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EC number: 211-322-8 | CAS number: 638-16-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2006-06-14 to 2006-06-16
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 007
- Report date:
- 2007
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Qualifier:
- according to guideline
- Guideline:
- other: "Method concerning tests relating to new chemical substances"
- Version / remarks:
- No.1121002 by the Pharmaceutical and Food Safety Bureau, 2003; No.2 by the Manufacturing Industries Bureau, 2003; No. 031121002 Environmental Health Department, Policy Division
- Principles of method if other than guideline:
- method similar to OECD202 performed under GLP; with analytical verification of test substance
- GLP compliance:
- yes
Test material
- Reference substance name:
- 1,3,5-triazine-2,4,6(1H,3H,5H)-trithione
- EC Number:
- 211-322-8
- EC Name:
- 1,3,5-triazine-2,4,6(1H,3H,5H)-trithione
- Cas Number:
- 638-16-4
- Molecular formula:
- C3H3N3S3
- IUPAC Name:
- 1,3,5-triazine-2,4,6(1H,3H,5H)-trithione
- Test material form:
- solid: particulate/powder
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: All concentrations were sampled at test start and test end.
- Sample storage conditions before analysis: Instant analysis
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: A stock solution of 100 mg/L was prepared and diluted with dilution water for each concentration level. For the highest test concentration, the stock solution was used without dilution.
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia magna
- Source: Ministry of the Environment, Institute for Environmental Studies, Japan; date of receipt: 04 August 1997
- Age at study initiation (mean and range, SD): < 24 h
- Method of breeding: Subculture breeding, maximum breeding period: 4 weeks
- Feeding during test: None
CULTURE
- Type and amount of food: P. subcapitata, 0.1 - 0.2 mg C per daphnia
- Feeding frequency: 3 - 5 days per week
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
Test conditions
- Hardness:
- 200-250 mg/L (as CaCO3 equivalent)
- Test temperature:
- 19.8° C - 20.4° C
- pH:
- 6.5 - 7.8
- Dissolved oxygen:
- 8.6 - 9.0 mg/L
- Nominal and measured concentrations:
- Nominal concentrations: 0 mg/L, 6.3 mg/L, 12.5 mg/L, 25.0 mg/L, 50.0 mg/L, 100.0 mg/L
Measured concentrations (geometric mean after 48h exposure): < 0.15 mg/L, 6.0 mg/L, 12.4 mg/L, 25.0 mg/L, 50.3 mg/L, 102.5 mg/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: Beakers
- Type (delete if not applicable): covered
- Material, size, fill volume: Glass, 100 mL, 80 mL
- Aeration: None, dilution water was aerated before test start (dissolved oxygen is more than 3 mg/L during exposure)
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Elendt M7 medium was used.
- Culture medium different from test medium: no
- Intervals of water quality measurement: Temperature, oxygen, pH and hardness were measured at test start and after 48 h.
OTHER TEST CONDITIONS
- Photoperiod: 16 h light/8 h dark
- Light intensity: 800 - 1200 lux
EFFECT PARAMETERS MEASURED (with observation intervals if applicable):
- Immobilisation was observed every 24 h.
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2
- Range finding study
- Test concentrations: 25, 50 and 100 mg/L (nominal), 25.6, 52.0 and 105.1 mg/L (measured)
- Results used to determine the conditions for the definitive study: EC50 (48h) 42.3 mg/L - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate
Results and discussion
Effect concentrationsopen allclose all
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 38 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: 95% CL 31.6 to 45.7 mg/L
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 52.1 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- 1,3,5-triazine-2,4,6(1H,3H,5H)-trithione, trisodium salt (TMT)
- Remarks on result:
- other: recalculated value
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- 50.1 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- - Mortality of control: No mortalities
- Immobilisation of daphnia after 48 h of exposure: 10% at 25 mg/L, 80% at 50.3 mg/L, 100% at 102.5 mg/L
- 0% immobilisation maximum concentration: 12.4 mg/L after 48 h; 100% immobilisation minimum concentration: 102.5 mg/L.
- Analytics: Measured concentrations ranged from 96.8 to 99.1% of nominal values at test start and from 95.2 to 106.1% of nominal values at test end. The concentration of the test material at the time of initial exposure was within ± 20% of the established concentration range. The test material concentration in the test liquid wa within ± 20% of the initial concentration during the exposure period (48 h). - Results with reference substance (positive control):
- - Results with reference substance valid? Yes
- EC50 (48): 0.9 mg/L (95% CL: 0.74 - 1.10 mg/L)
- Date of experiment: 12 Mar - 14 Mar 2006 - Reported statistics and error estimates:
- EC50 values were calculated using the Probit method.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The EC50 after 48 hours incubation based on mobility on Daphnia magna was 38 mg/L (95% confidence interval: 31.6 to 45.7 mg/L).
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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