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EC number: 205-592-6 | CAS number: 143-22-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Skin sensitisation
Administrative data
- Endpoint:
- skin sensitisation: in vivo (non-LLNA)
- Type of information:
- migrated information: read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- weight of evidence
- Study period:
- 1977
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Study conducted prior to GLP, but follows accepted guidelines and is well reported.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 002
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 406 (Skin Sensitisation)
- Deviations:
- no
- GLP compliance:
- no
- Type of study:
- guinea pig maximisation test
Test material
- Reference substance name:
- Shell DOT 4 Brake Fluid
- IUPAC Name:
- Shell DOT 4 Brake Fluid
- Details on test material:
- Composition (Chemical name,CAS No., and Percent Composition)
Contains:
Triethylene glycol monomethyl ether borate ester (30989-05-0) (30-50%);
Triethylene glycol monobutyl ether borate ester (3-30%);
Triethylene glycol monomethyl ether (112-35-6)(20-30%);
Triethylene glycol monobutyl ether (143-22-6)(2-10%);
Tetraethylene glycol monomethyl ether (23783-42-8) (20-30%),;
Tetraethylene glycol monobutyl ether (1559-34-8)(2-10%); minor additives (<1%).
Molecular Formula Mixture
CAS No. n/a
Physical form of marketed product Liquid
Melting point (oC) <51.7
Boiling point (oC) >260
Density (g/cm3) 1.06
Vapor pressure (hPa) < 0.01
Water solubility(mg/l ) Soluble
Flash point (oC) 121
Constituent 1
In vivo test system
Test animals
- Species:
- guinea pig
- Strain:
- other: P-strain
- Sex:
- male/female
Study design: in vivo (non-LLNA)
Inductionopen allclose all
- Route:
- intradermal and epicutaneous
- Vehicle:
- water
- Concentration / amount:
- 5% for induction phase 1, undiluted for induction phase 2, and 50% challenge phase
Challengeopen allclose all
- Route:
- epicutaneous, occlusive
- Vehicle:
- water
- Concentration / amount:
- 5% for induction phase 1, undiluted for induction phase 2, and 50% challenge phase
- No. of animals per dose:
- 20 (test material); 10 (negative controls)
- Details on study design:
- Induction Phase:
Guinea pigs were shorn in the shoulder region and challenged in 2 rows of 3 intradermal injections each with 0.1 mL Freund's Complete Adjuvant, 5% Brake Fluid 500 DOT 4 in water, or 5% Brake Fluid 500 DOT 4 in 50:50 water: FCA. One week after the intradermal injections, the site was clipped free of hair and a soaked filter paper, covered with overlapping plastic adhesive tape and secured with an elastic bandage and left in place for 48 hours.
Challenge Phase:
Two weeks after topical induction, hair was removed from the site and test material was applied via soaked filter paper, covered with an adhesive tape, and held in place with an elastic bandage for 48 hours. Reactions were read at 24 and 48 hours. - Challenge controls:
- Vehicle (water) was used as negative control
- Positive control substance(s):
- not required
Results and discussion
In vivo (non-LLNA)
Resultsopen allclose all
- Reading:
- 1st reading
- Hours after challenge:
- 24
- Group:
- negative control
- Dose level:
- 50%
- No. with + reactions:
- 9
- Total no. in group:
- 10
- Remarks on result:
- other: Reading: 1st reading. . Hours after challenge: 24.0. Group: negative control. Dose level: 50%. No with. + reactions: 9.0. Total no. in groups: 10.0.
- Reading:
- 2nd reading
- Hours after challenge:
- 48
- Group:
- negative control
- Dose level:
- 50%
- No. with + reactions:
- 0
- Total no. in group:
- 10
- Remarks on result:
- other: Reading: 2nd reading. . Hours after challenge: 48.0. Group: negative control. Dose level: 50%. No with. + reactions: 0.0. Total no. in groups: 10.0.
- Reading:
- 1st reading
- Hours after challenge:
- 24
- Group:
- test chemical
- Dose level:
- 50%
- No. with + reactions:
- 0
- Total no. in group:
- 19
- Remarks on result:
- other: Reading: 1st reading. . Hours after challenge: 24.0. Group: test group. Dose level: 50%. No with. + reactions: 0.0. Total no. in groups: 19.0.
- Reading:
- 2nd reading
- Hours after challenge:
- 48
- Group:
- test chemical
- Dose level:
- 50%
- No. with + reactions:
- 0
- Total no. in group:
- 19
- Remarks on result:
- other: Reading: 2nd reading. . Hours after challenge: 48.0. Group: test group. Dose level: 50%. No with. + reactions: 0.0. Total no. in groups: 19.0.
Applicant's summary and conclusion
- Interpretation of results:
- not sensitising
- Remarks:
- Migrated information Criteria used for interpretation of results: EU
- Conclusions:
- Brake Fluid 500 DOT 4 was non-sensitising to Guinea pig skin
- Executive summary:
Brake Fluid 500 DOT 4 was tested for skin sensitisation using the Guinea pig Magnusson and Kligman method. No evidence of skin sensitisation was seen in the study.
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