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EC number: 831-167-5 | CAS number: 2126827-44-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 05 Mar 2019 to 04 Apr 2019
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 019
- Report date:
- 2019
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- GLP compliance:
- yes
Test material
- Reference substance name:
- 2,9,16,23-tetrakis(2,5-di-tert-butyl-4-methoxyphenoxy)phthalocyanine
- EC Number:
- 831-167-5
- Cas Number:
- 2126827-44-7
- Molecular formula:
- C92H106N8O8
- IUPAC Name:
- 2,9,16,23-tetrakis(2,5-di-tert-butyl-4-methoxyphenoxy)phthalocyanine
- Test material form:
- solid
Constituent 1
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: 2715/18/01
- Expiration date of the lot/batch: 01 July 2020
- Purity: 99%:
- CAS number: 2126827-44-7
STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: At room temperature protected from light
- Stability under test conditions: Stable
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge: Municipal sewage treatment plant: 'Waterschap Aa en Mass','s Hertogenbosch, The Netherlands, receiving predominantly domestic sewage
- Storage conditions: Continuously aerated
- Concentration of sludge: 3.4 g/L suspended solids
- Pre-treatment of slduge: sludge was coarsely sieved (1mm) - Duration of test (contact time):
- 28 d
Initial test substance concentrationopen allclose all
- Initial conc.:
- 16 mg/L
- Based on:
- test mat.
- Initial conc.:
- 12 mg/L
- Based on:
- TOC
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- CO2 evolution
- Details on study design:
- TEST CONDITIONS
- Composition of medium: Tap water putified by reverse osmosis (Milli-RO) and subsequently passed over activated carbon. Used to prepare standard mineral medium.
- Test temperature: 22.2 to 22.8°C
- pH: 7.6 to 7.7
- pH adjusted: not stated
- Suspended solids concentration: 10mg/l
- Continuous darkness: yes, in addition to brown vessels
TEST SYSTEM
- Culturing apparatus: 2 litre brown test glass bottles, test item was added via weighed amounts of small watch glasses, this was performed under low light conditions.
- Number of culture flasks/concentration: 2 test flasks with test item and inoculum
- Method used to create aerobic conditions: aeration and continual stirring
- Test performed in closed vessels due to significant volatility of test substance: not stated
- Details of trap for CO2 and volatile organics if used: CO2 was purged from synthetic air used to aerate the system using barium hydroxide before aerating. After the test vessels, three OC2 absorbers (bottles filled with 100ml 0.0125M Ba(OH)2) were connected in series to the exit air line of each test bottle.
SAMPLING
- Sampling frequency: 2, 5, 8, 12, 15, 19, 23, 28 (measured on 29). Titrations for the procedure and toxicity control were made over a period of 14 days.
- Sampling method: The amount of CO2 produced was determined by titrating remaining Ba(OH)2 with 0.05 M standardized HCl (1:20 dilution from 1 M HCl (Titrisol® ampoule), Merck, Darmstadt, Germany). Each time the CO2-absorber nearest to the test bottle was removed for titration; each of the remaining two absorbers were moved one position in the direction of the test bottle. A new CO2-absorber was placed at the far end of the series. Phenolphthalein (1% solution in ethanol, Merck) was used as pH-indicator. On the penultimate day, pH of respective test suspensions was measured and 1 mL of concentrated HCl (37%, Merck) was added to the inoculum blank and test suspension. Bottles were aerated overnight to drive off CO2 present in the test suspension. Final titration was made on day 15 (procedure and toxicity control) and on day 29 (remaining vessels).
CONTROL AND BLANK SYSTEM
- Inoculum blank: 2 bottles containing only inoculum.
- Procedure control: duration of test 14 days, last CO2 measurements day 15, 1 bottle containing test item, and inoculum.
- Toxicity control: duration of test 14 days, last CO2 measurements day 15, 1 bottle containing test item, reference item and inoculum.
