Schiff bases, C11-14-tert-alkyl methylene

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

48 hours

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Justification for type of information:

Guideline study, 2018

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

yes (incl. QA statement)

Specific details on test material used for the study:

Test Substance: Reaction products of C11-14 tert-alkylamine and formaldehyde
Activity: >98% active
Appearance: Straw/yellow, clear bright liquid

Analytical monitoring:

yes

Details on sampling:

Analysis of the test preparations at 0 and 48 hours

Vehicle:

no

Test organisms (species):

Daphnia magna

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Hardness:

156 as mg CaCO3

Test temperature:

20 C

pH:

8.2 - 8.4

Nominal and measured concentrations:

nominal concentrations, 0.1, 0.2, 0.4, 0.8, 1.6 and 3.2 mg/L
measured concentrations 0.11, 0.24, 0.48, 0.87, 1.6 and 2.9 mg/L

Details on test conditions:

At study initiation 5, less than 24 hours old, daphnids were randomly added to each replicate test and control vessel. Four replicate test and control vessels were prepared.
Photoperiod of 16 hours light and 8 hours darkness

Reference substance (positive control):

yes

Remarks:

potassium dichromate

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

0.32 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

< 0.11 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

1.6 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

Immobilisation of 20% was observed in the lowest tested concentration of 0.11 mg/l
The highest tesed concentrations led to 100% immobilisation

Validity criteria fulfilled:

yes

Conclusions:

The EC50 was determined to be 0.32 mg/l
Immobilisation of 20% was observed in the lowest tested concentration of 0.11 mg/l
The highest tested concentrations led to 100% immobilisation

Description of key information

The acute toxicity to Daphnia for the registered substance was 0.32 mg/l

To cover for vertebrate teting (fish) and prolonged toxicity to Daphnia similar substances have been assessed and to help confirm similiarities in properties, the acute Daphnia for these are also described;

C12-Dimethyldodecylamine = EC50 0.083

Formaldehyde, reaction products with ethylenediamine = EC50 40 mg/l

Formaldehyde, reaction products with ethylenediamine is considered to be sufficiently similar to help in the weight of evidence approach to environmental fate and effects to cover certain enpoints.  However, it is noted that this si significantly less hazardous than the substance to be registered and is not a suitable surrogate for the Daphnia reproduction assay.

This endpoint is included for information purposes only; C12-Dimethyldodecylamine is considered to be more reliable for assessment of prolonged effects on Daphnia.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

0.32 mg/L