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EC number: 266-257-8 | CAS number: 66215-27-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1980/11/03 to 1980/11/07
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Remarks:
- No analytical monitoring and only two organism per concentration.
- Justification for type of information:
- Study performed under GLP conditions. No deviations occurred from these guidelines.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 981
- Report date:
- 1981
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: Committee on Methods for Toxicity Tests with Aquatic Organisms. 1975. Methods for Acute Toxicity Tests with Fish, Macroinvertebrates and Amphibians. EPA-660/3-75-009. 61 pp.
- Deviations:
- yes
- Remarks:
- No replicate concentrations were used.
- GLP compliance:
- no
Test material
- Reference substance name:
- N-cyclopropyl-1,3,5-triazine-2,4,6-triamine
- EC Number:
- 266-257-8
- EC Name:
- N-cyclopropyl-1,3,5-triazine-2,4,6-triamine
- Cas Number:
- 66215-27-8
- Molecular formula:
- C6H10N6
- IUPAC Name:
- N2-cyclopropyl-1,3,5-triazine-2,4,6-triamine
Constituent 1
- Specific details on test material used for the study:
- The test material is cyromazine.
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Vehicle:
- no
- Details on test solutions:
- Dilution water for the test was obtained from a well, treated with a Continental Model 3020 Reverse Osmosis Water System, and deionized; then, following the procedures of Marking and Dawson (1973) it was reconstituted to soft water with total hardness of 44 mg/L as CaCO3, total alkalinity of 30 mg/L as CaCO3 and conductivity of 150 micromhos per centimeter. A fresh working stock solution of the test material in deionized water was prepared by weight to a precision of 1.0 mg/L.
Five concentrations were tested, with a control. Testing was begun by adding measured volumes of stock solution to the water in five test vessels, and mixing thoroughly, to yield analyzed test material concentrations of 11.8, 17.1, 28.6, 50.8 and 87.9 mg/L.
Test organisms
- Test organisms (species):
- Oncorhynchus mykiss (previous name: Salmo gairdneri)
- Details on test organisms:
- - Length at study initiation: 40 (36 - 43) mm
- Weight at study initiation: 0.49 (0.37 - 0.63) grams
- Age: approx. 2 months old
Study design
- Test type:
- static
- Water media type:
- other: Soft water
- Limit test:
- no
- Total exposure duration:
- 96 h
Test conditions
- Hardness:
- 44 mg/L
- Test temperature:
- 11.6 - 12.5 °C
- pH:
- Control water pH: 7.60
- Conductivity:
- 140 micromhos/cm
- Nominal and measured concentrations:
- Nominal concentrations : 11.8, 17.1, 28.6, 50.8 and 87.9 mg/L.
- Details on test conditions:
- The definitive test was conducted in 19.6 liter, chemically clean glass jars, each containing 15 liters of soft reconstituted dilution water and immersed in a constant temperature water bath. Two days before starting the test the fish (Oncorhynchus mykiss) were selected at random from the stock culture, taken off feed, and isolated in a jar of dilution water for 48 hours acclimation before testing. Two test organisms were introduced into each container, mortalities were recorded every 24 hours for 96 hours.
Results and discussion
Effect concentrations
- Key result
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 50.8 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Details on results:
- The 96 hour LC50 with 95 percent confidence limits of cyromazine to the rainbow trout (Oncorhynchus mykiss) could not be determined due to the lack of mortalities in all concentrations. The no effect concentration during the test period was observed to be 50.8 mg/L. In the 'Attached Background material' section, Chemical and physical parameters monitored during the test are presented in and Percent mortalities are presented. It should be noted that LC50 values may vary with different species, temperatures and water qualities.
Any other information on results incl. tables
- Sublethal observations / clinical signs:
Behavioral observations (general behavior: norreal, irritated, sounding, surfacing and respiration behaviour: mucus shedding, irregular) were made in the highest concentration of 87.9 mg/L.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The 96 hour NOEC is 50.8 mg/L.
- Executive summary:
This study was conducted to determine the static acute toxicity of cyromazine to the rainbow trout (Oncorhynchus mykiss). Test practices followed were those recommended by the Committee on Hethods for Toxicity Tests with Aquatic Organisms (1975), except that replicate concentrations were not used.
Testing was performed by directly adding the test material to the water in five test vessels, and mixing thoroughly, to yield analyzed test material concentrations of 11.8, 17.1, 28.6, 50.8 and 87.9 mg/L. Ten Oncorhynchus mykiss were introduced at random into each of the test and control vessels. These were approximately 2 months old with a mean length of 40 mm and mean weight of 0.33 grams.
The 96 hour LC50 of cyromazine to the rainbow trout (Oncorhynchus mykiss) could not be determined due to the lack of mortalities in all concentrations. The no effect concentration (NOEC) during the test period was observed to be 50.8 mg/L.
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