Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 413-750-2 | CAS number: 171090-93-0 ANOX 1315; ANOX BF; DURAD AX 38
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Long-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- long-term toxicity to fish, other
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 4 June 1997 - 25 June 1997
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 204 (Fish, Prolonged Toxicity Test: 14-day Study)
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- no
- Vehicle:
- not specified
- Details on test solutions:
- Identity and concentration of auxiliary solvent for dispersal: Acetone: 33 µl/l
- Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- Species: Brachydanio rerio.
Justification for the selection of the test system: the Brachydanio rerio was chosen as a Cyprinidae species, since it is widely accepted by Health Authorities as an experimental model with documented susceptibility to a wide range of toxic substances.
Number: 10 animals/concentration
Body weight and length (at the beginning of the study): 0. 330 g and about 2.7 cm (average values).
Supplier: F.lli Richiardi, Gaglianico (BI) - Shipping slip no. 04/A dated March 27, 1997
Water: reconstituted water with a hardness of about 236 mg/l of CaCO3, a Ca/Mg rate of 4:1 and a Na/K of 10:1.
ANIMAL HUSBANDRY
Keeping of fish
All fish are kept (in a glass aquarium) in water having the following characteristics:
- Temperature: 23˚C
- pH: about 7.8
- Hardness: about 236 mg/l of CaCO3
- Dissolved O2 concentration: 8.1 mg/l
The fish are exposed to artificial lighting with a light period of 16 hours daily and are fed once a day with Mangime SERA (Aquaristik GmbH).
Supply and acceptance of fish
The fish were purchased in excess of the number foreseen for the study. On arrival all fish were kept in a glass aquarium and following a 48-hour setting in period, no mortality was recorded after 7 days of acclimation.
Study acclimation
After acceptance selected fish are kept for a further period of 5-7 days before use, to complete the acclimation.
Identification of the experimental groups
During the experiment the fish are kept in silylated glass beakers containing 5 liters of test article solution or control. Beaker labels gave experiment number and concentration group and color tag. Each beaker was replaced by a new one each time the test article solution and control were changed. - Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 21 d
- Hardness:
- 236 mg CaCO3/L
- Test temperature:
- 22˚C
- pH:
- 7 - 8.3
- Dissolved oxygen:
- 8.1 mg/L
- Salinity:
- Not specified
- Conductivity:
- Not specified
- Nominal and measured concentrations:
- Nominal concentrations of 0.033, 0.104, 0.33 mg/l/day
- Key result
- Duration:
- 21 d
- Dose descriptor:
- LC50
- Effect conc.:
- > 0.33 mg/L
- Nominal / measured:
- nominal
- Basis for effect:
- mortality
- Key result
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.33 mg/L
- Nominal / measured:
- nominal
- Basis for effect:
- mortality
- Details on results:
- OBSERVATION OF VISIBLE ABNORMALITIES
No animals of the control and the treated group showed visible signs of abnormalities.
MORTALITY
No animals of the controls and the treated groups died.
BODY WEIGHT
No effects
PARAMETER CHECKING
The values of dissolved oxygen, pH and temperature always remained within the acceptable limits.
LC50 AND ITS STATISTICAL LIMITS, LOEC AND NOEC
LC50 was not calculable at any time.
LOEC: was not calculable
NOEC: for lethal and other statistically significant effects was 0. 33 mg/l. - Validity criteria fulfilled:
- yes
- Conclusions:
- Experimental data from a prolonged toxicity study in which fish were treated with the test article ANOX BF at the nominal concentrations of 0, 0.033, 0.104 and 0.33 mg/l/day.
The animals were treated and observed for 21 (1 4+7) days.
No animals in the control and in the treated groups died or showed signs of visible abnormalities.
The LC50 value was not calculable at any time.
LOEC was not calculable at any time.
NOEC for lethal and other than lethal effects was always 0.33 mg/l (nominal concentration). - Executive summary:
The purpose of this study was to determine at selected intervals the threshold levels of lethal and other observable effects and the NOEC in fish after prolonged exposure to the test article ANOX BF.
The test method is in accordance with Organisation for Economic Cooperation and Development Guidelines (section 2, subpart 204, Paris 1984).
Experimental data from a prolonged toxicity study in which fish were treated with the test article ANOX BF at the nominal concentrations of 0, 0.033, 0.104 and 0.33 mg/l/day.
The animals were treated and observed for 21 (1 4+7) days.
No animals in the control and in the treated groups died or showed signs of visible abnormalities.
The LC50 value was not calculable at any time.
LOEC was not calculable at any time.
NOEC for lethal and other than lethal effects was always 0.33 mg/l (nominal concentration).
Reference
Comments: Concentration analyses of the test solutions were performed and samples withdrawn at 0 and 24 h of the first and the last day of treatment with the HPLC method (see 1.4.10). The test substance disappeared, therefore all calculations are based on nominal concentrations. A later stability test on aqueous solutions of Anox BF stated that the most reasonable explanation for the loss of substance in aqueous solution is the hydrolysis of the esteric function. This hydrolysis process would lead to the formation of a mixture of the C-14 and C-15 alcohols and 3-(3'-5' di t- butyl-4' hydroxylphenyl)propionic acid. However due to the low concentrations of these decomposition products it has not been possible to confirm their presence in solution.
Description of key information
The LC50 value and LOEC was not calculable at any time. NOEC for lethal and other than lethal effects was 0.33 mg/l (nominal concentration).
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 100 mg/L
Additional information
The purpose of this study was to determine at selected intervals the threshold levels of lethal and other observable effects and the NOEC in fish after prolonged exposure to the test article ANOX BF.
The test method is in accordance with Organisation for Economic Cooperation and Development Guidelines (section 2, subpart 204, Paris 1984).
Experimental data from a prolonged toxicity study in which fish were
treated with the test article ANOX BF at the nominal concentrations of
0, 0.033, 0.104 and 0.33 mg/l/day.
The animals were treated and observed for 21 (1 4+7) days.
No animals in the control and in the treated groups died or showed signs
of visible abnormalities.
The LC50 value was not calculable at any time.
LOEC was not calculable at any time.
NOEC for lethal and other than lethal effects was always 0.33 mg/l
(nominal concentration).
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.