Registration Dossier
Registration Dossier
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EC number: 607-088-5 | CAS number: 224785-90-4
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2000
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.4-E (Determination of the "Ready" Biodegradability - Closed Bottle Test)
- Deviations:
- no
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)
- Deviations:
- no
- GLP compliance:
- yes
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- other: mixed population of aquatic microorganisms
- Details on inoculum:
- Source: secondary effluent of a domestic sewage treatment plant (STP Odenthal, Germany)
Date of collection: 2000-01-21
Preconditioning: separation of coarse particles by filtration; aeration of mineral medium plus inoculum (filtrate) for 6 days
Effluent concentration in reaction mixture: 5 mL/L - Duration of test (contact time):
- 28 d
- Initial conc.:
- 4.1 mg/L
- Based on:
- test mat.
- Details on study design:
- Pretreatment of test substance:
The test substance was weighed out on aluminium foil. This substance inclusive the aluminium foil was added to the test vessel.
Exposure conditions:
Incubation time: 28 d
Incubation temperature: 20 - 21°C - Reference substance:
- benzoic acid, sodium salt
- Remarks:
- Purity: 99%; initial concentration: 2.9 mg/l
- Parameter:
- % degradation (O2 consumption)
- Value:
- 0
- Sampling time:
- 28 d
- Value:
- 1 703 mg O2/g test mat.
- Results with reference substance:
- 77 % degradation of benzoic acid, sodium salt within 14 days, and 81 % degradation within 28 days.
- Validity criteria fulfilled:
- yes
- Remarks:
- 77 % degradation of the reference compound benzoic acid, sodium salt within 14 days
- Interpretation of results:
- under test conditions no biodegradation observed
- Conclusions:
- With 0 % degradation within 28 days the substance is considered as not readily biodegradable.
- Executive summary:
Using the EU Method C.4-E (Determination of the "Ready" Biodegradability - Closed Bottle Test) 0 % degradation within 28 days was observed for the substance. Therefore, the substance is considered as not readily biodegradable.
Reference
The test substance is not toxic to bacteria.
Description of key information
With 0 % degradation within 28 days the substance is considered as not readily biodegradable.
Key value for chemical safety assessment
- Biodegradation in water:
- under test conditions no biodegradation observed
Additional information
Using the EU Method C.4-E (Determination of the "Ready" Biodegradability - Closed Bottle Test) 0 % degradation within 28 days was observed for the substance. Therefore, the substance is considered as not readily biodegradable.
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