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EC number: 274-386-6 | CAS number: 70209-87-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 29 Jul 2014 - 04 Sep 2014
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Deviations:
- no
- Principles of method if other than guideline:
- None
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- None
- Analytical monitoring:
- yes
- Details on sampling:
- After sampling, the test medium samples (0.5 mL) were mixed with 0.5 mL methanol and stored deep-frozen (below -18 °C) until analysis. At the analytical laboratory, the samples were thawed and shaken well using a Vortex mixer. If necessary, the samples were further diluted with methanol/water (1:1, v/v) prior to analysis by HPLC-PDA.
- Vehicle:
- no
- Details on test solutions:
- Each day the necessary amount of test item for preparing the stock solution was weighed on a weighing scoop and transferred to a volumetric flask. Test medium was added up to the bench mark and the solution was homogenised by shaking. The lower test solutions were prepared by dilution of the stock solution. Each solution was applied to the respective aquaria.
- Test organisms (species):
- Oncorhynchus mykiss (previous name: Salmo gairdneri)
- Details on test organisms:
- Species: Oncorhynchus mykiss (Walbaum)
Size: 4 - 6 cm
Producer: Fischzucht Störk, D-88343 Wagenhausen, Germany
Acclimatisation:
Fish for the test were obtained from the supplier on 15 July 2014 (acclimatisation > 12 days) and held under testing conditions in 300 L containers with continuous renewal of water (5 - 10 % per day) and permanent aeration of water. The fish were held under identical conditions for the entire pre-experimental period. The last feeding was performed 24 hours prior test start.
Holding water:
Water composed of dechlorinated drinking water and deionised water. The pH-value of the aerated water was between 6.0 - 8.5. The dissolved oxygen was above 60 % saturation and the total hardness was between 140 - 250 mg/L (as CaCO3), corresponding to 7.8 - 14°dH.
Light: 12 to 16 hours daily.
Temperature: The water temperature was 15 to 17 °C.
Oxygen: The concentration of oxygen was controlled 3 times a week. The air saturation value was ≥ 60 %.
Feeding: The fish were fed each day with granular rearing food with approx. 2 % of their body-weight. The food was obtained from the fish supplier.
Mortality: Following a 48-hour settling-in period, mortality was recorded. No mortality above 5 % was observed throughout the acclimatisation period. - Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Hardness:
- 8°dH corresponding to 143 mg CaCO3/L
- Test temperature:
- 15.2 – 16.9 °C
- pH:
- 7.69 – 8.47
- Dissolved oxygen:
- ≥ 97 % of air saturation
- Nominal and measured concentrations:
- see table below
- Details on test conditions:
- Test procedure: semi-static test
Duration: 96 hours
Temperature: 15.2 – 16.9 °C
Oxygen concentration: ≥ 97 % of air saturation
pH-value: 7.69 – 8.47
Hardness: 8°dH corresponding to 143 mg CaCO3/L
Exposure to light: 16 hours daily
Feeding: none
Test vessels: 30 L glass aquaria, filled with 15 L
Loading: max. loading of 1 g fish/L
Aeration: continuous aeration of the test tanks with a membrane pump using a Pasteur pipette
Number of animals: 7 per concentration, no replicates - Reference substance (positive control):
- no
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 3.7 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: 95% CL: 1.92-5.54 mg/L
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 2.07 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Details on results:
- Mortality:
In the control and at the test item concentrations up to and including 0.939 mg/L no mortality was observed within the period of the test. At 2.07 mg/L one fish died by accident after 72 hours and at 4.55 mg/L five fish were found dead after 96 hours. At the highest test item concentration of 10.0 mg/L all fish were found dead after 4 hours.
Observations:
No sublethal effects were observed in the control and at the test item concentration up to and including 0.939 mg/L after 96 h.
At 2.07 mg/L three fish showed reduced activity and dark pigmentation after 24 hours. After 72 hours one fish died by accident.
At 4.55 mg/L six fish showed reduced activity after 24 hours and one fish was dead. After 48 hours five fish showed reduced activity and three of them showed dark pigmentation. Two fish were dead. After 72 hours three fish showed reduced activity and four fish were dead. After 96 hours one fish showed reduced activity and one fish had lost equilibrium. Five fish were dead.
At 10.0 mg/L all fish were found dead after four hours.
All fish were weighed and measured. The average weight of the test organisms was 0.88 +/- 0.25 g; the average length was 46 +/- 3 mm. - Reported statistics and error estimates:
- The NOEC (mortality) was established based on the highest test item concentration at which the mortality is not higher than the allowed control mortality.
The LC50-values after 4, 24, 48, 72 and 96 h were calculated using Weibull analysis.For evaluation, the statistical programme ToxRat Professional 2.10 was used. - Sublethal observations / clinical signs:
LC50-values of fish exposed to the test item
FAT 20060/F TE nominal [mg/L]
Time
[h]NOEC
mortality
[mg/L]LC501)
[mg/L]95 % confidence limit of LC50
[mg/L]4
4.55
8.83
-
24
4.55
5.99
-
48
2.07
5.42
3.65 – 244
72
2.07
4.13
2.45 – 7.23
96
2.07
3.70
1.92 – 5.54
1) calculated using Weibull analysis
- not calculable
- Validity criteria fulfilled:
- yes
- Conclusions:
- The LC50 (96 h) of the test item was determined to be 3.70 mg test item/L (nominal).
- Executive summary:
FAT 20060/F TE was evaluated for short term toxicity to fish according to OECD Guideline 203.
The content of FAT 20060/F TE in test solutions was analytically determined to confirm the correct application of the test item. The mean measured content of FAT 20060/F TE in fresh test solutions was between 84 % and 104 % of nominal. The measured content of FAT 20060/F TE in aged test solutions was between 86 % and 99 % of nominal. Since the measured concentrations of FAT 20060/F TE in aged test item solutions were between 80 and 120 % of nominal concentrations, the biological endpoints were evaluated using nominal test item concentration.
The total hardness (as CaCO3) of the test water was determined to be 8°dH; the mean pH-value of the control was determined to be 8.24 +/- 0.20. The mean temperature of the untreated control and all test item concentrations was measured to be 16.1 +/- 0.5 °C and the oxygen saturation was determined to be 99 +/- 1 %.
LC50-values of fish exposed to the test item
FAT 20060/F TE nominal [mg/L]
Time
[h]NOEC
mortality
[mg/L]LC501)
[mg/L]95 % confidence limit of LC50
[mg/L]4
4.55
8.83
-
24
4.55
5.99
-
48
2.07
5.42
3.65 – 244
72
2.07
4.13
2.45 – 7.23
96
2.07
3.70
1.92 – 5.54
1) calculated using Weibull analysis
- not calculable
Reference
Description of key information
The 96 h LC50 of Acid Red 315 was considered to be 3.7 mg/L.
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 3.7 mg/L
Additional information
Short term toxicity of Acid Red 315 to fish was evaluated in the following studies:
In a study conducted according to OECD Guideline 203, the LC50 (96 h) of FAT 20060/F was determined to be 3.70 mg test item/L (nominal). The corresponding NOEC (mortality) (96 h) was 2.07 mg test item/L (nominal). No sublethal effects were observed up to and including 0.939 mg test item/L (nominal) after 96 h. FAT 20060/B was also evaluated for short-term toxicity to fish. In this study, the 48 h LC0 was 2 mg/L (i.e. 5.2 mg a.i./L), the 48 h LC50 was 6.3 mg/L and the 48 h LC100 was 20 mg/L (i.e. 16.4 mg a.i./L).
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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