Aluminium metaphosphate

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

03.11.2014 - 09.01.2015

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Qualifier:

according to guideline

Guideline:

EU Method C.1 (Acute Toxicity for Fish)

GLP compliance:

yes (incl. QA statement)

Remarks:

Ministerium für Arbeit, Integration und Soziales des Landes Nordrhein- Westfalen

Analytical monitoring:

yes

Details on sampling:

- Concentrations: 6.25, 12.5, 25, 50, and 100 mg/L
- Sampling method: Samples were taken directly after test solution preparation and at each renewal in fresh medium and at the respective end of the incubation period (48 hours).
- Sample storage conditions before analysis: Samples were stabelised by acidification and analysed after max. 11 days.

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Preparation of WAF according to OECD guidedance No. 23 and 29.
The test item was added to the dilution water and stirred for 24 hours at ca. 20 °C. The solution was allowed to stand for 2 hours and subsequently insoluble parts were separated from the aqueous phase by filtration (0.22 μm PES membrane). The test solution were obtained by dilution of the highest test concentration.The test solutions were freshly prepared before test start and renewal of test solution.

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name: zebra fish
- Source: Test facility bred
- Length at study initiation (length definition, mean, range and SD): 2.0 – 2.3 cm
- Weight at study initiation (mean and range, SD): 0.07 - 0.10 g
- Feeding during test: no

ACCLIMATION
- Acclimation period: ≥12 days
- Acclimation conditions (same as test or not): same as test conditions
- Type and amount of food: brine shrimp (Artemia spp.) nauplii and ground flake food TetraMinR (Tetra Werke, Melle, Germany)
- Feeding frequency: daily
- Health during acclimation (any mortality observed): < 5% one week before test start

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Hardness:

1.3 mmol/L

Test temperature:

22.8 – 22.9 °C

pH:

8.5 – 8.8

Dissolved oxygen:

94 - 98 %

Nominal and measured concentrations:

Nominal substance concentration: 6.25, 12.5, 25, 50, and 100 mg/L
The nominal substance concentrations correspond to: 0.72, 1.44, 2.89, 5.77 and 11.54 mg Al/L
Mean measured test substance concentrations: 0.0126, 0.0238, 0.0607, 0.0905, 0.1557 mg Al/L

Details on test conditions:

TEST SYSTEM
- Test vessel: glass aquaria
- Material, size, fill volume: glass, 10 L nominal volume, 10 L fill volume
- Aeration: slightly aeration via a glass capillary
- Renewal rate of test solution (frequency): every 48 hours
- No. of organisms per vessel: 7
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: 0.06 g/L

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: purified drinking water according to the OECD-Guideline
- Alkalinity: 2.2 mmol/L
- Conductivity: 188 μS/cm

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 12/12 hours day-night regime

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Mortality and abnormal behaviour after 3 h, 24 h, 48 h, 72 h and 96 h.

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC10

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC10

Effect conc.:

> 0.156 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

other: mg Al/L

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 0.156 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

other: mg Al/L

Basis for effect:

mortality (fish)

Details on results:

- Behavioural abnormalities: no
- Observations on body length and weight: no
- Other biological observations: no
- Mortality of control: no
- Abnormal responses: no

Reported statistics and error estimates:

Statistical calculations were performed using the computer programme ToxRat®

Sublethal observations / clinical signs:

Table 1: Measured concentrations of the test item considering background values of controls. 

Al = Aluminium. Measurement based on total aluminium.

Nominal concentration	0 h	%	48 h aged	%	48 h fresh	%	96 h aged	%	Mean	%
µg Al/L	µg Al/L	initial	µg Al/L	initial	µg Al/L	initial	µg Al/L	initial	µg Al/L	initial
721	12.35	1.7	11.78	1.6	12.18	1.7	14.00	1.9	12.58	1.7
1443	24.67	1.7	24.16	1.7	24.42	1.7	21.77	1.5	23.76	1.6
2885	56.89	2.0	59.20	2.1	62.13	2.2	64.39	2.2	60.66	2.1
5770	88.37	1.5	92.42	1.6	87.96	1.5	93.37	1.6	90.53	1.6
11540	148.48	1.3	160.38	1.4	152.12	1.3	161.89	1.4	155.72	1.3

LOD (Limit of detection) = 0.169 µg/L
LOQ (Limit of quantification) = 0.507 µg/L

Table2: Survived fish during the test period of 96 h (number of fish per treatment )

Al = Aluminium; Concentrations given as mean measured values.

Incubation	Control	0.013 mg Al per L	0.024 mg Al per L	0.061 mg Al per L	0.091 mg Al per L	0.156 mg Al per L
3 h	7	7	7	7	7	7
24 h	7	7	7	7	7	7
48 h	7	7	7	7	7	7
72 h	7	7	7	7	7	7
96 h	7	7	7	7	7	7

 

Table3: Mortality [%] during the test period of 96 h.

 Al = Aluminium; Concentrations given as mean measured values.

Incubation	Control	0.013 mg Al per L	0.024 mg Al per L	0.061 mg Al per L	0.091 mg Al per L	0.156 mg Al per L
3 h	0	0	0	0	0	0
24 h	0	0	0	0	0	0
48 h	0	0	0	0	0	0
72 h	0	0	0	0	0	0
96 h	0	0	0	0	0	0

 

Validity criteria fulfilled:

yes

Description of key information

EC50 (96h) >  100mg/L (nominal)

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

100 mg/L

Additional information

One short-term study with aluminium metaphosphate (CAS 13776-88-0) according to OECD guideline 203 and GLP was performed with Brachydanio rerio (Simon, 2014). The test was conducted under semi-static conditions for 96 h. Since the test item has a low water solubility, the test media were prepared using the Water Accommodated Faction (WAF) approach according to the OECD guidance documents No. 23 and 29 to obtain the maximal solubility of the test item under the test conditions. There were no concentration-dependent effects on survival up to the highest test concentrations of 0.156 mg aluminium per liter (100 mg/L nominal test substance). Thus the substance is considered not acutely toxic to fish.