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EC number: 406-940-1 | CAS number: 126019-82-7 DP 211
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP guideline study with analytical monitoring
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 002
- Report date:
- 2002
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Version / remarks:
- (91/69/EEC C.3)
- GLP compliance:
- yes
Test material
- Reference substance name:
- O,O,O-tris(2(or 4)-C9-10-isoalkylphenyl) phosphorothioate
- EC Number:
- 406-940-1
- EC Name:
- O,O,O-tris(2(or 4)-C9-10-isoalkylphenyl) phosphorothioate
- Cas Number:
- 126019-82-7
- Molecular formula:
- C45 H69 O3 P S
- IUPAC Name:
- O-2-(2-methylnonyl)phenyl O-4-(2-methylnonyl)phenyl O-2-(2-methyloctyl)phenyl phosphorothioate
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- For the analysis of the actual test item concentrations the following samples were taken:
Just before the start of the test:
- duplicate samples from each test medium (without algae)
- duplicate samples from the control (without algae)
After 72 hours:
- duplicate samples from each test medium (without algae)
- duplicate samples from the control (without algae)
For the 72-hour stability samples additional flasks with adequate volumes of the freshly prepared test media of all test concentrations and the control were incubated under the same conditions as in the actual test but without algae. All samples were analyzed immediately after sampling. The concentrations of the test item were analyzed in all test medium samples from both sampling times (0 and 72 hours). From the control samples only
one of the duplicate samples was analyzed from each of both sampling times.
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Five WAFs (Water Accommodated Fractions) were prepared. Four days prior to the start of the test, five individual mixtures with loading rates of 4.6, 10, 22, 46 and 100 mg/L were prepared by weighing 11.9, 10.7, 15.5, 32.9, and 70 mg of test item into 2.5, 1.0, 0.70, 0.70, and 0.70 liter test water, respectively. No auxiliary solvent or emulsifier was used. The test item was mixed into the test water as homogeneously as possible by an ultrasonic treatment for 15 minutes and intense stirring for 96 hours on a magnetic stirrer at room temperature in the dark. The long stirring period of 96 hours was chosen to dissolve a maximum concentration of the test item in test water. The results of a pre-test (without GLP) have shown that during this time no significant loss of the test item was to be expected. These saturated aqueous dispersions were filtered through a membrane filter (Schleicher & Schuell, Type NC20, pore size 0.2 µm) just before the start of the test. With reduced negative pressure the dispersions were filtered as carefully as possible to avoid, that the liquid test item was sucked through the filter. The filtrates of the dispersions with dissolved test item were tested on algae as WAFs. Additionally, a control was tested in parallel.
Test organisms
- Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- TEST ORGANISM
- Common name: green algae
- Strain: No. 86.81 SAG
- Source: Sammlung von Algenkulturen Göttingen (SAG, Experimentelle Phykologie und Sammlung von Algenkulturen, Albrecht-von-Haller-lnstitut für Pflanzenwissenschaften, Universität Göttingen, D-37073 Göttingen, Germany)
- Method of cultivation: according to test guidelines
ACCLIMATION
- Acclimation period: 4 days
- Culturing media and conditions: Same conditions as test
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
Test conditions
- Hardness:
- 24 mg/L as CaCO3
- Test temperature:
- 22-23 °C
- pH:
- 7.9 - 8.1
- Nominal and measured concentrations:
- Nominal (loading rate): 4.6, 10, 22, 46, 100 mg/L
Mean measured concentrations: - Details on test conditions:
- TEST SYSTEM
- Test vessel: 50 mL Erlenmeyer flasks covered with glass dishes
- Aeration: None
- Initial cells density: 10000 cells/mL
- Control end cells density: 62.28 x 10000 cells/mL
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 6
GROWTH MEDIUM
- Standard medium used: yes
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: According to guideline
- Culture medium different from test medium: No
- Intervals of water quality measurement: Daily
OTHER TEST CONDITIONS
- Photoperiod: Continuously illumination
- Light intensity and quality: 9200 lux (mean value)
EFFECT PARAMETERS MEASURED
- Determination of cell concentrations: Electronic particle counter (Coulter Counter, Model ZM)
TEST CONCENTRATIONS
- Spacing factor for test concentrations: ca. 2.2 - Reference substance (positive control):
- no
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Basis for effect:
- growth rate
- Remarks on result:
- other: Value based on loading rate
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Basis for effect:
- biomass
- Remarks on result:
- other: Value based on loading rate
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Basis for effect:
- growth rate
- Remarks on result:
- other: Value based on loading rate
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Basis for effect:
- biomass
- Remarks on result:
- other: Value based on loading rate
- Details on results:
- - Exponential growth in the control: yes
- Any stimulation of growth found in any treatment: No
- Effect concentrations exceeding solubility of substance in test medium: Yes
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
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