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EC number: - | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- effects on growth of green algae
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- February 28, 2019 to April 10, 2019
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 019
- Report date:
- 2019
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: HJ/T 153-2004, "The Guidelines for the Testing of Chemicals" of " No. 201 'Alga Growth Inhibition Test'" (Second edition)
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Sulfonic Acids, C18-alkane hydroxyl and C18-alkene, potassium salts
- Cas Number:
- 2210238-56-3
- IUPAC Name:
- Sulfonic Acids, C18-alkane hydroxyl and C18-alkene, potassium salts
- Test material form:
- cream / paste
- Remarks:
- Pale yellow paste at 50℃, This sample was obtained from the original liquid form by removing water.
Constituent 1
- Specific details on test material used for the study:
- Storage Condition: Store container tightly closed in well-ventilated place. Store in corrosive resistant/container with a resistant in liner. Store locked up. Don ' t store with acids.
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- Took 1.0 mL of 25, 50 and 100 mg a.i./L test solution in 10 mL volumetric flask at 0, 24, 48 and 72h, dilute with deionized water to scale, blending, and took 1.0 mL those above solution into a 100 mL volumetric flask, dilute with deionized water to scale, took test solution after blending, measured its concentration under instrument condition. Took 1.0 mL of 200, 400 and 800 mg a.i./L test solution in 50 mL volumetric flask at 0,24, 48 and 72h, dilute with deionized water to scale, blending, and took 1.0 mL those above solution into 100 mL volumetric flask, dilute with deionized water to scale, took test solution after blending, measured its concentration under instrument condition.
Test solutions
- Vehicle:
- no
- Details on test solutions:
- Culture medium was prepared with deionized water ; the final concentration of each nutritive salt in culture medium was shown as the following:
Alimentation salt Concentration
NH4Cl 15 mg a.i./L
MgCl2·6H2O 12 mg a.i./L
CaCl2-2H2O 18 mg a.i./L
MgS04·7H2O 15 mg a.i./L
KH2PO4 1.6 mg a.i./L
FeCl3·6H2O 80 µg/L
Na2EDTA·2H2O 100 µg/L
H3BO3 185 µg/L
MnCl2-4H2O 415 µg/L
ZnCl2 3 µg/L
CoCl2·6H2O 1.5 µg/L
CuCl2·2H2O 0.01 µg/L
NaMoO4·2H2O 7 µg/L
NaHCO3 50 mg a.i./L
Test organisms
- Test organisms (species):
- Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- The test organisms
Selenastrum capricornutum were obtained from Freshwater Algae Culture Collection at the Institute of Hydrobiology in April 2018 and were cultivated in the laboratory.
Preliminary culture
Took a certain amount of Selenastrum capricornutum from stock culture, inoculated them in fresh and aseptic culture medium, and cultivated them under the same condition as test requirements. Selenastrum capricornutum would be inoculated in the fresh and aseptic culture medium once every 2~4 days, duplicated the same test for 2~3 times. Inspected that Selenastrum capricornutum were growing well and under logarithm growth period, thus the algae test solution was prepared for using.
The serial numbers of this test organisms as follow: S20190301090
Study design
- Test type:
- static
- Water media type:
- other: deionized water
- Limit test:
- no
- Total exposure duration:
- 72 h
Test conditions
- Test temperature:
- 21~24℃
- pH:
- 7.41~8.36
- Nominal and measured concentrations:
- Nominal concentrations: 25, 50, 100, 200, 400, 800 mg a.i./L
Measured concentrations 0h: 28.83, 53.71, 107.65, 209.45, 426.9, 869.9 mg a.i./L
Measured concentrations 24h: 26.06, 49.96, 103.43, 207.8, 398.4, 881.4 mg a.i./L
Measured concentrations 48h: 23.86, 48.65, 101.81, 187.0, 354.8, 752.65 mg a.i./L
Measured concentrations 72h: 23.77, 47.38, 93.00, 189.05, 355.45, 762.85 mg a.i./L
Geometric mean concentrations: 25.56, 49.87, 101.33, 198.06, 382.69, 814.55 mg a.i./L - Details on test conditions:
The nominal concentrations such as 1, 10, 100, 200 and 400 mg a.i./L of C18K test solution and blank control were prepared in preliminary test. The static test method was selected to perform preliminary test, the test period was 72h. Observed and recorded the growth of Selenastrum capricornutum in test solution of 24h, 48h and 72h. Illumination intensity and pH values of the test solutions were determined at 0h, 72h. Took a bottle of test solution into the incubator which as same as blank group to record the temperature every day. The temperature of the cup of test solution was between 22.6 and 23.5℃.
