1 mg/l (95% Confidence Limits)
NOEC = 0.804 mg/l (< LOQ) based on both the growth rate and biomass
Description of key information
The toxicity of the substance to the freshwater alga Pseudokirchneriella subcapitata has been evalutaed in a 72h/96-h exposure growth inhibition test according to OECD guideline 201 (Klimisch score 1). The 72-h EC50 was found to be 17.3 mg/l and the 72-h EC10 6.93 mg/l, based on growth rate.
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 17.3 mg/L
- EC10 or NOEC for freshwater algae:
- 6.93 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.