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Diss Factsheets
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EC number: - | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- effects on growth of green algae
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- From February 20 to 26, 2021
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Freshwater Alga and Cyanobacteria, Growth Inhibition Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- Batch number: CN0191205
Purity: 98.3% - Analytical monitoring:
- yes
- Details on sampling:
- Samples were taken for analysis from the freshly prepared solutions at 0 hour and the old solutions at 72 hours.
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The test solutions were prepared by dilution of a stock solution. A stock solution of test item at a nominal concentration of 100 mg/L was prepared with OECD alga growth medium. 0.1003 g of test item was weighed and dissolved in 1000 mL of growth medium by magnetic stirring for about 24 hours. After magnetic stirring, undissolved test item was observed in the solution, the solution was filtered through a 0.45 μm filter membrane and the filtrated solution was used as the highest concentration (100% of 100 mg/L). Pipette 5, 16, 50, 160 mL of the filtrated solution and make up to 500 mL with OECD alga growth medium. The solutions were mixed thoroughly, resulting in test solutions at 1%, 3.2%, 10%, 32% of the saturated solution prepared at 100 mg/L. - Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- TEST ORGANISM
- Common name: green alga
- Strain: Desmodesmus subspicatus
- Source (laboratory, culture collection): The stock alga was originally introduced from Institute of Hydrobiology, Chinese Academy of Sciences
- Age of inoculum (at test initiation): four days
- Method of cultivation:
ACCLIMATION
- Acclimation period: four days
- Culturing media and conditions (same as test or not): same
- Any deformed or abnormal cells observed: - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
- Test temperature:
- 21-24 oC
- pH:
- 7.75 - 8.97
- Nominal and measured concentrations:
- nominal concentrations: 1%, 3.2%, 10%, 32% and 100% of the saturated solution prepared at 100 mg/L; measured concentrations: 0.246, 0.800, 2.22, 7.89 and 27.5 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: flask
- Type (delete if not applicable): closed
- Material, size, headspace, fill volume: 100 mL
- Aeration:
- Type of flow-through (e.g. peristaltic or proportional diluter):
- Renewal rate of test solution (frequency/flow rate): no
- Initial cells density: 5E+03 cell/mL
- Control end cells density: 3.25E+05 cell/mL
- No. of organisms per vessel:
- No. of vessels per concentration (replicates): 3 replicates
- No. of vessels per control (replicates): 3 replicates
- No. of vessels per vehicle control (replicates):
GROWTH MEDIUM
- Standard medium used: yes
- Detailed composition if non-standard medium was used:
OTHER TEST CONDITIONS
- Sterile test conditions: yes/no
- Adjustment of pH: no
- Photoperiod:
- Light intensity and quality: 4440-8880 Lux
- Salinity (for marine algae):
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: manual cell counting with microscope
TEST CONCENTRATIONS
- Spacing factor for test concentrations:
- Justification for using less concentrations than requested by guideline:
- Range finding study
- Test concentrations: 1% and 10% of the saturated solution prepared at 100 mg/L
- Results used to determine the conditions for the definitive study:
CULTURING APPARATUS
-Details on culturing apparatus used: - Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol (3,5-DCP)
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 27.5 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 27.5 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 27.5 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- yield
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 27.5 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- yield
- Details on results:
- During the 72-hour test duration, there was no abnormal appearance of the algae observed in control replicates and replicates of each test group.
After 72 hours exposure, there was no significant inhibition of algae growth rates and yield at any concentration (inhibition < 10%). There was no significant difference in algae growth rate and growth yield between the control group and each test group (p>0.05, t-test). - Results with reference substance (positive control):
- 72 hour EyC50 of 3,5-DCP to Desmodesmus subspicatus was 2.0 mg/L with the 95% confidence limits 2.0 - 2.1 mg/L and 72 hour ErC50 was 2.6 mg/L with the 95% confidence limits 2.5 - 2.8 mg/L.
- Validity criteria fulfilled:
- yes
- Conclusions:
- The 72-hour effect concentration of average specific growth rate (ErCx) of and yield (EyCx) of the test item to Desmodesmus subspicatus were: ErC50 and EyC50 is greater than 27.5 mg/L, NOEC for growth rate and yield is 27.5 mg/L.
- Executive summary:
An alga growth inhibition study of PF-06452787 (BOC Ketone) was conducted over a 72-hour exposure period in a static system.
The 72-hour effect concentration of average specific growth rate (ErCx) of and yield (EyCx) of the test item to Desmodesmus subspicatus were: ErC50 and EyC50 is greater than 27.5 mg/L, NOEC for growth rate and yield is 27.5 mg/L.
Reference
Description of key information
An alga growth inhibition study of PF-06452787 (BOC Ketone) was conducted over a 72-hour exposure period in a static system.
The 72-hour effect concentration of average specific growth rate (ErCx) of and yield (EyCx) of the test item to Desmodesmus subspicatus were: ErC50 and EyC50 is greater than 27.5 mg/L, NOEC for growth rate and yield is 27.5 mg/L.
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 27.5 mg/L
- EC10 or NOEC for freshwater algae:
- 27.5 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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