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EC number: 601-093-6 | CAS number: 111453-32-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
- Endpoint:
- toxicity to microorganisms, other
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- July 1992
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 993
- Report date:
- 1993
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- DIN 38412-8 (Pseudomonas Zellvermehrungshemmtest)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- rac-5-Amino-N-(2,3-dihydroxypropyl)-2,4,6-triiodoisophthalamic acid
- EC Number:
- 601-093-6
- Cas Number:
- 111453-32-8
- Molecular formula:
- C11 H11 I3 N2 O5
- IUPAC Name:
- rac-5-Amino-N-(2,3-dihydroxypropyl)-2,4,6-triiodoisophthalamic acid
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
12500 mg of ZK 39.211 were dissolved in 1000 ml bidistilled water. The pH was 2.2 and was adjusted with 1 mol NaOH to 7.6. Then the further dilutions were prepared:
for the 1st: 100 mL of stock solution + 900 mL of bidist. water
for the 2nd: 10 mL of stock solution + 990 mL of bidist. water
for the 3rd: 1 mL of stock solution + 999 mL of bidist. water
for the 4th: 0.1 mL of stock solution + 999.9 mL of bidist. water
for the 5th: 0.01 mL of stock solution + 999.99 mL of bidist. Water
80 mL of the respective solution were filled into the respective test vessel.
Test organisms
- Test organisms (species):
- Pseudomonas putida
- Details on inoculum:
- - Laboratory culture: Schering AG
- Name and location of sewage treatment plant where inoculum was collected: The bacterium was obtained from the "Deutsche Sammlung für Mikroorganismen, Göttingen"
- Method of cultivation: kept on slant agar containing a nutrient solution at approximately 25°C. During culturing the microorganisms were transferred weekly.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 16 h
Test conditions
- Test temperature:
- room temperature
- Details on test conditions:
- TEST SYSTEM
- Aeration: shaking
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2
- Test concentrations: 0.1, 1.0, 10, 100, 1000 and 10000 mg/L - Reference substance (positive control):
- no
Results and discussion
Effect concentrations
- Key result
- Duration:
- 16 h
- Dose descriptor:
- EC10
- Effect conc.:
- > 10 g/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth inhibition
- Details on results:
- The test substance was incubated with bacteria Pseudomonas putida over aperiod of 16 h. The test concentrations were 0, 0.1, 1, 10, 100, 1000, 10000 g/L. The bacterial growth was measured on the basis of a turbidity analysis by means of a spectrophotometer. None of the concentration results differed from those of the controls. Thus, there was no indication that the testcompound affected bacterial growth.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The EC50 (16 h) was higher than the highest concentration (10 g/L). Therefore, the EC10/EC50 was higher than 10 g/L.
- Executive summary:
The test substance was incubated with bacteria Pseudomonas putida over a period of 16 h. The test concentrations were 0, 0.1, 1, 10, 100, 1000, 10000 g/L.The bacterial growth was measured on the basis of a turbidity analysis by means of a spectrophotometer. None of the concentration results differed from those of the controls. Thus, there was no indication that the testcompound affected bactrila growth.
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