N-cyclopropyl-1,3,5-triazine-2,4,6-triamine

Toxicity to birds

Administrative data

Endpoint:

short-term toxicity to birds

Type of information:

experimental study

Adequacy of study:

supporting study

Study period:

1978/08/11 to 1978/08/19

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1978

Report date:

1978

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 205 (Avian Dietary Toxicity Test)

Deviations:

yes

Remarks:

No analytical verification of test item in diet

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

N-cyclopropyl-1,3,5-triazine-2,4,6-triamine

EC Number:

266-257-8

EC Name:

N-cyclopropyl-1,3,5-triazine-2,4,6-triamine

Cas Number:

66215-27-8

Molecular formula:

C6H10N6

IUPAC Name:

N2-cyclopropyl-1,3,5-triazine-2,4,6-triamine

Dose method:

homogenously mixed into feed (accounts for technical substances)

Analytical monitoring:

no

Vehicle:

yes

Remarks:

olive oil

Details on preparation and analysis of diet:

Homogeneous substance feed mixtures were prepared by blending a 1 and 10 % dilution of cyromazine or Dieldrin in olive oil (oleum olivae PH H VI) into pulverized commercial food.

Test organisms

Test organisms (species):

other: Peking duck (anas domestica)

Details on test organisms:

Experiments were performed on Peking ducks (Anas domestica) bred and raised on the premises, with an age of 5 to 10 days.

Study design

Limit test:

no

Total exposure duration (if not single dose):

8 d

Post exposure observation period:

3 days

No. of animals per sex per dose and/or stage:

Ten birds in 5 groups were established for each treatment and control

Control animals:

yes, concurrent no treatment

Nominal and measured doses / concentrations:

Nominal: 100, 600, 1000, 6000 and 10,000 mg/kg diet for cyromazine

Details on test conditions:

The birds were housed in a wure battery 10 to a cage. They were kept at a room temperature of 22 ± 1°C and a relative humidity of 55 ± 5 % and had free access to feed and water.

Examinations

Details on examinations and observations:

Throughout the 8-day treatment and observation period the birds were observed daily for rate of deaths, changes in general conditions and food consumption. Food consumption though measured accurately, was presented as approximate consumption only due to the unavoidable wastage by the birds. Bodyweights were recorded at initiation, after 5 days and at the end of the study.

Reference substance (positive control):

yes

Remarks:

positive control groups treated with 5 different dieldrin concentrations: 60, 100, 200, 300 and 600 mg/kg diet (nominal)

Results and discussion

Effect levels

Effect levels 1

Key result

Duration (if not single dose):

8 d

Dose descriptor:

LC50

Effect level:

> 10 000 mg/kg diet

Conc. / dose based on:

act. ingr.

Basis for effect:

mortality

Remarks on result:

other: both male and female

Effect levels 2

Duration (if not single dose):

8 d

Dose descriptor:

LC50

Remarks:

for positive control

Effect level:

272 other: ppm

Conc. / dose based on:

other: positive control

Basis for effect:

mortality

Mortality and sub-lethal effects:

No mortalities were observed in any of the cyromazine treatments. However at the 6000 and 10,000 mg/kg diet treatments slight signs of ataxy, curved position and lethargy were noted within 2-3 days of test start although the birds had recovered by the end of the 3-day observation phase.

In concentrations with positive control, where mortalities occurred dyspnoea, jerkiness in gait, ataxy terminal wing beat convulsions and curved or ventral position were seen starting with the 2nd experimental day. These symptoms became more accentuated as the concentration was increased.

Results with reference substance (positive control):

In the positive control item, during the 5-day treatment period, there was a concentration related decrease in food consumption in groups of 200 up to 600 ppm. It was again comparable to that of the controls at the end of the 3 observation days. The bodyweight gain of the birds in concentrations of 200 up to 600 ppm was retarded during the test and observation period.

Further details on results:

Effects of food consumption:

In cyromazine concentrations from 1000 up to 10000 ppm food consumption was decreased during the 5 treatment days. There was a clear tendency of recovery at the end of the 3 observation days.



For bodyweight, bodyweights were decreased during the 5 treatment days in concentrations of 6000 and 10000 ppm. There was a clear tendency to regress during the 3 observation days.

Reported statistics and error estimates:

For birds treated with the test item an exact determination of LC50 was not possible, as in the higher concentrations the birds partially refused food during the 5 day treatment period; also no mortalities were observed at any treatment. For the positive control (dieldrin) treatment, the LC50 including 95% confidence limits were calculated by the logit model.

Applicant's summary and conclusion

Validity criteria fulfilled:

not applicable

Conclusions:

The 8-day, short term dietary LC50 of cyromazine in the Peking duck (Anas domestica) is > 10000 mg/kg diet (the highest concentration tested), when fed to "5—day old Peking ducks (Anas domestica)" of both sexes. In the higher concentrations the birds partially refused food during the 5 day treatment period, therefore an exact LC50 determination was not possible.

The 8-day LC50 for positive control (Dieldrin) is 272 (182—433) ppm when fed under the same experimental conditions.

Executive summary:

The object of the present study was to provide information on the toxic hazard of this compound to wild birds. The 8-day- toxicity of the compound was investigated in the Peking duck (Anas domestica). The procedure adopted was based on the recommendations of the United States Department of the Interior Fish and Wildlife Service, reported in "Procedure for Evaluation of Acute Toxicity of Pesticides to Fish and Wildlife" of December 14, 1964. This study did not follow any guidelines, and therefore no validity criteria could be assessed. 

 

Five groups of birds received feed with five different concentrations of the test compound for a 5-day period, the concentrations were 100, 600, 1000, 6000 and 10,000 mg/kg diet for cyromazine, and 60, 100, 200, 300 and 600 mg/kg for positive control item (dieldrin). 

 

The short term dietary LC₅₀ of cyromazine in the Peking duck (Anas domestica) is > 10000 mg/kg diet (the highest concentration tested), when fed to 5-day old Peking ducks (Anas domestica) of both sexes as no mortlaity was observed at any treatment. However, in the higher concentrations the birds partially refused food during the 5 day treatment period. The 8-day LC₅₀ for Dieldrin is 272 (182—433) ppm when fed under the same experimental conditions