3-chloroaniline

Repeated dose toxicity: inhalation

Administrative data

Endpoint:

short-term repeated dose toxicity: inhalation

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

4 (not assignable)

Rationale for reliability incl. deficiencies:

other: secondary literature

Data source

Reference

Reference Type:

secondary source

Title:

Unnamed

Year:

1982

Materials and methods

Principles of method if other than guideline:

Groups of 16 mal Crl:CD rats were exposed head-only, 6 hrs/day, 5 days/week, for 2 weeks to either 6, 30 or 50 ppm( = ca. 31.8, 159 or 265 mg/m³) m-chloroaniline. A control group was simultaneously exposed to air only. Animals were evaluated for clinical and histopathological lesions following 10 exposures and after a 14-day recovery period.

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

3-chloroaniline

EC Number:

203-581-0

EC Name:

3-chloroaniline

Cas Number:

108-42-9

Molecular formula:

C6H6ClN

IUPAC Name:

3-chloroaniline

Test material form:

other: liquid

Details on test material:

no data

Test animals

Species:

rat

Strain:

Crj: CD(SD)

Sex:

male

Administration / exposure

Route of administration:

other: vapour/aerosol

Type of inhalation exposure:

head only

Vehicle:

air

Remarks on MMAD:

MMAD / GSD: no data

Analytical verification of doses or concentrations:

yes

Duration of treatment / exposure:

2 weeks

Frequency of treatment:

6 hrs/day, 5 days/week, for 2 weeks

Doses / concentrations

Remarks:

Doses / Concentrations:
6, 30 or 50 ppm( = ca. 31.8, 159 or 265 mg/m³) m-chloroaniline
Basis:
nominal conc.

No. of animals per sex per dose:

16 animals/dose

Control animals:

yes, concurrent vehicle

Results and discussion

Effect levels

Dose descriptor:

LOAEC

Effect level:

32 mg/L air (nominal)

Based on:

test mat.

Sex:

male

Basis for effect level:

other: extramedullary hematopoesis, hemosiderin deposits in spleen, methemoglobinemia

Target system / organ toxicity

Critical effects observed:

not specified

Any other information on results incl. tables

During and immediately following exposure, rats in the 30 and 50 ppm dose groups were slight to moderately cyanotic. Slight increase in body weights were observed in all test groups during exposure and recovery.

Clinical pathological examinations revealed a dose-dependent effect of m-chloroaniline on several hematologic parameters. Decrease in erythrocyte count, hemoglobin content, and hematocrit and increase in leucocytes, immature erythrocytes, mean cell volume and mean cell hemoglobin resulted from 10 exposures to all exposure levels.

Pathologic examinations revealed dose-dependent microscopic changes in hematopoetic tissues consistent with the development of hemolytic anemia and subsequent extramodullary hematopoesis.

Applicant's summary and conclusion

Executive summary:

Groups of 16 mal Crl:CD rats were exposed head-only, 6 hrs/day, 5 days/week, for 2 weeks to either 6, 30 or 50 ppm( = ca. 31.8, 159 or 265 mg/m³) m-chloroaniline. A control group was simultaneously exposed to air only. animals were evaluated for clinical and histopathological lesions following 10 exposures and after a 14-day recovery period.

During and immediately following exposure, rats in the 30 and 50 ppm dose groups were slight to moderately cyanotic. Slight increase in body weights were observed in all test groups during exposure and recovery.

Clinical pathological examinations revealed a dose-dependent effect of m-chloroaniline on several hematologic parameters. Decrease in erythrocyte count, hemoglobin content, and hematocrit and increase in leucocytes, immature erythrocytes, mean cell volume and mean cell hemoglobin resulted from 10 exposures to all exposure levels.

Pathologic examinations revealed dose-dependent microscopic changes in hematopoetic tissues consistent with the development of hemolytic anemia and subsequent extramodullary hematopoesis.