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EC number: 236-770-1 | CAS number: 13477-62-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP study according to OECD guideline
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 014
- Report date:
- 2014
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 310 (Ready Biodegradability - CO2 in Sealed Vessels (Headspace Test)
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Tetrahydro-2-isobutyl-4-methyl-2H-pyran
- EC Number:
- 236-770-1
- EC Name:
- Tetrahydro-2-isobutyl-4-methyl-2H-pyran
- Cas Number:
- 13477-62-8
- Molecular formula:
- C10H20O
- IUPAC Name:
- 4-methyl-2-(2-methylpropyl)oxane
- Details on test material:
- - Name of test material (as cited in study report): Dihydrorosenoxid
- Physical state: liquid / colorless, clear
- Analytical purity: 99.5 corr. area% as a sum of two isomers
- Lot/batch No.: 11260/14 Versuch 19 Fraktion 6-7,9-13,14
- Expiration date of the lot/batch: 03 April 2016
- Stability under test conditions: stable
- Storage condition of test material: at room temperature
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure):
Municipal activated sludge from the wastewater treatment plant of Mannheim, Germany
was collected on 19 May 2014 from the aeration tank of the plant. A suitable aliquot of
the suspension was sieved by a finely woven mesh with a mesh size of about 1 mm. To
reduce the content of inorganic carbon in the blank control the activated sludge
suspension was aerated with carbon dioxide free air for about 48 hours at 22 ± 2° C.
- Preparation of inoculum for exposure: At the day of exposure the aeration was stopped and the sludge was allowed to settle.
The supernatant was discarded and the residue of the sludge suspension was filled up
with drinking water. The sludge of the suspension was adjusted to a concentration 800
mg/L of dry weight. Aliquots of 0.5 mL were added to the test vessels to obtain a sludge
concentration of 4 mg/L dry substance in the test assays. - Duration of test (contact time):
- 28 d
Initial test substance concentrationopen allclose all
- Initial conc.:
- 20 mg/L
- Based on:
- other: TOC
- Initial conc.:
- 26 mg/L
- Based on:
- test mat.
- Details on study design:
- TEST CONDITIONS
- Composition of medium: complies with test guideline OECD 310
- Test temperature: 22 ± 2 °C.
- pH:A pH- shift in the test vessels, induced by addition of the test substance to the test
medium was checked in a preliminary test. No shift of the pH-value was detected.
The pH-value from the mineral test medium was therefore assumed for the test
assays at start of exposure.
This pH-value of the mineral medium after correction was 7.4.
TEST SYSTEM
- Culturing apparatus: 160 mL incubation bottles filled up to a
volume of 100 mL
- Number of culture flasks blank control assays (BC): 29
- Number of culture flasks test substance assays (TS): 31
- Number of culture flasks inhibition control test assays (IH): 29
- Number of culture flasks reference substance assays (RS): 17
- Number of culture flasks assays for abiotic control (PC): 5
- Measuring equipment:
- Test performed in closed vessels due to significant volatility of test substance: The bottles were closed with
butyl rubber septa gas tight.
- Test performed in open system: no
- Details of trap for CO2 and volatile organics if used: At the sampling days three bottles of each test assay were sacrificed.
1 mL 7 molar sodium hydroxide solution was injected with a syringe trough the septum in
each test bottle. The bottles were shaken for about one hour. The carbon dioxide in the
headspace, evolved by biological processes, is solved in the alkaline solution. For the
measurement at the end of exposure five test substance assays were prepared. The
single values of these test assays were used for mean value calculation of the
biodegradation degree with a limit of confidence limit of 95 %. The inorganic carbon
content of the sodium hydroxide solution used for the alkalization of the test assays was
checked and considered at the evaluation. The bottles were opened and the amount of
Total Inorganic Carbon (TIC) was determined with a carbon analyzer. The measured TICvalues
were compared with the added amount of TOC and expressed as biodegradation
degree and plotted by a biodegradation curve.
SAMPLING
- Sampling frequency: every 3-4 days
- Sample storage before analysis: The samples were analyzed on the day of sampling
without further treatment.
CONTROL AND BLANK SYSTEM
- Inoculum blank: yes
- Abiotic sterile control: yes
Reference substance
- Reference substance:
- aniline
Results and discussion
% Degradation
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 18
- St. dev.:
- 5
- Sampling time:
- 28 d
- Remarks on result:
- other: CO2 evolution measured as TIC/TOC
- Details on results:
- Dihydrorosenoxid was not readily biodegradable in this test
BOD5 / COD results
- Results with reference substance:
- Degree of biodegradation of the reference substance after 14
days, mean value: 72 % TIC/TOC
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
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