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EC number: - | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2019-04-10 to 2019-04-12
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Version / remarks:
- 2004
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Remarks:
- HPLC-DAD method
- Details on sampling:
- The concentrations of the test item were analytically verified via HPLC-DAD in fresh media at the start of the exposure and at renewal (0 and 24 h) and in old media (24 and 48 h).
- Vehicle:
- no
- Details on test solutions:
- A stock solution (100 mg/L of the test item were weighed out) was freshly prepared with dilution water prior to the start of the exposure (at 0 hours) and prior to the renewal of the test solutions (at 24 hours). The stock solution was mixed thoroughly by manual agitation until the test item was completely dissolved. 5 test item concentrations in a geometric series with a separation factor of 1.78, prepared by dilution of the stock solution 100 mg/L with dilution water, were tested as follows: 9.96 - 17.7 - 31.6 - 56.2 - 100 mg/L
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- Origin: Institut für Wasser-, Boden- und Lufthygiene (WaBoLu), 14195 Berlin, Germany
Breeder: Noack Laboratorien GmbH, Käthe-Paulus-Str. 1, 31157 Sarstedt, Germany
Culture medium: Elendt M4, according to OECD 202, Annex 3 (2004), is used. - Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Hardness:
- 285-298 mg CaCO3/L
- Test temperature:
- 20.2-20.7°C
- pH:
- 24h: 7.66-7.76; 48h: 7.40-7.80
- Dissolved oxygen:
- 24h: 8.12-8.66; 48h: 6.89-7.69
- Conductivity:
- 639-681 µS/cm
- Nominal and measured concentrations:
- Nominal concentrations: 9.96 - 17.7 - 31.6 - 56.2 - 100 mg/L;
Measured concentrations (fresh media ): 10.5 -18.3 - 33.8 - 58.8 -106 mg/L;
Measured concentrations (old media ): 9.62 - 16.75 - 30.65 - 54.9 - 98.15 mg/L. - Reference substance (positive control):
- yes
- Remarks:
- The reference substance Potassium dichromate was determined after 24 hours from 2019-03-06 to 2019-03-07.
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 25.2 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- 59.4 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- See Table 1
- Results with reference substance (positive control):
- The 24h-EC50 value was 2.17 mg/L (valid range: 0.6-2.4 mg/L, acc. to AQS P 9/2 (02/2000).
- Validity criteria fulfilled:
- yes
- Conclusions:
- The short term toxicity to Daphnia magna was determined and the 48h-EC50 value was 25.2 mg/L.
- Executive summary:
The acute immobilization effect of the test item to Daphnia magna (STRAUS) was performed according to OECD guideline 202 (2004) and GLP. The study was conducted in a closed system without headspace under semi-static conditions over a period of 48 hours. Based on the results of a preliminary study, five concentrations within a range of 9.96-100 mg/L ina gerometric series with a separation factor of 1.78 were tested. Twenty daphnids (divided into 4 replicates with 5 daphnids each) were exposed to each concentration level and the control (test medium without test item). The concentrations of the test item were analytically verified via HPLC-DAD in fresh media at the start of the exposure and at renewal (0 and 24 h) and in old media (24 and 48 h). The measured concentrations were within the range of ±20% of the nominal values, the effect concentrations were given on the basis of the nominal concentrations. The 48h-EC50 value was determined to be 25.2 mg/L.
Reference
Table 1: Immobilization rates after 24 and 48 hours of Exposure in the definitive test.
Nominal concentration [mg/L] | Immobilization [%] | |||||||||
24 hours | 48 hours | |||||||||
Replicates | Replicates | |||||||||
1 | 2 | 3 | 4 | Mean | 1 | 2 | 3 | 4 | mean | |
100 | 80 | 80 | 100 | 80 | 85 | 100 | 100 | 100 | 100 | 100 |
56.2 | 40 | 0 | 0 | 80 | 30 | 40 | 80 | 80 | 100 | 75 |
31.6 | 0 | 0 | 0 | 0 | 0 | 40 | 80 | 60 | 60 | 60 |
17.7 | 21 | 0 | 0 | 0 | 5 | 20 | 20 | 80 | 60 | 45 |
9.96 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Control | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Description of key information
An acute immobilization effect of the test item to Daphnia magna (STRAUS) was performed according to OECD guideline 202 (2004) and GLP. The study was conducted in a closed system without headspace under semi-static conditions over a period of 48 hours. Based on the results of a preliminary study, five concentrations within a range of 9.96-100 mg/L ina gerometric series with a separation factor of 1.78 were tested. Twenty daphnids (divided into 4 replicates with 5 daphnids each) were exposed to each concentration level and the control (test medium without test item). The concentrations of the test item were analytically verified via HPLC-DAD in fresh media at the start of the exposure and at renewal (0 and 24 h) and in old media (24 and 48 h). The measured concentrations were within the range of ±20% of the nominal values, the effect concentrations were given on the basis of the nominal concentrations. The 48h-EC50 value was determined to be 25.2 mg/L.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Dose descriptor:
- EC50
- Effect concentration:
- 25.2 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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