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EC number: 266-235-8 | CAS number: 66204-44-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1995
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Remarks:
- Study according to guideline (OECD 201, EG C.3) in compliance with GLP. In the vessels with the highest applied test substance concentration (23.43 mg/L) the pH value deviated by more than 1 unit during the test. This deviation is considered to have no influence on the integrity of the results.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 995
- Report date:
- 1995
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Deviations:
- yes
- Remarks:
- In the vessels with the highest applied test substance concentration (23.43 mg/L) the pH value deviated by more than 1 unit during the test.
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.3 (Algal Inhibition test)
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- Arbeitsgemeinschaft GAB/IFU GmbH
Test material
- Reference substance name:
- 3,3'-methylenebis[5-methyloxazolidine]
- EC Number:
- 266-235-8
- EC Name:
- 3,3'-methylenebis[5-methyloxazolidine]
- Cas Number:
- 66204-44-2
- Molecular formula:
- C9H18N2O2
- IUPAC Name:
- 3,3'-methylenebis[5-methyloxazolidine]
- Details on test material:
- - Test material: Reaction products of paraformaldehyde and 2-hydroxypropylamine (ratio of 3:2)
- Lot/Batch number: Batch number: NA 2571
- Specification: MAR 71
- Clear liquid; infinitely soluble in water; density ca. 1.06 kg/L
- Purity: 95% (w/w)
- Further relevant properties: vapour pressure < 1 hPa
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- Nominal concentrations 4.8 and 23.4 mg/l were measured as fresh solution and after 24, 48, and 72 h.
Test organisms
- Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- - Species: Scenedesmus subspicatus CHODAT (current name Desmodesmus subspicatus)
- Strain: No data
- Source: Origin: Pflanzenphysiologisches Institut der Universität Göttingen (Germany)
- Laboratory culture: Yes
- Method of cultivation: Stock cultures were continuously grown in aerated semi-static liquid cultures. Stock cultures also kept on slant agar tubes and freshly passaged about once a month.
- Pretreatment: No data
- Initial cell concentration: 104 cells/mL
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
Test conditions
- Test temperature:
- 21.0 – 25.0 °C
- pH:
- 7.96 – 9.22
- Nominal and measured concentrations:
- Nominal concentrations:
- Range-finding test: 0.01, 0.1, 1, 10, 100 mg/L + control
- Main test: 1.0, 2.2, 4.84, 10.65, 23.43 mg/L + control (nominal concentrations)
Measured concentrations:
- Test substance measured in assays with 0, 4.84 and 23.43 mg/L at different timepoints without inoculation (For values see table below) - Details on test conditions:
- GROWTH MEDIUM
- Standard medium used: yes (OECD TG 201 medium)
TEST SYSTEM
- Volume of culture flasks: 1000 mL Duran bottles, 500 mL test volume
- Light quality: 2 light tubes (OSRAM L 36W/25, DIN IEC 81, light colour 25, universal white)
- Number of vessels/ concentration: 3 (test substance); 6 (control)
OTHER TEST CONDITIONS
- Aeration of dilution water: The algae were supplied with CO2 by aeration. Air was filtered through activated carbon and sterile membrane filters.
SAMPLING
- Cells counted after 24, 48 and 72 h
- pH measured at beginning and end of test. Temperature measured after 0, 24, 48 and 72 h.
TEST PARAMETER
- Growth rate, biomass
STATISTICS
- Mean value for each MAR 71 concentration and control plotted versus time. EbC, ErC and NOEC values calculated by log-linear regression analysis. - Reference substance (positive control):
- no
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 2.2 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 2.1 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 5.7 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 1 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 1.3 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 2.6 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Details on results:
- - No other effects obseved
Any other information on results incl. tables
Results of main test:
Test substance [mg/l] |
Cell concentrations (mean values) |
|||||
measured |
Inhibition Percent |
|||||
0 h |
24 h |
48 h |
72 h |
biomass |
growth |
|
control |
1.0 |
5.73 |
22.92 |
121.6 |
|
|
1.0 |
1.0 |
3.13 |
25.52 |
144.3 |
-13.0 |
-3.8 |
2.2 |
1.0 |
2.61 |
15.10 |
81.25 |
35.8 |
9.0 |
4.84 |
1.0 |
2.60 |
2.61 |
12.50 |
89.7 |
48.3 |
10.65 |
1.0 |
0.00 |
1.56 |
3.65 |
99.0 |
73.3 |
23.43 |
1.0 |
0.00 |
1.56 |
0.52 |
100.8 |
96.9 |
Temp.[°C] |
21.0-25.0 |
24.0-25.0 |
23.0-25.0 |
23.0-25.0 |
||
pH |
7.96-9.22 |
|
|
7.96-9.15 |
Cell concentrations are mean of 3 parallels.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The substance was found to be harmful towards algae at relatively low concentrations.
- Executive summary:
The substancewas found to be acutely toxic towards algae at relatively low concentrations.The numerical results are given below; all results refer to the nominal concentrations.All validity criteria according the test Guideline are met within the study.
A clear concentration-response relationship can be established from the study results.
The constituents of the reaction product (substance) are not volatile from aqueous solutions, therefore this removal mechanism could not influence the test results. It is not expected that the the low adsorptivity of the test substance influenced test substance concentrations.
The constituents of the reaction product (substance) hydrolyse completely in concentrations which are expected to occur in waste waters and surface waters. Also in the media of toxicity tests, complete hydrolysis is expected, therefore the observed effects are assumed to be caused by a mixture of hydrolysis products.
Analytical measurements of the formaldehyde content in the test substance was not successful. During the test period an increase of aldehyde groups was observed which was probably caused by injection of aldehydes via the aeration system. Measurements in test solutions of the tests to daphnia and fish revealed that the test substance was stable during the test period. Therefore, in the present test the effective concentrations can be considered to be equal to the nominal concentrations.
In the vessels with the highest applied test substance concentration (23.43 mg/L) the pH value deviated by more than 1 unit during the test, but not in the vessels with lower concentrations. Therefore, this deviation should not influence the calculated effect values.
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