-Preparation of testing solution
Weighed 2.5575 g test substance and put it in a 100 mL beaker, added test culture medium to dissolved and transferred it to a 500 mL volumetric flask, and made constant volume to the scale with test medium, took ultrasound for 10 min, obtain the stock solution with concentration of 2000 mg a.i./L, diluted the stock solution with test medium solution to a concentration twice that of the needed test solution. The preparation of test solutions was showed in table 1.
-Operating of test
According to the preliminary test results, the nominal concentrations such as 25, 50, 100, 200, 400 and 800 mg a.i./L test solution of C18K was prepared to perform definitive test, every concentration set three parallel and a blank control. The static test method was selected to perform definitive test, the test period was 72h. Observed and recorded the growth of Selenastrum capricornutum in test solution of 24h, 48h and 72h. Illumination intensity, pH values and the actual concentration of the test solutions were determined at 0h, 72h. Took a bottle of test solution into the incubator which as same as blank group to record the temperature every day. The temperature of the cup of test solution was between 22.4 and 23.3℃.
(1) Since the initial of the test, the concentration of the alga cells increased up to 3.83 x 106 cells/mL from 2 x I04 cells/mL, increased by 192 times in 72h.
(2) The mean coefficient of variation for section-by-section specific growth rates (days 0-1, 1-2 and 2-3, for 72-hour tests) in the control cultures was less than 35%.
(3) In replicate control cultures , the coefficient of variation of average specific growth rates was less than or equal to 7% during the whole test period.
-Percent inhibition of growth rate
Calculate the percent inhibition of growth rate for each treatment replicate from equation [1]:
Ir = ( μc - μt ) / μc × 100・・・・・・[1]
Where:
Ir: Percent inhibition in average specific growth rate;
μc: Mean value for average specific growth rate (µ) in the control group;
µt: Average specific growth rate for the treatment replicate.
The average specific growth rate for a specific period is calculated as the logarithmic increase in the biomass from the equation for each single vessel of controls and treatments [2]:
µi-j = ( InXj - InXi) / ( tj - ti ) ・・・・・・[2]
Where:
μi-j: The average specific growth rate from time i to j,
Xi: The biomass at time i,
Xi: The biomass at time j
-Percent inhibition of yield
The percent inhibition in yield (Iy) may be calculated for each treatment replicate as follows:
Iy = ( Yc - Yt ) / Yc × 100・・・・・・[3]
Where:
ly: percent inhibition of yield,%,
Yc: mean value for yield in the control group,
Yt: value for yield for the treatment replicate .
-Estimates of ECx and confidence limits
The ECx and 95% confidence limits can be calculated by SPSS 16.0.
-LOEC and NOEC
For estimation of the LOEC and hence the NOEC (p < 0.05), for effects of the test substance on growth rate, it is necessary to compare treatment means using analysis of variance (ANOVA) techniques. Dunnett'c was used (One-Way ANOVA, SPSS 16.0).
Results and discussion
Effect concentrationsopen allclose all
- Key result
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 25.56 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- act. ingr.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- LOEC
- Effect conc.:
- 49.87 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- act. ingr.
- Basis for effect:
- growth rate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 589.6 mg/L
- 95% CI:
- 427.44 - 928.18
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- act. ingr.
- Basis for effect:
- growth rate
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 604.42 mg/L
- 95% CI:
- 436.24 - 959.75
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- act. ingr.
- Basis for effect:
- growth rate
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- 618.34 mg/L
- 95% CI:
- 446.02 - 983.37
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- act. ingr.
- Basis for effect:
- growth rate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 53.56 mg/L
- 95% CI:
- 18.95 - 93.26
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- act. ingr.
- Basis for effect:
- yield
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 93.34 mg/L
- 95% CI:
- 42.27 - 165.06
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- act. ingr.
- Basis for effect:
- yield
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- 335.61 mg/L
- 95% CI:
- 186.52 - 1 021.48
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- act. ingr.
- Basis for effect:
- yield
- Details on results:
The test results of definitive test were given in table 4, table 5 and table 6.
Growth curves of biomass versus time of treatment group results in definitive test and Concentration-Response Curves of definitive test were given in Figure 1 and Figure 2 in attached document.
