(methoxymethyl)triphenylphosphonium chloride

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2003

Report date:

2003

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

(methoxymethyl)triphenylphosphonium chloride

EC Number:

223-664-5

EC Name:

(methoxymethyl)triphenylphosphonium chloride

Cas Number:

4009-98-7

Molecular formula:

C20H20OP.Cl

IUPAC Name:

(methoxymethyl)triphenylphosphanium chloride

Test material form:

solid: particulate/powder

Remarks:

migrated information: powder

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

During the final test samples for analysis were taken from 0.32, 1.0 and 3.2 mg/I. Also samples were taken from the control. The method of analysis isdescribed in the appended Analytical Report.
Sampling:
Frequency: at 24 hours and 48 hours
Volume: 10 ml from the approximate centre of the test vessel
Storage: The samples were stored in a freezer until analysis

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

Start of each batch: With new-born animals, Le. less than 3 days old, by placing about 250 of them into 10 litres of medium in an all-glass culture
vessel.
Maximum age of the cultures: 4 weeks
Renewal of the cultures: After 7 days of cultivation half of the medium twice a week.
Temperature of medium: 18-22°C, constant within ± 1°C
Feeding: Daily, a suspension of fresh water algae.

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Test temperature:

19.7-20.5°C

pH:

7.7-7.9

Dissolved oxygen:

8.0-9.0 mg O2/L

Nominal and measured concentrations:

nominal 0.32, 0.56, 1.0, 1.8 and 3.2 mg/L

Reference substance (positive control):

yes

Remarks:

potassium dichromate

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

3.2 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: 95% CL: 2.7-4.8 mg/l

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

1.5 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: 95% CL: 1.2-1.9 mg/l

Effect concentrations 3

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

0.56 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Any other information on results incl. tables

Analysis of the samples taken showed that the measured concentrations were in agreement with nominal (98 -107%).

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The 48h-EC50 was 1.5 mg/l with a 95% confidence intervall between 1.2 and 1.9 mg/l.