Benzoic acid, 4-[(1-oxodecyl)oxy]-

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Study performed according to current OECD-Guideline, well documentde but without GLP

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2004

Report date:

2004

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

Decanoyloxybenzoic acid

IUPAC Name:

Decanoyloxybenzoic acid

Constituent 2

Reference substance name:

SEEAUS 035

IUPAC Name:

SEEAUS 035

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

sewage, predominantly domestic, non-adapted

Details on inoculum:

Activated sludge from sewage treatment plant FrankfurtNiederrad

Duration of test (contact time):

28 d

Initial test substance concentration

Initial test substance concentration 1

Initial conc.:

16.6 mg/L

Based on:

test mat.

Initial test substance concentration 2

Initial conc.:

16.8 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

CO2 evolution

Reference substance

Reference substance:

benzoic acid, sodium salt

Results and discussion

% Degradation

Parameter:

% degradation (CO2 evolution)

Value:

80

Sampling time:

28 d

Details on results:

The test substance Decanoyloxybenzoic acid in the concentration of 24 mg/I reached a degree of degradation of 80 % after 28 days. The level of 10 % degradation (begin of biodegradation) was reached after 1 day. The 60 % level was passed after 7 days. The pass level for ready biodegradability, i. e. ~ 60 % theoretical'C02 production within 10 days after the degree ofdegradation has reached 10 % (l0 d window), was reached. The test substance is readily biodegradable.
In order to check the validity ofthe test sodium benzoate in a concentration of 20 mg/l was used as reference substance. The pass level of60 % degradation within '14 days was reached. In a toxicity test, containing both the test substance and the reference substance, the degradation reached 82 %. The test substance can be assumed to be not inhibitory

Any other information on results incl. tables

Degree of degradation of the test substance (S1: 24.3 mg/l, S2: 23.9 mg/l)

 

                                       Degr. [%]

Study day (d)              Sl               S2

2                               22              21

5                               45              43

7                               62              59

9                              71              69

12                            76              76

16                            78              78

21                             79              80

28                            79              81

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

readily biodegradable

Conclusions:

The test substance Decanoyloxybenzoic acid in the concentration of 24 mg/I reached a degree of degradation of 80 % after 28 days. The level of 10 % degradation (begin of biodegradation) was reached after 1 day. The 60 % level was passed after 7 days. The test substance is readily biodegradable.

Executive summary:

The ready biodegradability ofthe test substance Decanoyloxybenzoic acid was tested in the CO2 Evolution Test with non-adapted inoculum over a period of 28 days according to DECD Guideline for Testing of Chemicals 301 B. The test substance Decanoyloxybenzoic acid in the concentration of 24 mg/I reached a degree of degradation of 80 % after 28 days. The level of 10 % degradation (begin of biodegradation) was reached after 1 day. The 60 % level was passed after 7 days. The pass level for ready biodegradability, i. e. ~ 60 % theoretical'C02 production within 10 days after the degree ofdegradation has reached 10 % (l0 d window), was reached. The test substance is readily biodegradable.

In order to check the validity ofthe test sodium benzoate in a concentration of 20 mg/l was used as reference substance. The pass level of 60 % degradation within '14 days was reached. In a toxicity test, containing both the test substance and the reference substance, the degradation reached 82 %. The test substance can be assumed to be not inhibitory. The validity criteria according to the guideline were fulfilled in the test.