Any other information on results incl. tables
Table 2 Results of preliminary test
Nominal concentration (mg a.i./L) | Density of algae cells (cells/mL) | Inhibition ratio (%) | ||||
Oh | 24h | 48h | 72h | 72h-Ir | 72h-Iy | |
0 | 20000 | 103000 | 541000 | 2890000 | - | - |
1 | 20000 | 107000 | 583000 | 3130000 | -1.6 | -8.3 |
10 | 20000 | 101000 | 515000 | 2620000 | 2.0 | 9.3 |
100 | 20000 | 66600 | 226000 | 783000 | 26.3 | 72.9 |
200 | 20000 | 58700 | 168000 | 474000 | 36.4 | 83.6 |
400 | 20000 | 58700 | 158000 | 419000 | 38.8 | 85.5 |
Table 3 Physical and chemical data results of preliminary test
Nominal Concentration (mg a.i./L) | pH values | Morphological observation of algae cells | |||
0h | 72h | 24h | 48h | 72h | |
0 | 8.05 | 7.92 | A | A | A |
1 | 8.03 | 7.45 | A | A | A |
10 | 8.03 | 7.42 | A | A | A |
100 | 8.07 | 7.72 | C | C | C |
200 | 8.14 | 7.82 | C | C | C |
400 | 8.47 | 7.94 | C | C | C |
A - Normal morphology B - Cells became small C - Paramorphia D - Color changes E - Adhesion aggregation |
Table 4 Control group Results of definitive test
Density of algae cells (cells/mL) | 0h (N) | 24h (N) | 48h (N) | 72h (N) |
20000 | 112000 | 639000 | 3520000 | |
20000 | 116000 | 668000 | 3850000 | |
20000 | 125000 | 724000 | 4120000 | |
The specific growth rate (day-1) | - | 1.723 | 1.741 | 1.706 |
1.758 | 1.751 | 1.752 | ||
1.833 | 1.756 | 1.739 | ||
The average specific growth rate of coefficient of variation (%) | 3.17 (0~1)d | 0.44 (1~2)d | 1.37 (2~3)d | |
The mean of coefficient of variation (%) | 1.66 | |||
The average specific growth rate of each parallel (%) | 1.723 (Repeat 1) | 1.754 (Repeat 2) | 1.776 (Repeat 3) | |
Coefficient of variation of the average specific growth rate (%) | 1.52 |
Table 5 Treatment group Results of definitive test
Nominal concentration (mg a.i./L) | Density of algae cells (cells/mL) | Inhibition ratio (%) | ||||||||
Oh(N) | 24h (N) | 48h(N) | 72h(N) | 24h-Ir | 48h-Ir | 72h-Ir | 24h-Iy | 48h-Iy | 72h-Iy | |
0 | 20000 | 112000 | 639000 | 3520000 | - | - | - | - | - | - |
20000 | 116000 | 668000 | 3850000 | |||||||
20000 | 125000 | 724000 | 4120000 | |||||||
25 | 20000 | 113000 | 591000 | 3190000 | 3.2 | 3.7 | 3.7 | 5.4 | 12.3 | 17.7 |
20000 | 103000 | 565000 | 2960000 | |||||||
20000 | 118000 | 626000 | 3310000 | |||||||
50 | 20000 | 91000 | 407000 | 1780000 | 15.7 | 16.1 | 16.1 | 24.3 | 43.4 | 57.1 |
20000 | 86100 | 368000 | 1540000 | |||||||
20000 | 90100 | 375000 | 1610000 | |||||||
100 | 20000 | 74400 | 271000 | 960000 | 25.9 | 26.3 | 26.6 | 36.8 | 60.2 | 75.3 |
20000 | 78300 | 271000 | 960000 | |||||||
20000 | 70500 | 237000 | 895000 | |||||||
200 | 20000 | 62600 | 195000 | 568000 | 35.6 | 35.9 | 36.7 | 46.8 | 71.6 | 85.5 |
20000 | 66600 | 212000 | 605000 | |||||||
20000 | 57800 | 169000 | 498000 | |||||||
400 | 20000 | 62600 | 197000 | 513000 | 39.1 | 39.2 | 39.4 | 49.9 | 74.7 | 87.4 |
20000 | 58700 | 168000 | 487000 | |||||||
20000 | 55400 | 148000 | 449000 | |||||||
800 | 20000 | 47500 | 113000 | 268000 | 51.6 | 52.4 | 52.7 | 59.9 | 84.2 | 93.7 |
20000 | 47000 | 98900 | 208000 | |||||||
20000 | 47000 | 109000 | 247000 |
Table 6 Physical and chemical data result s of definitive test
Nominal concentration (mg a.i./L) | pH values | Morphological observation of algae cells | |||
0h | 72h | 24h | 48h | 72h | |
0 | 7.93 | 7.44 | A | A | A |
25 | 7.98 | 7.41 | A | A | A |
50 | 8.06 | 7.46 | C | C | C |
100 | 8.14 | 7.64 | C | C | C |
200 | 8.23 | 7.82 | C | BC | BC |
400 | 8.33 | 7.89 | BC | BC | BC |
800 | 8.36 | 8.04 | BC | BC | BC |
