Reaction mass of butyl diphenyl phosphate and dibutyl phenyl phosphate and tributyl phosphate

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

weight of evidence

Study period:

1979

Reliability:

4 (not assignable)

Rationale for reliability incl. deficiencies:

other: The study protocol is poorly documented.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1979

Report date:

1979

Materials and methods

Test guideline

Qualifier:

no guideline followed

Principles of method if other than guideline:

Acute oral toxicity test. A single oral dose was administered to rats. Animals were observed until 14 days after administration of the test substance. Mortalities, body weights and clinical observations were recorded.

GLP compliance:

no

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

Acryloid HF-422 (DBPP + polymer)

IUPAC Name:

Acryloid HF-422 (DBPP + polymer)

Constituent 2

Reference substance name:

Dibutyl phenyl phosphate

EC Number:

219-772-7

EC Name:

Dibutyl phenyl phosphate

Cas Number:

2528-36-1

Molecular formula:

C14H23O4P

IUPAC Name:

dibutyl phenyl phosphate

Details on test material:

Acryloid HF-422 (70.8% dibutylphenylphosphate and 29.2% n-butylmethacrylate polymer)

Test animals

Species:

rat

Strain:

Sprague-Dawley

Sex:

male/female

Administration / exposure

Route of administration:

oral: unspecified

Vehicle:

unchanged (no vehicle)

Doses:

500, 1500, 2500, 3500, 5000 mg/kg

No. of animals per sex per dose:

5 males and 5 females

Control animals:

not specified

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LD50

Effect level:

4 307 mg/kg bw

95% CL:

3 568 - 6 423

Any other information on results incl. tables

One animal at the 2500 mg/kg dose and one at the 5000 mg/kg dose exhibited alopecia. One animal at the 2500 mg/kg dose, two at the 2500 mg/kg dose, two at the 3500 mg/kg dose, and 8 at the 5000 mg/kg dose were hypoactive. Bloody appearing urine was seen in one animal at the 3500 mg/kg dose and in 2 at the 5000 mg/kg dose. One animal at the 3500 mg/kg dose and one at the 5000 mg/kg dose had ptosis. Ataxia was seen in one animal at the 3500 mg/kg dose and in 5 animals at the 5000 mg/kg dose. Excessive lacrimation was observed in one animal at the 3500 mg/kg dose and in 4 animals at the 5000 mg/kg dose.

Gross necropsy: 20 animals were necropsied at terminal sacrifice. One had very pale lungs. One had very pale colored kidneys. One had unilateral hydronephrosis and white sediment in the same kidney. The remaining 17 animals were not remarkable.

Dose	Body weight (Gm)			Mortalities / dosed
mg/kg	Day 0 M/F	Day 7 M/F	Day 14 M/F	Male	Female	Combined
500	176/173	251/213	300/228	0/5	0/5	0/10
1500	171/171	244/218	293/227	0/5	0/5	0/10
2500	178/178	246/222	297/242	0/5	1/5	1/10
3500	178/168	245/203	307/221	0/5	2/5	2/10
5000	172/171	215/ -	281/ -	2/5	5/5	7/10