Reference substance
- Reference substance:
- other: sodium acetate
- Remarks:
- CAS number 127-09-3
Results and discussion
- Preliminary study:
- Not applicable
% Degradation
- Key result
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 1
- Sampling time:
- 28 d
- Remarks on result:
- other:
- Remarks:
- individual vessel
- Details on results:
- -Toxicity control: 41% biodegradation occurred within 14 days. This indicates the test item was not inhibited by microbial activity.
BOD5 / COD results
- Results with reference substance:
- - Sodium acetate biodegraded 83% within 14 days which is within the criteria for acceptibility of the test.
Any other information on results incl. tables
CO2 production and percentage biodeegradation of the test item (Bottle A):
Day | 0.05 N HCl titrated (mL) - Blank (mean) | 0.05 N HCl titrated (mL) - Bottle A | Produced CO2 (mL HCl) | Produced CO2 (mg) | Cumulative CO2 (mg) | Biodegradation (%)b |
2 | 42.09 | 42.20 | 0.0 | 0.0 | 0.0 | 0 |
5 | 39.11 | 39.55 | 0.0 | 0.0 | 0.0 | 0 |
8 | 37.74 | 38.06 | 0.0 | 0.0 | 0.0 | 0 |
12 | 37.73 | 36.80 | 0.92 | 1.0 | 1.0 | 1 |
15 | 37.58 | 38.53 | 0.0 | 0.0 | 1.0 | 1 |
19 | 36.08 | 37.52 | 0.0 | 0.0 | 1.0 | 1 |
23 | 37.04 | 39.01 | 0.0 | 0.0 | 1.0 | 1 |
29 a | 35.71 | 35.87 | 0.0 | 0.0 | 1.0 | 1 |
29 a | 39.62 | 39.85 | 0.0 | 0.0 | 1.0 | 1 |
29 a | 41.26 | 41.47 | 0.0 | 0.0 | 1.0 | 1 |
CO2 production and percentage biodegradation of the test item (Bottle B)
Day | 0.05 N HCl titrated (mL) - Blank (mean) | 0.05 N HCl titrated (mL) - Bottle A | Produced CO2 (mL HCl) | Produced CO2 (mg) | Cumulative CO2 (mg) | Biodegradation (%)b |
2 | 42.09 | 42.65 | 0.0 | 0.0 | 0.0 | 0 |
5 | 39.11 | 39.85 | 0.0 | 0.0 | 0.0 | 0 |
8 | 37.74 | 38.53 | 0.0 | 0.0 | 0.0 | 0 |
12 | 37.73 | 37.18 | 0.54 | 0.6 | 0.6 | 1 |
15 | 37.58 | 38.85 | 0.0 | 0.0 | 0.6 | 1 |
19 | 36.08 | 37.79 | 0.0 | 0.0 | 0.6 | 1 |
23 | 37.04 | 39.12 | 0.0 | 0.0 | 0.6 | 1 |
29a | 35.71 | 37.68 | 0.0 | 0.0 | 0.6 | 1 |
29a | 39.62 | 41.27 | 0.0 | 0.0 | 0.6 | 1 |
29a | 41.26 | 41.03 | 0.23 | 0.3 | 0.9 | 1 |
a - CO2 measured on day 29 is actually part of CO2 production of day 28, since microbial activity was ended on day 28 by
addition of HCl.
b - Calculated as the ratio between CO2 produced (cumulative) and the ThCO2 of the test item: 89.3 mg CO2/2L.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- not readily biodegradable
- Conclusions:
- The test item was measured to undergo 1% biodegradation over 28 days, and is therefore considered to be not readily biodegradable under the conditions of the test. The test substance had no inhibitory effect on degradation, and the reference chemical, sodium acetate, was degraded by >40% within 14 days.
- Executive summary:
A reliable study to determine the ready biodegradability of the test item following the internationally acceptable test guideline OECD 301B was carried out according to GLP. The amount of CO2 produced by the test vessels was determined by titration of remaining Ba(OH)2 in the scrubber vessels (located after the test vessels) with 0.05 M standardized HCl. The test item was degraded by <5% (1% for both test vessels) after 28 days and was not readily biodegradable under the conditions of the test (CO2 evolution, modified Sturm test). The test validity criteria were met and there were no significant deviations noted.
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