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EC number: 400-410-3 | CAS number: 25485-88-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Bioaccumulation: aquatic / sediment
Administrative data
- Endpoint:
- bioaccumulation in aquatic species: fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1993
- Reliability:
- 2 (reliable with restrictions)
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 993
- Report date:
- 1993
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 305 E (Bioaccumulation: Flow-through Fish Test)
- GLP compliance:
- yes
Test material
- Reference substance name:
- -
- EC Number:
- 400-410-3
- EC Name:
- -
- Cas Number:
- 25485-88-5
- Molecular formula:
- C13H16O3
- IUPAC Name:
- cyclohexyl 2-hydroxybenzoate
- Details on test material:
- Carboxyl labelled 14C, Batch No. 92301, specific activity 9.41 MBq/mg, dissolved in Ethanol with Specific Activity 16.7 MBq/mL, Radiopurity > 98%.
Constituent 1
- Radiolabelling:
- yes
Test solutions
- Vehicle:
- yes
Test organisms
- Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
Study design
- Route of exposure:
- aqueous
- Test type:
- flow-through
- Water / sediment media type:
- natural water: freshwater
- Total exposure / uptake duration:
- 28 d
- Total depuration duration:
- 14 d
Test conditions
- Nominal and measured concentrations:
- At 1 ug/L test concentration, the actual measured concentration was determined to be 1.05 ug/L.
At 10 ug/L test concentration, the actual measured concentration was determined to be 8.5 ug/L.
Results and discussion
Bioaccumulation factoropen allclose all
- Type:
- BCF
- Value:
- 600 - 900 L/kg
- Basis:
- whole body w.w.
- Time of plateau:
- 2 d
- Calculation basis:
- steady state
- Remarks on result:
- other: Conc.in environment / dose:1microg/L
- Type:
- BCF
- Value:
- 600 - 900 L/kg
- Basis:
- whole body w.w.
- Time of plateau:
- 2 d
- Calculation basis:
- steady state
- Remarks on result:
- other: Conc.in environment / dose:10microg/L
Depurationopen allclose all
- Elimination:
- yes
- Parameter:
- DT50
- Depuration time (DT):
- 3 d
- Parameter:
- DT90
- Depuration time (DT):
- 5 d
Any other information on results incl. tables
1mg/L exposure level– Actual measured concentration was 1.05mg/L. At least half- to ¾ of the total radioactivity recovered via extraction was in the form of the Parent material. The BCF based on CYCLOHEXYL SALICYLATE was calculated to be between 600 and 900 L/kg (wet wt.). Steady state was attained within the first 2 Days of exposure. > 50% of the accumulated radioactivity had dissipated within the first 2- to 3-days of the depuration phase (Ct50 < 3 days and Ct90 < 5 days).10mg/L exposure level- Actual measured concentration was 8.5mg/L. At least half- to ¾ of the total radioactivity recovered via extraction was in the form of the Parent material. The BCF based on CYCLOHEXYL SALICYLATE was calculated to be between 600 and 900 L/kg (wet wt.). Steady state was attained within the first 2 Days of exposure. > 50% of the accumulated radioactivity had dissipated within the first 2- to 3-days of the depuration phase (Ct50 < 3 days) .
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Executive summary:
The potential of CYCLOHEXYL SALICYLATE to bioaccumulate in fish was studied using C-14 (carboxyl) labelled test substance at two test concentrations using Zebrafish (Brachydanio rerio). The fish were acclimated in drinking water for at least 2 Weeks at a temperature of 23 °C, and the validity of the fish stock verified. Prior to the start of the exposure phase, lipid content was determined from 15 fish.
Uptake Phase– The study was conducted as a flow-through test at 23 °C in filtered drinking water. Fish were exposed in 6 Litre tanks. Ten volume exchanges were performed per day. Oxygen concentration was > 60% of saturation. Two exposure concentrations were performed : 1mg/L and 10mg/L. Fish samples were taken at regular intervals (Day0, 2, 6, 12, 20 and 28), left to drip dry, euthanised and combusted to determine total C-14 content via14CO2. At the end of the uptake phase (Day 28), 15 fish were sampled from the 1mg/L and extracted with ethanol to determine test material. There was a > 20% variation in the test material concentration in the test vessels during the first 2 Days of the uptake phase presumably due to strong adsorption of the test material.
Depuration Phase– Fish were transferred from the treated aqueous phase to clean water containing no test substance, and, incubated for a period of 14 Days. Fish were removed for analysis on Day 30, 33, 37 and 42. The lipid content was determined on fish from the 10mg/L treatment level at the end of the depuration phase of the study.
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