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EC number: 247-728-7 | CAS number: 26479-35-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2012-01-16 to 2012-11-13
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP study conducted per OECD 202
Cross-referenceopen allclose all
- Reason / purpose for cross-reference:
- reference to same study
- Reason / purpose for cross-reference:
- reference to other study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 012
- Report date:
- 2012
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- GLP compliance:
- yes
- Remarks:
- Only Exceptions: Lastest water characterization in Feb 2012 and test substance characterization not performed in accordance to GLP.
Test material
- Reference substance name:
- Potassium carbamoylcarbamate
- EC Number:
- 247-728-7
- EC Name:
- Potassium carbamoylcarbamate
- Cas Number:
- 26479-35-6
- Molecular formula:
- C2H3KN2O3
- IUPAC Name:
- potassium N-carbamoylcarbamate
- Test material form:
- solid: particulate/powder
- Remarks:
- migrated information: particulates
- Details on test material:
- - Name of test material (as cited in study report):Potassium Allophonate
- Physical state:powder
- Analytical purity:83.8%
1
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
N/A
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
Test solutions were sampled at 0 and 24 hours for fresh solution and 24 and 48 hours on spent solution. For each sampling a 10-mL sample was collected and diluted as necessary with 80:20 acetonitrile:water to provide final concentrations within the analytical standard range of 0.0250 to 1.25 ug a.i./mL. Analytical standards were prepared by diluting volumes of primary stock (1.15 mg a.i./mL) with 80:20 acetonitrile:HPLC water.
Test solutions
- Vehicle:
- no
- Details on test solutions:
- A primary standard of .10 mg a.i./L was prepared by diluting 0.237 g (0.2000 adjusted for purity) potassium allophonate to a volume of 1000mL. This standard was used as the highest concentration. Appropriate volumes of the primary standard were diluted up to 1.0 L with dilution water to prepare the 6.3, 13, 25, and 50 mg a.i./L test concentrations. The control was dilution water only.
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia magna
- Source: ABC laboratories In-house culture
- Age at study initiation (mean and range, SD): <24 hrs
- Feeding during test: None
- Food type: In culture; P. subcapitata and wheat grass/salmon starter/yeast blend
- Frequency: In culture; Daily
ACCLIMATION
- Acclimation period: None
Study design
- Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Post exposure observation period:
- N/A
Test conditions
- Hardness:
- 142 CaCO3/L
- Test temperature:
- 19.5 to 20.2 degree C
- pH:
- 8.5 to 8.7
- Dissolved oxygen:
- 8.5 to 8.8 on fresh solutions
8.6 to 8.8 on spent solutions - Salinity:
- N/A
- Nominal and measured concentrations:
- Nominal: 0 (control), 6.3, 13, 25, 50, 100 mg a.i./L
Mean Measured: - Details on test conditions:
TEST SYSTEM
- Test vessel: 250 ml glass jars with cover
- Fill volume: 200 ml
- Aeration: None
- Renewal rate of test solution (frequency/flow rate): Daily
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Moderately hard freshwater
- Alkalinity: 154 CaCO3/L
- Conductivity: 341 uS
- Culture medium different from test medium: No
- Intervals of water quality measurement: Total hardness, total alkalinity, and conductivity measured at test initiation. Temperature, dissolved oxygen and pH were measured daily
OTHER TEST CONDITIONS
- Adjustment of pH: None
- Photoperiod: 16 hr daylight photoperiod with 30 minute dawn and dusk periods
- Light intensity: 729 lux
EFFECTS Measured: Immobility within 15 seconds after gentle agitation of test chamber or organism itself
TEST CONCENTRATIONS
- Range finding study: Yes
- Range finding concentrations: 0, 0.1, 1.0, 10 and 100 mg a.i./L
- Results used to determine the conditions for the definitive study: No mortality or immobility in any test concentration- Reference substance (positive control):
- no
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 88.4 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 88.4 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 40.7 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- At initiation, measured potassium allophonate concentrations were 95 - 107% of nominal concentrations. At 24 hrs, measured concentrations in fresh solution were 94 - 150% of the nominal concentrations. At 24 and 48hrs, measured concentrations in spent solution were 50 - 95% of the nominal concentrations. The mean measured concentrations throughout the 48 hr exposure were 5.26, 13.9, 23.4, 40.7 and 88.4 mg a.i./L.
After 48 hrs, immobility was 0, 5, 5, 0, 5 and 15% in the 0, 5.26, 13.9, 23.4, 40.7 and 88.4 mg a.i./L treatments respectively. Therefore, the 24- and 48- Hr EC50 was >88.4 mg a.i./L and the NOEC was 40.7 mg a.i./L. No sublethal effects were reported and the 48- Hr concentration-response curve could not be calculated. - Results with reference substance (positive control):
- N/A
- Reported statistics and error estimates:
- EC50 estimates were calculated by probit, Trimmed Spearman-Karber, or Spearman-Karber method with SAS software (Version 9.1). NOEC was determined using a Dunnett's test. No 95% confidence limits could be deteremined.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The D. magna 24- and 48- Hr EC50 for potassium allophonate is >88.4 mg a.i./L, the highest concentration tested. Immobility occured in 15% of the organisms at a concentration of 88.4 mg a.i./L, leading to a NOEC at 40.7 mg a.i./L.
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