A - Normal morphology B - Cells became small C – Paramorphia D - Color changes E - Adhesion aggregation |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The result of definitive test indicated that: Alga cells concentration of blank control increased by 192 times within 72h. The 24h-EyC50 of C18K to Selenastrum capricornutum was 335.61 mg a.i./L (actual concentration), 95% confidence limit was 186.52~1021.48 mg a.i./L; the 48h-EyC50 of C18K to Selenastrum capricornutum was 93.34 mg a.i./L (actual concentration), 95% confidence limit was 42.27~165.06 mg a.i./L; the 72h-EyC50 of C18K to Selenastrum capricornutum was 43.56 mg a.i./L (actual concentration), 95% confidence limit was 18.95~94.26 mg a.i./L;
The 24h-ErC50 of C18K to Selenastrum capricornutum was 618.34 mg a.i./L (actual concentration), 95% confidence limit was 446.02~983.37 mg a.i./L; the 48h-ErC50 of C18K to Selenastrum capricornutwn was 604.42 mg a.i./L (actual concentration), 95% confidence limit was 436.24~959.75 mg a.i./L; the 72h-ErC50 of C18K to Selenastrum capricornutum was 589.60 mg a.i./L (actual concentration), 95% confidence limit was 427.44.79~928.18 mg a.i./L.
The LOEC of C18K to Selenastrum capricornutum was less than 49.87 mg a.i./L (actual concentration), and the NOEC of C18K to Selenastrum capricornutum was greater than 25.56 mg a.i./L (actual concentration). - Executive summary:
The testing of the test item was carried out according to the test method "The Guidelines for the Testing of Chemicals" of "No.201 'Alga growth inhibition test"' (Second edition) and "OECD Guidelines for Testing of Chemicals No.201 'Alga Growth Inhibition Test"', According to the study plan and relevant SOP, Alga growth inhibition test of C18K was performed with static method, the test period was 72h.
According to the preliminary test results, the nominal concentrations such as 25, 50, 100, 200, 400 and 800 mg a.i./L test solution of C18K was prepared to perform definitive test, every concentration set three parallel and a blank control. Observed and recorded the growth of Selenastrum capricornutum in test solution of 24h, 48h and 72h. Illumination intensity, pH values and the actual concentration of the test solutions were determined at 0h, 72h. Took a bottle of test solution into the incubator which as same as blank group to record the temperature every day. The temperature of the cup of test solution was between 22.4 and 23.3℃.
The result of definitive test indicated that: Alga cells concentration of blank control increased by 192 times within 72h. The 24h-EyC50 of C18K to Selenastrum capricornutum was 335.61 mg a.i./L (actual concentration), 95% confidence limit was 186.52~1021.48 mg a.i./L; the 48h-EyC50 of C18K to Selenastrum capricornutum was 93.34 mg a.i./L (actual concentration), 95% confidence limit was 42.27~165.06 mg a.i./L; the 72h-EyC50 of C18K to Selenastrum capricornutum was 43.56 mg a.i./L (actual concentration), 95% confidence limit was 18.95~94.26 mg a.i./L;
The 24h-ErC50 of C18K to Selenastrum capricornutum was 618.34 mg a.i./L (actual concentration), 95% confidence limit was 446.02~983.37 mg a.i./L; the 48h-ErC50 of C18K to Selenastrum capricornutwn was 604.42 mg a.i./L (actual concentration), 95% confidence limit was 436.24~959.75 mg a.i./L; the 72h-ErC50 of C18K to Selenastrum capricornutum was 589.60 mg a.i./L (actual concentration), 95% confidence limit was 427.44.79~928.18 mg a.i./L.
The LOEC of C18K to Selenastrum capricornutum was less than 49.87 mg a.i./L (actual concentration), and the NOEC of C18K to Selenastrum capricornutum was greater than 25.56 mg a.i./L (actual concentration).
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